Synthesis of triacylglycerol containing conjugated linoleic acid by esterification using two blended lipases

Abstract

We have developed an efficient esterification for the synthesis of triacylglycerol (TAG) containing conjugated linoleic acids (CLA) using a blend of two powdered lipases. Two pairs of blended lipases promoted the esterification. Rhizomucor miehei lipase plus Alcaligenes sp. lipase and Penicillium camembertii MAG and DAG lipase plus Alcaligenes sp. lipase were used. At the optimal ratio of two lipases, the content of TAG containing CLA (TAG-CLA) in all glycerols reached 82-83% after 47 h using 1 wt% of lipases. With R. miehei lipase plus Alcaligenes sp. lipase, the reaction time to obtain ca. 60% of TAG-CLA was one-third of that needed with R. miehei lipase alone. The optimal ratio of two lipases differed between these two pairs. The optimal ratio was 70-80 wt% of R. miehei lipase in the last stage of the reaction, whereas it was over a wide range of 10-90 wt% for P. camembertii lipase. In the blend of R. miehei lipase plus Alcaligenes sp. lipase, activity remained very high after 10 cycles of esterification (every 47 h) and could be used in the industrial production of TAG-CLA.

Properties and biotechnological methods to produce lipids containing conjugated linoleic acid

“…An interesting idea for improving the content of CLA in structured triacylglycerols to about 83% has been presented by Hirose et al [129], in which two lipases, one with a high degree of sn-1,3 selectivity (i.e. Rhizomucor miehei or Penicillium camembertii lipase) and a second lipase with a lower selectivity towards the sn-1,3-position (lipase from Alcaligenes sp.…”

Optimisation of flavour ester biosynthesis in an aqueous system of coconut cream and fusel oil catalysed by lipase

“…This could be because higher temperatures caused adverse effects on enzyme activity through changing enzyme conformation. One study applied 40°C as the catalysis temperature for Palatase in a solvent-free system under high operation pressure (Hirose, Yamauchi-Sato, Arai, & Negishi, 2006). The different observations in that study may be due to these different reaction systems and conditions.…”

Lipase-catalyzed harvesting and/or enrichment of industrially and nutritionally important fatty acids

“…This is followed by two references for the preparation of STAG containing the esters of CLA (Entries 28 and 29; Arcos and Hill, 2000;Hirose et al, 2006). The various isomers of CLA have been reported to have numerous beneficial effects on human health and thus it is likely that research into CLA-containing lipids will continue.…”