Synthesis of Tritium-labelled Oxytocin and Lysine-vasopressin.

Carlsson, Lars; Sjöholm, Ingvar; Painter, Tammie; Marøy, Kjartan; Brunvoll, J.; Bunnenberg, E.; Djerassi, Carl; Records, Ruth

doi:10.3891/acta.chem.scand.20-0259

Cited by 12 publications

(5 citation statements)

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“…Synthetic [3H-tyrosine]oxytocin (A. B. Ferring, Malmö), prepared as described by Carlsson & Sjoholm (1966) was employed in some antibody-binding studies. A diluent buffer, 0*05 M-tris-HCl, pH 8*0, was used except where otherwise stated.…”

Section: Methodsmentioning

confidence: 99%

The Development of a Radioimmunoassay for Oxytocin: Radio-Iodination, Antibody Production and Separation Techniques

Chard¹,

Kitau²,

Landon³

1970

Journal of Endocrinology

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A simple and rapid method is described for labelling oxytocin with 131I at a high specific activity. This method is compared with those of previous workers. A satisfactory antiserum has been raised by direct intra-lymph node injection of oxytocin adsorbed to carbon microparticles. A number of methods for separating antibody-bound from free oxytocin are described, and reasons given for preferring a procedure using ammonium sulphate precipitation. These data form the basis for developing a radioimmunoassay intended for the determination of oxytocin in human plasma.

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Section: Methodsmentioning

confidence: 99%

The Development of a Radioimmunoassay for Oxytocin: Radio-Iodination, Antibody Production and Separation Techniques

Chard¹,

Kitau²,

Landon³

1970

Journal of Endocrinology

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“…Synthetic tritiated lysine vasopressin ([2-tyrosine[3-3H],8-lysine]-vasopressin) (Carlsson & Sjöholm, 1966), was a gift from Dr V. Pliska and was originally prepared by Ferring A. B., Sweden.…”

Section: Methodsmentioning

confidence: 99%

The Uptake of Tritiated Lysine Vasopressin by Rat and Pig Pituitary Neural Lobes in Vitro

Edwards¹

1971

Journal of Endocrinology

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Uptake of tritiated lysine vasopressin ([3H]LVP) was studied in halved neural lobes of rats (which had been given either tap water (control group) or 2 % (w/v) NaCl solution as drinking water for 4 days) as well as in slices of pig neural lobe. Uptake of radioactivity into the neural lobes was shown but analysis of the extracts of incubated lobes of both species by ion exchange chromatography showed that very little of it remained in the tissue as hormone. In addition, some radioactivity was associated with trichloroacetic acid-insoluble proteins.After 90 min of incubation, and after correction for the breakdown, the uptake of unchanged [3H]LVP, expressed as a tissue : medium ratio, was 0\m=.\14 \m=+-\ 0\ m=. \ 04 and 0\m=.\09 \ m=+-\ 0\m=.\03 (mean \ m=+-\ S.E.M.) for the saline-treated and control rats respectively, while the tissue: medium ratios for the breakdown product(s) were 6\m=.\47 \ m=+-\ 0\m=.\45 and 5\m=.\50 \m=+-\0\m=.\36. The results suggest uptake of [3H]LVP into the cell with almost complete intracellular breakdown of the hormone.

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“…V laboratořích firmy Ferring syntetizovali krátce předtím Carlsson a Sjöholm h oxytocin a 8-lysin--vasopressin specificky tritiovaný na tyrosinu v poloze 2 (cit. 28,29 ). Oproti nespecificky tritiovaným produktům se předpokládala podstatně omezená izotopová výměna s okolními molekulami vody; to se ostatně potvrdilo v dlouhodobé studii stability tritiovaného vasopressinu 30 .…”

Section: Oxytocin Vasopressin a Jejich Analoga: Výzkum Na úOchbunclassified

Neurohypophyseal Peptides from Prague and Swedish Laboratories Used as Medications: Desmopressin, Terlipressin, Carbetocin. Part 1: History of Research and Beginnings of Industrial Production

Pliška

Pařı́zek²,

Flegel

2022

Chem. Listy

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From the fifties to the seventies of the last century, the neurohypophyseal peptides oxytocin and vasopressin constituted one of the main research areas at the Institute of Organic Chemistry and Biochemistry in Prague (IOCB). A significant contribution to this area is associated with the names of František Šorm, director of the said institute, and Josef Rudinger, head of the institute's peptide laboratory. At that time, newly developed research tools enabled to synthesize structural analogues of these hormones in numerous laboratories worldwide and hence to investigate the structure-activity relationships within this peptide group. Contributions of single peptide-chain positions to the respective biological activities were identified which opened a possibility to rationalize a design of peptides with a combination of changes in several positions. Several clinically interesting peptides were synthesized in the late 1960s at the IOCB and employed as therapeutics: [(Gly)3-Cys1,Lys8]-vasopressin (Glypressin Ferring®, Terlipressin INN), 1-deamino-8-ᴅ-arginine vasopressin (Desmopressin INN, dDAVP), and later the uterotonics carbetocin (INN), widely used in obstetrics to prevent postpartum haemorrhage. Since the industrial production of peptide therapeutics was scarcely possible under the conditions of socialist economy in Czechoslovakia as well as in other countries under the Soviet influence, F. Šorm agreed to use the already established scientific contacts of IOCB with the Swedish pharmaceutical company Ferring AB and to transfer the production licences to Sweden. The license agreements were signed in 1969 and led to a quick spread of dDAVP in the substitution therapy of the central form of diabetes insipidus and, moreover, contributed to a fast upsurge of the Ferring company. Somewhat later, Glypressin was produced as a therapeutic with a prolonged action in cases of cardiovascular collapse. Contacts between Prague peptide chemists and the Ferring company lasted on a rather informal base until the end of the 1980s. After the fall of the totalitarian regime in Czechoslovakia in 1990, Ferring started a joint-venture collaboration with the newly organized Czech company Léčiva st.p. Praha in a newly established group Prague Polypeptide Institute spol. s. r.o. (later Ferring-Léčiva A.S.). A substantial part of the peptide-production capacities was then transferred to new buildings in Prague.

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Synthesis of Tritium-labelled Oxytocin and Lysine-vasopressin.

Cited by 12 publications

References 0 publications

The Development of a Radioimmunoassay for Oxytocin: Radio-Iodination, Antibody Production and Separation Techniques

The Development of a Radioimmunoassay for Oxytocin: Radio-Iodination, Antibody Production and Separation Techniques

The Uptake of Tritiated Lysine Vasopressin by Rat and Pig Pituitary Neural Lobes in Vitro

Neurohypophyseal Peptides from Prague and Swedish Laboratories Used as Medications: Desmopressin, Terlipressin, Carbetocin. Part 1: History of Research and Beginnings of Industrial Production

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