Synthesis of two thermo-sensitive copolymers forming aqueous two-phase systems

Hou, Daisong; Li, Yanjie; Cao, Xuejun

doi:10.1016/j.seppur.2013.10.044

Cited by 10 publications

(2 citation statements)

References 20 publications

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“…In most work, conventional polymer-based LBS has been replaced by using thermo-separating polymers as the phase-forming component to overwhelm the limitation of polymer-based LBS such as high viscosity and difficulties in recycling process [27,28]. Thermo-separating polymers are random, di-block, and tri-block co-polymers of ethylene oxide (EO) and propylene oxide (PO) [29].…”

Section: Polymer-based Lbsmentioning

confidence: 99%

Liquid Biphasic System: A Recent Bioseparation Technology

et al. 2020

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A well-known bioseparation technique namely liquid biphasic system (LBS) has attracted many researchers’ interest for being an alternative bioseparation technology for various kinds of biomolecules. The present review begins with an in-depth discussion on the fundamental principle of LBS and this is followed by the discussion on further development of various phase-forming components in LBS. Additionally, the implementation of various advance technologies to the LBS that is beneficial towards the efficiency of LBS for the extraction, separation, and purification of biomolecules was discussed. The key parameters affecting the LBS were presented and evaluated. Moreover, future prospect and challenges were highlighted to be a useful guide for future development of LBS. The efforts presented in this review will provide an insight for future researches in liquid-liquid separation techniques.

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Section: Polymer-based Lbsmentioning

confidence: 99%

Liquid Biphasic System: A Recent Bioseparation Technology

et al. 2020

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“…A somewhat similar, and perhaps even more elegant approach relates to carrying out two partitioning operations using an ATPS where PEG is replaced by a thermoresponsive polymer such as poly(ethylene oxide)‐poly(propylene oxide) so called “EOPO” random or co‐block polymers. These include polymers such as Tergitol®, Breox®, UCON®, Pluronic®, and novel synthetic variants [28]. Such EOPO polymers are often used in bioprocessing either upstream as antifoaming agents, or downstream as formulation agents.…”

Section: Strengths – Advantages Of Atpss For Downstream Processingmentioning

confidence: 99%

Partitioning in aqueous two‐phase systems: Analysis of strengths, weaknesses, opportunities and threats

Soares

Azevedo

Alstine

et al. 2015

Biotechnology Journal

131

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For half a century aqueous two-phase systems (ATPSs) have been applied for the extraction and purification of biomolecules. In spite of their simplicity, selectivity, and relatively low cost they have not been significantly employed for industrial scale bioprocessing. Recently their ability to be readily scaled and interface easily in single-use, flexible biomanufacturing has led to industrial re-evaluation of ATPSs. The purpose of this review is to perform a SWOT analysis that includes a discussion of: (i) strengths of ATPS partitioning as an effective and simple platform for biomolecule purification; (ii) weaknesses of ATPS partitioning in regard to intrinsic problems and possible solutions; (iii) opportunities related to biotechnological challenges that ATPS partitioning may solve; and (iv) threats related to alternative techniques that may compete with ATPS in performance, economic benefits, scale up and reliability. This approach provides insight into the current status of ATPS as a bioprocessing technique and it can be concluded that most of the perceived weakness towards industrial implementation have now been largely overcome, thus paving the way for opportunities in fermentation feed clarification, integration in multi-stage operations and in single-step purification processes.

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