Synthesis of ultraluminescent gold core–shell nanoparticles as nanoimaging platforms for biosensing applications based on metal-enhanced fluorescence

Gontero, Daniela; Veglia, Alicia V.; Bracamonte, A. Guillermo; Boudreau, Denis

doi:10.1039/c6ra27649k

Cited by 43 publications

(22 citation statements)

References 26 publications

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“…It is expected that an increased electric field effect is proportional to surface plasmons' augmentation up to an optimal nanomaterial concentration for sufficient FL signal enhancement, above which the quenching effect is favored (Figure 2a) [15,19]. The latter is ascribed to intrinsic short-range molecular interactions between the produced fluorophore and the ZnO nanostructures [33,34]. Figure 2c depicts NAGase-specific catalytic reaction output within mastitic milk samples with respect to catalyst content augmentation for ZnO-QDs-SiO 2 and ZnO-NPs-SiO 2 .…”

Section: Fl Amplification Characterizationmentioning

confidence: 99%

Amplified Fluorescence by ZnO Nanoparticles vs. Quantum Dots for Bovine Mastitis Acute Phase Response Evaluation in Milk

Nirala

Shtenberg

2020

Nanomaterials

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Bovine mastitis (BM) is a prominent inflammatory disease affecting the dairy industry worldwide, originated by pathogenic agent invasion onto the mammary gland. The early detection of new BM cases is of high importance for infection control within the herd. During inflammation, various biomarkers are released into the blood circulation, which are consequently found in milk. Herein, the lysosomal activity of N-acetyl-β-d-glucosaminidase (NAGase), a predominant BM indicator, was utilized for highly sensitive clinical state differentiation. The latter is achieved by the precise addition of tetraethyl orthosilicate-coated zinc oxide nanostructures (quantum dots or nanoparticles, individually) onto a conventional assay. Enhanced fluorescence due to the nanomaterial accumulative near-field effect is achieved within real milk samples, contaminated with Streptococcus dysgalactiae, favoring quantum dots over nanoparticles (>7-fold and 3-fold, respectively), thus revealing significant differentiation between various somatic cell counts. The main advantage of the presented sensing concept, besides its clinically relevant concentrations, is the early bio-diagnostic detection of mastitis (subclinical BM) by using a simple and cost-effective experimental setup. Moreover, the assay can be adapted for BM recovery prognosis evaluation, and thus impact on udder health status, producing an alternative means for conventional diagnosis practices.

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Section: Fl Amplification Characterizationmentioning

confidence: 99%

Amplified Fluorescence by ZnO Nanoparticles vs. Quantum Dots for Bovine Mastitis Acute Phase Response Evaluation in Milk

Nirala

Shtenberg

2020

Nanomaterials

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“…As an alternative direction for developing fluorescent based biosensors, Bracamonte and his group have developed highly fluorescent nanoparticles by utilizing GNPs as the core, silica as the shell (as a spacer), and rhodamine b (RhB) as a fluorophore for bacteria imaging and sensing ( Figure 3 C) [ 51 ]. The plasmon resonance maximum of the core–shell nanoparticles was able to be red shifted due to interactions with the GNP core based on the thickness of the silica shell.…”

Section: Gold Nanoparticle-based Plasmon-enhanced Fluorescence Andmentioning

confidence: 99%

Application of Gold Nanoparticle to Plasmonic Biosensors

Lee

Cho

Choi

et al. 2018

IJMS

134

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Gold nanoparticles (GNPs) have been widely utilized to develop various biosensors for molecular diagnosis, as they can be easily functionalized and exhibit unique optical properties explained by plasmonic effects. These unique optical properties of GNPs allow the expression of an intense color under light that can be tuned by altering their size, shape, composition, and coupling with other plasmonic nanoparticles. Additionally, they can also enhance other optical signals, such as fluorescence and Raman scattering, making them suitable for biosensor development. In this review, we provide a detailed discussion of the currently developed biosensors based on the aforementioned unique optical features of GNPs. Mainly, we focus on four different plasmonic biosensing methods, including localized surface plasmon resonance (LSPR), surface-enhanced Raman spectroscopy (SERS), fluorescence enhancement, and quenching caused by plasmon and colorimetry changes based on the coupling of GNPs. We believe that the topics discussed here are useful and able to provide a guideline in the development of novel GNP-based biosensors in the future.

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“…In this work, Rhodamine B was initially conjugated to (3-aminopropyl) trimethoxysilane (APS) using EDC/NHS coupling and then Rhodamine-APS was reacted with Ag@SiO 2 . 67 EDC/NHS coupling was also used to bind hemoglobin onto gold NPs entrapped in an aminated titanium dioxide shell. 68 This platform was used as a biosensor for the detection of hydrogen peroxide that is commonly used as a food additive and preservative.…”

Section: Amide Bond Formationmentioning

confidence: 99%

Surface modification of plasmonic noble metal–metal oxide core–shell nanoparticles

2019

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Synthesis of ultraluminescent gold core–shell nanoparticles as nanoimaging platforms for biosensing applications based on metal-enhanced fluorescence

Abstract: Core–shell nanoparticles are versatile nanostructures that can be applied as luminescent biosensing platforms in many nanotechnological developments.

Cited by 43 publications

References 26 publications

Amplified Fluorescence by ZnO Nanoparticles vs. Quantum Dots for Bovine Mastitis Acute Phase Response Evaluation in Milk

Amplified Fluorescence by ZnO Nanoparticles vs. Quantum Dots for Bovine Mastitis Acute Phase Response Evaluation in Milk

Application of Gold Nanoparticle to Plasmonic Biosensors

Surface modification of plasmonic noble metal–metal oxide core–shell nanoparticles

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