Synthesis of viral proteins in polymorphonuclear leukocytes infected with influenza A virus

Cassidy, L F; Lyles, Douglas S.; Abramson, J S

doi:10.1128/jcm.26.7.1267-1270.1988

Cited by 21 publications

(7 citation statements)

References 25 publications

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“…These data suggest that there is no productive infection of human neutrophils. These findings are at variance with a previous study by Cassidy et al (31), who reported that human neutrophils synthesize IAV proteins. It is possible that differences in the sensitivity of protein detection between the two methods-radiola- virus for up to 4 h. Association of virus with neutrophils was monitored by immunofluorescence microscopy.…”

Section: Resultscontrasting

confidence: 96%

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Differential Ability of Pandemic and Seasonal H1N1 Influenza A Viruses To Alter the Function of Human Neutrophils

Małachowa

Freedman

Sturdevant

et al. 2018

mSphere

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Neutrophils are essential cells of host innate immunity. Although the role of neutrophils in defense against bacterial and fungal infections is well characterized, there is a relative paucity of information about their role against viral infections. Influenza A virus (IAV) infection can be associated with secondary bacterial coinfection, and it has long been posited that the ability of IAV to alter normal neutrophil function predisposes individuals to secondary bacterial infections. To better understand this phenomenon, we evaluated the interaction of pandemic or seasonal H1N1 IAV with human neutrophils isolated from healthy persons. These viruses were ingested by human neutrophils and elicited changes in neutrophil gene expression that are consistent with an interferon-mediated immune response. The viability of neutrophils following coculture with either pandemic or seasonal H1N1 IAV was similar for up to 18 h of culture. Notably, neutrophil exposure to seasonal (but not pandemic) IAV primed these leukocytes for enhanced functions, including production of reactive oxygen species and bactericidal activity. Taken together, our results are at variance with the universal idea that IAV impairs neutrophil function directly to predispose individuals to secondary bacterial infections. Rather, we suggest that some strains of IAV prime neutrophils for enhanced bacterial clearance. A long-standing notion is that IAV inhibits normal neutrophil function and thereby predisposes individuals to secondary bacterial infections. Here we report that seasonal H1N1 IAV primes human neutrophils for enhanced killing of. Moreover, we provide a comprehensive view of the changes in neutrophil gene expression during interaction with seasonal or pandemic IAV and report how these changes relate to functions such as bactericidal activity. This study expands our knowledge of IAV interactions with human neutrophils.

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Section: Resultscontrasting

confidence: 96%

“…A more recent study by Zhang et al (32) suggests that there is a release of infectious IAV progeny from human neutrophils. In accordance with our current results, several other studies failed to provide compelling evidence that neutrophils produce infectious virus progeny, despite having the ability to amplify viral RNA (31,33,34).…”

Section: Resultssupporting

confidence: 92%

Differential Ability of Pandemic and Seasonal H1N1 Influenza A Viruses To Alter the Function of Human Neutrophils

Małachowa

Freedman

Sturdevant

et al. 2018

mSphere

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“…Concurrent measurement of viral RNA levels and infectivity led to the additional finding that, although human eosinophils infected with parainfluenza produce viral RNA, these viral progeny are not infectious. This phenomenon, termed an "abortive" infection, has been described for many viruses, including RSV (28) and coronavirus (29) in macrophages, and influenza A in neutrophils (30), as well as for parainfluenza virus in Madin-Darby bovine kidney cells (31) and chick embryo fibroblasts (32). Evidence suggests that specific virus and host cell characteristics impact productive versus abortive outcomes during virus infections (33), but those characteristics require further investigation.…”

Section: Eosmentioning

confidence: 96%

Human and Mouse Eosinophils Have Antiviral Activity against Parainfluenza Virus

Drake

Bivins-Smith

Proskocil

et al. 2016

Am J Respir Cell Mol Biol

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Respiratory viruses cause asthma exacerbations. Because eosinophils are the prominent leukocytes in the airways of 60-70% of patients with asthma, we evaluated the effects of eosinophils on a common respiratory virus, parainfluenza 1, in the lung. Eosinophils recruited to the airways of wild-type mice after ovalbumin sensitization and challenge significantly decreased parainfluenza virus RNA in the lungs 4 days after infection compared with nonsensitized animals. This antiviral effect was also seen in IL-5 transgenic mice with an abundance of airway eosinophils (NJ.1726) but was lost in transgenic eosinophil-deficient mice (PHIL) and in IL-5 transgenic mice crossed with eosinophil-deficient mice (NJ.1726-PHIL). Loss of the eosinophil granule protein eosinophil peroxidase, using eosinophil peroxidase-deficient transgenic mice, did not reduce eosinophils' antiviral effect. Eosinophil antiviral mechanisms were also explored in vitro. Isolated human eosinophils significantly reduced parainfluenza virus titers. This effect did not involve degradation of viral RNA by eosinophil granule RNases. However, eosinophils treated with a nitric oxide synthase inhibitor lost their antiviral activity, suggesting eosinophils attenuate viral infectivity through production of nitric oxide. Consequently, eosinophil nitric oxide production was measured with an intracellular fluorescent probe. Eosinophils produced nitric oxide in response to virus and to a synthetic agonist of the virus-sensing innate immune receptor, Toll-like receptor (TLR) 7. IFNγ increased expression of eosinophil TLR7 and potentiated TLR7-induced nitric oxide production. These results suggest that eosinophils promote viral clearance in the lung and contribute to innate immune responses against respiratory virus infections in humans.

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“…This prompted us to speculate the possibility of influenza virus multiplication in human neutrophils. A number of studies on the ability of viral replication in neutrophils were reported about 30 years ago, but none found any evidence of successful viral replication 1 2 13 . To our knowledge, this is the first report of infectious viral progeny production and release by neutrophils.…”

Section: Discussionmentioning

confidence: 99%

“…Traditionally, it was believed that influenza viral infection of neutrophils is abortive, and infectious progeny are not produced. There was no detectable level of viral progeny in the supernatant of cytomegalovirus, varicella-zoster virus or even the A/WSN/33 (H1N1) strain of influenza virus infected neutrophils in vitro 1 2 13 .…”

mentioning

confidence: 99%

Infectious Progeny of 2009 A (H1N1) Influenza Virus Replicated in and Released from Human Neutrophils

Zhang

Huang

et al. 2015

Sci Rep

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Various reports have indicated that a number of viruses could infect neutrophils, but the multiplication of viruses in neutrophils was abortive. Based on our previous finding that avian influenza viral RNA and proteins were present in the nucleus of infected human neutrophils in vivo, we investigated the possibility of 2009 A (H1N1) influenza viral synthesis in infected neutrophils and possible release of infectious progeny from host cells. In this study we found that human neutrophils in vitro without detectable level of sialic acid expression could be infected by this virus strain. We also show that the infected neutrophils can not only synthesize 2009 A (H1N1) viral mRNA and proteins, but also produce infectious progeny. These findings suggest that infectious progeny of 2009 A (H1N1) influenza virus could be replicated in and released from human neutrophils with possible clinical implications.

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Synthesis of viral proteins in polymorphonuclear leukocytes infected with influenza A virus

Cited by 21 publications

References 25 publications

Differential Ability of Pandemic and Seasonal H1N1 Influenza A Viruses To Alter the Function of Human Neutrophils

Differential Ability of Pandemic and Seasonal H1N1 Influenza A Viruses To Alter the Function of Human Neutrophils

Human and Mouse Eosinophils Have Antiviral Activity against Parainfluenza Virus

Infectious Progeny of 2009 A (H1N1) Influenza Virus Replicated in and Released from Human Neutrophils

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