Synthesis of α‐hydroxy stearyl coenzyme A

Chauhan, M. S.; Dakshinamurti, K.

doi:10.1007/bf02534077

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Enzymatic Water Elimination and Product Determination1

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1985

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“…The following retention times in min were observed: phenylalanine 1.9, phenyllactic acid 5.1, phenylpropionic acid 14.2, ^-cinnamic acid 25.0. The useof(2A)-[U 14 C]phenyllactate(0.25 · 10 6 dpm/Mmol) allowed the quantitative determination of all the products by analysis of aliquot parts in a liquid scintillation counter^1 8 !. When nonlabeled substrates were used, the determination of phenylpropionate and cinnamate was conducted by quantitative UV-absorption measurements.…”

Section: Enzymatic Water Elimination and Product Determinationmentioning

confidence: 99%

Observations on the Elimination of Water from 2-Hydroxy Acids in the Metabolism of Amino Acids byClostridium sporogenes

Machacek-Pitsch¹,

Rauschenbach²,

Simon³

1985

Biological Chemistry Hoppe-Seyler

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Cell-free extracts of Clostridium sporogenes catalyse the water elimination from (2/?)-phenyllactate in the presence of one of the energy-rich compounds acetyl-CoA, acetylphosphate or ATP and coenzyme A. Water is eliminated from (2/?)-phenyllactoyl-CoA without any of the aforementioned additions. CinnamoylCoA also acts catalytically. One molecule of cinnamoyl-CoA causes the elimination of water from more than 8 molecules phenyllactate. This is important from an energetic point of view since less than 2 mol ATP are formed per 2-3 mol metabolized amino acids. An activation of the hydroxy group of the -hydroxy acid in form of a phosphate ester can also be excluded for energetic reasons.

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Section: Enzymatic Water Elimination and Product Determinationmentioning

confidence: 99%