Synthesis of α2-antiplasmin by rat liver cells

Högstorp, Herman; Saldéen, T

doi:10.1016/0049-3848(82)90029-9

Cited by 12 publications

(3 citation statements)

References 16 publications

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“…Preliminary studies (11) have indicated that PB-AP is synthesized by the liver, in which case NPB-AP should be a metabolite, although the present findings do not preclude the reverse.…”

Section: -A P Was Increased In Rat Serum 24 H After Turpentine Trauma...contrasting

confidence: 77%

Two Forms of α₂-antiplasmin: Post-traumatic Changes in the Rat

Högstorp

Carlin

1987

Upsala Journal of Medical Sciences

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The plasminogen-binding (PB-AP) and non-plasminogen-binding (NPB-AP) forms of alpha 2-antiplasmin (AP), were assayed in rat plasma by a modified rocket immunoelectrophoretic technique before and up to 48 h after turpentine-induced trauma, using an intermediary gel containing kringles 1-3 from plasminogen. The concentration of PB-AP was significantly elevated by 22% 24 h post-traumatically, while NPB-AP was decreased at that point in time, leaving the total AP level unchanged. Total AP increased by 57% during the period 24-48 h after trauma, mainly on account of increases in the NPB-AP form. It is concluded that the plasma level of AP can remain unchanged in spite of increased fibrinolysis inhibition, owing to a relative increase in the functionally more active PB-AP.

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“…Preliminary studies (11) have indicated that PB-AP is synthesized by the liver, in which case NPB-AP should be a metabolite, although the present findings do not preclude the reverse.…”

Section: -A P Was Increased In Rat Serum 24 H After Turpentine Trauma...contrasting

confidence: 77%

Two Forms of α₂-antiplasmin: Post-traumatic Changes in the Rat

Högstorp

Carlin

1987

Upsala Journal of Medical Sciences

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“…A ∼55 residue long C-terminus (red) (Asn 410 -Lys 464 ; N 410 -K 464 ) of α2AP is important for initial binding with plasmin due to the presence of multiple lysine residues. α2AP EXPRESSION α2AP is primarily synthesized by hepatocytes in the liver and released into the blood (16,17). After synthesis, α2AP is enzymatically modified in the circulation at both the N-and C-terminus, which affects its fibrin-crosslinking and plasmin(ogen) binding capabilities respectively (18).…”

Section: Alpha2-antiplasmin (α2ap) Is the Serpin That Kills Plasminmentioning

confidence: 99%

Alpha2-Antiplasmin: The Devil You Don't Know in Cerebrovascular and Cardiovascular Disease

Singh

Saleem

Reed

2020

Front. Cardiovasc. Med.

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Alpha2-antiplasmin (α2AP), the fast-reacting, serine protease inhibitor (serpin) of plasmin, was originally thought to play a key role in protection against uncontrolled, plasmin-mediated proteolysis of coagulation factors and other molecules. However, studies of humans and mice with genetic deficiency of α2AP have expanded our understanding of this serpin, particularly in disease states. Epidemiology studies have shown an association between high α2AP levels and increased risk or poor outcome in cardiovascular diseases. Mechanistic studies in disease models indicate that α2AP stops the body's own fibrinolytic system from dissolving pathologic thrombi that cause venous thrombosis, pulmonary embolism, arterial thrombosis, and ischemic stroke. In addition, α2AP fosters the development of microvascular thrombosis and enhances matrix metalloproteinase-9 expression. Through these mechanisms and others, α2AP contributes to brain injury, hemorrhage and swelling in experimental ischemic stroke. Recent studies also show that α2AP is required for the development of stasis thrombosis by inhibiting the early activation of effective fibrinolysis. In this review, we will discuss the key role played by α2AP in controlling thrombosis and fibrinolysis and, we will consider its potential value as a therapeutic target in cardiovascular diseases and ischemic stroke.

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“…α 2 -AP is syn thesized by the liver. 42 It rapidly binds the plasmin molecule to form an inactive and stable complex. 78 As a consequence of α 2 -AP-plasmin complex for mation, both plasminogen and α 2 -AP levels decrease in plasma.…”

Section: Plasminogen and α 2 -Antiplasminmentioning

confidence: 99%