Synthesis of β‐tubulin occurs within chromatoid body of round spermatids

Fujii, Yuki; Fujita, Hideaki; Yokota, Sadaki

doi:10.1002/cm.21363

Cited by 5 publications

(3 citation statements)

References 32 publications

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“…Apart from that, BMAL1 might also directly affect the structure of sperm. The chromatoid body (CB) is a special structure in pachytene spermatocytes and spermatids, which plays a pivotal role in gametogenesis during meiosis and spermatogenesis (42)(43)(44). Of interest, BMAL1 depletion converted the CB into fragments in round spermatids (18), and BMAL1 expression declined with abnormal CB structures and functions with age (45).…”

Section: Effect Of Bmal1 Knockout On Male Reproduction In Micementioning

confidence: 99%

Critical Roles of the Circadian Transcription Factor BMAL1 in Reproductive Endocrinology and Fertility

Jiang

et al. 2022

Front. Endocrinol.

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Brain and muscle aryl-hydrocarbon receptor nuclear translocator like protein1 (BMAL1), a core component of circadian oscillation, is involved in many physiological activities. Increasing evidence has demonstrated the essential role of BMAL1 in reproductive physiology. For instance, BMAL1-knockout (KO) mice were infertile, with impaired reproductive organs and gametes. Additionally, in BMAL1-KO mice, hormone secretion and signaling of hypothalamus-pituitary-gonadal (H-P-G) hormones were also disrupted, indicating that H-P-G axis was impaired in BMAL1-KO mice. Moreover, both BMAL1-KO mice and BMAL1-knockdown by small interfering RNA (siRNA) in vitro cultured steroidogenic cells showed that BMAL1 was associated with gonadal steroidogenesis and expression of related genes. Importantly, BMAL1 also participates in pathogenesis of human reproductive diseases. In this review, we elaborate on the impaired reproduction of BMAL1-KO mice including the reproductive organs, reproductive endocrine hormones, and reproductive processes, highlighting the vital role of BMAL1 in fertility and reproductive endocrinology.

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Section: Effect Of Bmal1 Knockout On Male Reproduction In Micementioning

confidence: 99%

Critical Roles of the Circadian Transcription Factor BMAL1 in Reproductive Endocrinology and Fertility

Jiang

et al. 2022

Front. Endocrinol.

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“…ISPG, IMC and SB were observed in pachytene spermatocytes, while CB was observed in round sperms. In rat round sperm cells, Tubulin beta is translated from mRNA stored in CB, and assembled with Tubulin alpha outside CB to form a structural unit of microtubules: αβ-heterodimer, to construct microtubules in the sperm flella 30 .…”

Section: Experimental Results and Analysismentioning

confidence: 99%

Comparison of urinary proteome in the first two days after mating in male rats

Wang,

Zhao,

Gao

2023

Preprint

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ObjectiveTo compare urine proteome changes in the first two days after mating behavior in male rats.MethodsThe urine samples of Sprague-Dawley rats on the day of mating and the day after mating were collected and analyzed by non-label quantitative proteomics by high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS), and the differential proteins in the urine proteome were screened protein function and biological pathways were analyzed.Results43 differential proteins were identified by comparing the urine proteome of rats on the next day of mating with that on the day of mating, and nearly two-thirds of the differential proteins were related to spermatogenesis.ConclusionsThere were changes in the urinary proteome on the next day of mating compared with the day of mating, and some of the proteins whose functions were known to be related to spermatogenesis. Urine proteome has the potential to reflect spermatogenesis.

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“…S1B, left panel). β-Tubulin, one of the proteins participating in manchette assembly (Fujii et al, 2017;Lehti and Sironen, 2016), can be used as a marker of spermatid elongation. In CKO tubules, a skirt-like manchette structure was not observed (Fig.…”

Section: Resultsmentioning

confidence: 99%

Mouse EWSR1 is crucial for spermatid post-meiotic transcription and spermiogenesis

Tian

Petkov

2021

Development

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Spermatogenesis is precisely controlled by complex gene-expression programs. During mammalian male germ-cell development, a crucial feature is the repression of transcription before spermatid elongation. Previously, we discovered that the RNA-binding protein EWSR1 plays an important role in meiotic recombination in mouse, and showed that EWSR1 is highly expressed in late meiotic cells and post-meiotic cells. Here, we used an Ewsr1 pachytene stage-specific knockout mouse model to study the roles of Ewsr1 in late meiotic prophase I and in spermatozoa maturation. We show that loss of EWSR1 in late meiotic prophase I does not affect proper meiosis completion, but does result in defective spermatid elongation and chromocenter formation in the developing germ cells. As a result, male mice lacking EWSR1 after pachynema are sterile. We found that in Ewsr1 CKO round spermatids, transition from a meiotic gene-expression program to a post-meiotic and spermatid gene expression program related to DNA condensation is impaired, suggesting that EWSR1 plays an important role in regulation of spermiogenesis-related mRNA synthesis necessary for spermatid differentiation into mature sperm.

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Synthesis of β‐tubulin occurs within chromatoid body of round spermatids

Cited by 5 publications

References 32 publications

Critical Roles of the Circadian Transcription Factor BMAL1 in Reproductive Endocrinology and Fertility

Critical Roles of the Circadian Transcription Factor BMAL1 in Reproductive Endocrinology and Fertility

Comparison of urinary proteome in the first two days after mating in male rats

Mouse EWSR1 is crucial for spermatid post-meiotic transcription and spermiogenesis

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