Synthesis, pharmacokinetics, and biological use of lysine-modified single-walled carbon nanotubes

Feinberg, Evan N.; Alidori, Simone; McDevitt, Michael R.; Heller, Daniel A.; Scheinberg, David A.

doi:10.2147/ijn.s66050

Cited by 13 publications

(4 citation statements)

References 42 publications

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“…The binding of serum proteins with fCNT was examined using microscale thermophoresis to ask if the assembly of a protein corona surrounding fCNT is possible in vivo [ 17 ]. Binding isotherms for fCNT were generated ( S7 Fig ) and yielded K d values of 14 μM and 13 μM for serum albumin and IgG binding, respectively.…”

Section: Resultsmentioning

confidence: 99%

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Carbon nanotubes exhibit fibrillar pharmacology in primates

et al. 2017

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Nanomedicine rests at the nexus of medicine, bioengineering, and biology with great potential for improving health through innovation and development of new drugs and devices. Carbon nanotubes are an example of a fibrillar nanomaterial poised to translate into medical practice. The leading candidate material in this class is ammonium-functionalized carbon nanotubes (fCNT) that exhibits unexpected pharmacological behavior in vivo with important biotechnology applications. Here, we provide a multi-organ evaluation of the distribution, uptake and processing of fCNT in nonhuman primates using quantitative whole body positron emission tomography (PET), compartmental modeling of pharmacokinetic data, serum biomarkers and ex vivo pathology investigation. Kidney and liver are the two major organ systems that accumulate and excrete [86Y]fCNT in nonhuman primates and accumulation is cell specific as described by compartmental modeling analyses of the quantitative PET data. A serial two-compartment model explains renal processing of tracer-labeled fCNT; hepatic data fits a parallel two-compartment model. These modeling data also reveal significant elimination of the injected activity (>99.8%) from the primate within 3 days (t1/2 = 11.9 hours). These favorable results in nonhuman primates provide important insight to the fate of fCNT in vivo and pave the way to further engineering design considerations for sophisticated nanomedicines to aid late stage development and clinical use in man.

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Section: Resultsmentioning

confidence: 99%

“…HSA was serially diluted from 85.5 g/L to 0.0072 g/L; IgG was serially diluted from 18.5 g/L to 0.0016 g/L. The data were processed and plotted as described [ 17 ].…”

Section: Methodsmentioning

confidence: 99%

Carbon nanotubes exhibit fibrillar pharmacology in primates

et al. 2017

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“…Mulvay et al synthesized covalent amine-functionalized SWCNTs 1,3-cycloaddition using tert-butyloxycarbonyl (H-Lys-(Boc)-OH). Then, this conjugated CNT was bound to radiometalated chelator and exhibited good wrapping of siRNA [128]. …”

Section: Preparing Cnts For Drug Deliverymentioning

confidence: 99%

Carbon nanotubes part I: preparation of a novel and versatile drug-delivery vehicle

Karimi

Solati

Amiri

et al. 2015

Expert Opinion on Drug Delivery

102

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Introduction It is 23 years since carbon allotrope known as carbon nanotubes (CNT) was discovered by Iijima, who described them as “rolled graphite sheets inserted into each other”. Since then, CNTs have been studied in nanoelectronic devices. However, CNTs also possess the versatility to act as drug- and gene-delivery vehicles. Areas covered This review covers the synthesis, purification and functionalization of CNTs. Arc discharge, laser ablation and chemical vapor deposition are the principle synthesis methods. Non-covalent functionalization relies on attachment of biomolecules by coating the CNT with surfactants, synthetic polymers and biopolymers. Covalent functionalization often involves the initial introduction of carboxylic acids or amine groups, diazonium addition, 1,3-dipolar cycloaddition or reductive alkylation. The aim is to produce functional groups to attach the active cargo. Expert opinion In this review, the feasibility of CNT being used as a drug-delivery vehicle is explored. The molecular composition of CNT is extremely hydrophobic and highly aggregation-prone. Therefore, most of the efforts towards drug delivery has centered on chemical functionalization, which is usually divided in two categories; non-covalent and covalent. The biomedical applications of CNT are growing apace, and new drug-delivery technologies play a major role in these efforts.

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“…For example, they have demonstrated unique capabilities in fields ranging from nanoelectronics for creating highly functional integrated circuits to biomedicine as sensors of in vivo disease biomarkers [1,2]. Their low toxicity, large surface area, and ability to penetrate the cell membrane indicate that functionalized SWNTs can be effective agents to deliver exogenous biomolecules like proteins, DNA, and RNA directly into cells [3][4][5][6][7]. These nanoagents can be molecularly conjugated to specific ligands, such as folic acid, antibodies, and peptides, enabling targeted delivery of anticancer or antibacterial agents [8][9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Broadband Spectroscopic Thermoacoustic Characterization of Single-Walled Carbon Nanotubes

Bauer

Wang

Vollin

et al. 2015

Journal of Spectroscopy

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Carbon nanotubes have attracted interest as contrast agents for biomedical imaging because they strongly absorb electromagnetic radiation in the optical and microwave regions. This study applies thermoacoustic (TA) imaging and spectroscopy to measure the frequency-dependent absorption profile of single-walled carbon nanotubes (SWNT) in the ranges of 2.7–3.1 GHz and 7–9 GHz using two tunable microwave sources. Between 7 and 9 GHz, the peak TA signal for solutions containing semiconducting and metallic SWNTs increased monotonically with a slope of 1.75 AU/GHz (R2=0.95) and 2.8 AU/GHz (R2=0.93), respectively, relative to a water baseline. However, after compensating for the background signal from water, it was revealed that the TA signal from metallic SWNTs increased exponentially within this frequency band. Results suggest that TA imaging and spectroscopy could be a powerful tool for quantifying the absorption properties of SWNTs and optimizing their performance as contrast agents for imaging or heat sources for thermal therapy.

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Synthesis, pharmacokinetics, and biological use of lysine-modified single-walled carbon nanotubes

Cited by 13 publications

References 42 publications

Carbon nanotubes exhibit fibrillar pharmacology in primates

Carbon nanotubes exhibit fibrillar pharmacology in primates

Carbon nanotubes part I: preparation of a novel and versatile drug-delivery vehicle

Broadband Spectroscopic Thermoacoustic Characterization of Single-Walled Carbon Nanotubes

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