In this study, it was aimed to compare the properties of cotton fabrics finished by natural and sulfated β-cyclodextrin complexes of silver nanoparticles (AgNPs) for biomedical applications. For this aim, sulfated β-cyclodextrin (S-β-CD) was obtained from β-CD and they were applied to cotton fabrics with and without EDTA crosslinking agent. Then, all the fabrics were treated with AgNPs and inclusion complexes were formed. Within the scope of the study, antibacterial activity, washing stability, add-on, tensile strength, handle and color change of the samples were tested and compared to each other. In addition, SEM and EDX were performed on the samples to characterize the effects of finishing, FT-IR analysis was performed to characterize the chemical structures of β-CD and S-β-CD powders and XRD analysis was performed to characterize the AgNPs. As a result of the study, the treatment of S-β-CD complex with AgNPs and crosslinking this complex to cotton sample by means of EDTA was found to be the most favorable method.