Synthesis, secretion, and deposition of fibronectin in cultured human synovium

Lavietes, Beverly B.; Carsons, Steven E.; Diamond, Herbert S.; Laskin, Richard S.

doi:10.1002/art.1780280909

Cited by 26 publications

(13 citation statements)

References 26 publications

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“…Since fibronectin that is newly synthesized by metabolically labeled RA tissue explants is intact (230-240 kd) (26), these smaller peptides probably arise from proteolytic cleavage.…”

Section: Discussionmentioning

confidence: 99%

“…Passaged human synoviocytes exhibit fibroblastic morphology in culture (26), and fibroblast chemotactic activity of purified fibronectin ( Figure 9, panel A) has beed shown to be dependent on the cell-binding domain present on a 140-kd carboxy-terminal fragment (4). Rheumatoid synovial fluid fibronectin (190-220 kd) displayed chemotactic activity similar to that of intact plasma fibronectin, both mediating 200-400% of control migration at concentrations equivalent to 4.5 x lo-' moles of monomer ( Figure 9, panel D, lanes 1 and 2).…”

Section: Discussionmentioning

confidence: 99%

“…Fibronectin distribution is heterogeneous in rheumatoid synovium, and differences in local fibronectin concentration may modulate synoviocyte migration in the diseased tissue. The increased deposition that is seen in the proliferated lining and surrounding inflamed blood vessels (26,44) may mediate the recruitment of additional connective tissue cells and macrophages (14) to these areas.…”

Section: Discussionmentioning

confidence: 99%

“…Human synoviocytes derived from explants of rheumatoid synovium, obtained at the time of surgery, were maintained in culture as described (26). Early (the first through the third) passage cultures were used.…”

Section: Methodsmentioning

confidence: 99%

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The immunoreactivity, ligand, and cell binding characteristics of rheumatoid synovial fluid fibronectin

Carsons

Lavietes

Diamond

et al. 1985

Arthritis & Rheumatism

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Fibronectin promotes macrophage adherence and expression of Fc receptors, is chemotactic for fibroblasts, and is an opsonin for fibrin and denatured collagen. These properties suggest a role for fibronectin in the modulation of joint inflammation. Since structural modification of the fibronectin molecule has been shown to result in loss or de novo acquisition of opsonic and chemotactic activity, we determined the functional and immunochemical properties of fibronectin isolated from the inflamed joint. Eighty‐six percent of synovial fluids obtained from patients with active rheumatoid arthritis (RA) contained fibronectin fragments, and 39% of the fluids no longer displayed the dimeric form. Compared with native fibronectin, RA peptides were as active in promoting synoviocyte chemotaxis and in glycosaminoglycan binding, but displayed lower affinity for fibrin and gelatin. Although comparable with intact protein in augmenting monocyte attachment to gelatin, the RA synovial fluid peptides did not augment monocyte attachment to fibrin. Analysis of whole synovial fluid and isolated fibronectins by enzyme immunoassay showed that the increased fibronectin immunoreactivity, previously reported in RA synovial fluid, measures intact and nearly intact protein and does not measure extensively degraded fragments.

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“…Since fibronectin that is newly synthesized by metabolically labeled RA tissue explants is intact (230-240 kd) (26), these smaller peptides probably arise from proteolytic cleavage.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

The immunoreactivity, ligand, and cell binding characteristics of rheumatoid synovial fluid fibronectin

Carsons

Lavietes

Diamond

et al. 1985

Arthritis & Rheumatism

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“…Noevidence of synovitis was found macroscopically and histologically in the synovium in any of the patients. The synoviocytes were isolated by the explant culture technique of synovium (14,20). Adipose and fibrous tissues were removed from the collected tissue samples as muchas possible under a dissecting microscope.…”

Section: Methodsmentioning

confidence: 99%

Reconstruction Culture System Simulating Human Synovium: A Three-dimensional Collagen Gel Culture of Synoviocytes.

Taguchi

Hirano²,

Iwasaki³

et al. 1997

Cell Struct. Funct.

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ABSTRACT. Wedeveloped a reconstruction culture system simulating humansynovium to investigate the synoviocytes in a more physiological condition. This system with two types of dishes provided the two separated spaces corresponding to the joint cavity and nutrient vessels. The isolated normal synoviocytes were cultured in type-I collagen gel with a layer-seeded on top in a smaller inner dish with a porous bottom, which was placed in a larger outer dish. Weadded hyaluronic acid solution to the space on the gel to makean environmentclose to the physiological joint cavity. The space between the inner and outer dishes was filled with a complete medium to nourish the synoviocytes through the porous filter (Experiment 1). In addition, to examine cell-to-cell interaction, we created a co-culture model by mixing synoviocytes and peripheral blood mononuclear cells in the collagen gel matrix (Experiment 2). In this way we could reconstruct the synovium in vitro. The synoviocytes could survive and maintain their characteristics for four weeks of culture. In Experiment 1, almost all the cells were similar to type B synovial cells by histological, immunohistochemical and electron microscopic observations. In Experiment 2, the spherical cells in abundance of lysosomes like type A synovial cells appeared sporadically in the lining layer. Immunohistochemically, the majority of cultured cells expressed CD68and matrix metalloproteinase 3. This culture system promises to be of use in investigating the pathogenesis of various joint diseases.Humansynovium is comprised of lining and sub-lining layers with loose connective tissue. The lining layer consists of a few macrophage-like type A and manyfibroblast-like type B synovial cells (4, 5). These lining cells are nourished primarily via the blood circulation in the sub-lining layer (4), and are also influenced by joint fluid (1, 19). It has been pointed out, meanwhile, that extracellular matrix, e.g., collagen and fibronectin, in the sub-lining layers is involved in the regulation of the proliferation and function of synovial cells (1 1, 14, 17, 24). In the sub-lining layer, fibroblasts and a small number of mononuclear cells exist under physiological conditions, and in chronic synovitis the infiltrations of inflammatory cells are recognized histologically (4, 7, 10).The investigation of the complex interactions between synovial cells, inflammatory cells and joint fluid promises to contribute to the advance of the pathology and treatment in various joint diseases (7, 10, ll, 12, 16). A conventional culture system, using a monolayer culture of synovial fibroblasts, cannot offer a physiological condition of synovium described above. One of the authors (22) reported that a collagen gel culture system could provide an environment more similar to that in vivo. This system could reconstruct the structures of many types of tissues, i.e., skin, cornea, thyroid follicles, or pulmonary alveoli. However, as yet it has not been applied to the reconstruction of synovium. Wedeveloped a reconstruct...

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Fibronectin inhibits osteoclastogenesis while enhancing osteoclast activity via nitric oxide and interleukin‐1β‐mediated signaling pathways

Gramoun

Azizi

Sodek

et al. 2010

J of Cellular Biochemistry

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Osteoclasts are bone-resorbing cells formed by fusion of mononuclear precursors. The matrix proteins, fibronectin (FN), vitronectin (VN), and osteopontin (OPN) are implicated in joint destruction and interact with osteoclasts mainly through integrins. To assess the effects of these matrix proteins on osteoclast formation and activity, we used RAW 264.7 (RAW) cells and mouse splenocytes differentiated into osteoclasts on tissue culture polystyrene (TCP) or osteologic™ slides pre-coated with 0.01-20 µg/ml FN, VN, and OPN. At 96 h, osteoclast number and multinucleation were decreased on VN and FN compared to OPN and TCP in both RAW and splenocytes cell cultures. When early differentiation was assessed, VN but not FN decreased cytoplasmic tartrate-resistant acid phosphatase activity and pre-osteoclast number at 48 h. OPN had the opposite effect to FN on osteoclast formation. When RAW cells were differentiated on OPN and treated by FN and OPN, osteoclast number only in the FN treated group was 40-60% lower than the control, while the total number of nuclei was unchanged, suggesting that FN delays osteoclast fusion. In contrast to its inhibitory effect on osteoclastogenesis, FN increased resorption by increasing both osteoclast activity and the percentage of resorbing osteoclasts. This was accompanied by an increase in nitric oxide (NO) levels and interleukin-1β (IL-1β). IL-1β production was inhibited using the NO-synthase inhibitor only on FN indicating a FN-specific cross-talk between NO and IL-1β signaling pathways. We conclude that FN upregulates osteoclast activity despite inhibiting osteoclast formation and that these effects involve NO and IL-1β signaling.

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Synthesis, secretion, and deposition of fibronectin in cultured human synovium

Cited by 26 publications

References 26 publications

The immunoreactivity, ligand, and cell binding characteristics of rheumatoid synovial fluid fibronectin

The immunoreactivity, ligand, and cell binding characteristics of rheumatoid synovial fluid fibronectin

Reconstruction Culture System Simulating Human Synovium: A Three-dimensional Collagen Gel Culture of Synoviocytes.

Fibronectin inhibits osteoclastogenesis while enhancing osteoclast activity via nitric oxide and interleukin‐1β‐mediated signaling pathways

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