Synthesis, stability, cellular uptake, and blood circulation time of carboxymethyl-inulin coated magnetic nanoparticles

Abstract

Iron oxide nanoparticles were coated with the biocompatible, biodegradable, non-immunogenic polysaccharide inulin by introduction of carboxyl groups into the inulin structure and conjugation with amine groups on the surface of iron oxide nanoparticles grafted with 3-aminopropyltriethoxysilane. The resulting nanoparticles were characterized by FT-IR spectroscopy, transmission electron microscopy, dynamic light scattering, zeta potential, SQUID magnetometry, and with respect to their energy dissipation rate in applied alternating magnetic fields. The nanoparticles had a hydrodynamic diameter in the range of 70 ± 10 nm and were superparamagnetic, with energy dissipation rates in the range of 58–175 W/g for an applied field frequency of 233 kHz and an applied field amplitude in the range of 20–48 kA/m. The nanoparticles were stable in a range of pH, at temperatures between 23°C and 53°C, and in short term storage in water, PBS, and culture media. The particles were non-cytotoxic to the immortalized human cancer cell lines Hey A8 FDR, A2780, MDA 468, MCF-7 and Caco-2. The nanoparticles were readily taken up by Caco-2 cells in a time and concentration dependent fashion, and were found to have a pharmacokinetic time constant of 47 ± 3 min. The small size, non-cytotoxicity, and efficient energy dissipation of the particles could make them useful for biomedical applications such as magnetic fluid hyperthermia.

Chemical and Colloidal Stability of Carboxylated Core-Shell Magnetite Nanoparticles Designed for Biomedical Applications

“…We did not attempt measuring desorption of CA and GA because their adsorption is superimposed by surface polymerization making desorption experiments irrelevant. It has been shown for a series of core-shell MNPs [31,32] that shells can be displaced by adsorption of phosphate from biological buffers depending on the size and the anchoring type of coating molecule and the density of coating. Samples resisting shell detachment in aqueous NaCl solutions should be subject to tests in PBS and different cell culture media in further in vitro studies.…”

Nanoantioxidants: Recent Trends in Antioxidant Delivery Applications

“…However, the tailoring of Fe 2 O 3 NPs via different strategies including coating with carbon [55], carboxymethyl-inulin [56], and poly(GA) [57], surface functionalization with natural antioxidant (GA) [27], as well as curcumin in magnetic-silk core-shell nanoparticles [58], was successfully accomplished. These tailored Fe 2 O 3 NP composites showed better dispersibility and stability and were evaluated for the efficient antioxidant properties, antimicrobial activities, and targeted drug delivery to the specific organs, as well as being analyzed for their cytotoxicity and biocompatibility/hemocompatibility [27,[55][56][57][58][59]. Surface-functionalized Fe 2 O 3 NPs with GA by in situ and post-synthesis with an average particle size of 5 and 8 nm, respectively, exhibited a 2-4 fold enhanced IC50 values of DPPH antioxidant assay compared to nonfunctionalized Fe 2 O 3 NPs.…”

Why Does Inulin have Pharmacological Activity? Theoretical Research by DFT and Concept DFT Methods

“…The bond angle near the C 2 is not 120° and deflect from sp 2 is due to the group electron attraction of oxygen atom in e molecule. In all the error between molecular structure data and experimental parameter [31][32][33] is minimum, so their molecular structure is credible.…”