In this work, Zn1-xNixO NPs with different concentration (x= 0.0, 0.03, 0.06 and 0.09) were synthesized by using co-precipitation method. The present study is related to investigate crystal structure, surface morphology, optical and magnetic behavior of pure and Ni dopant ZnO NPs. The hexagonal wurtzite structure and crystallite size (19.45 to 21.25 nm) was investigated by using XRD analysis.SEM and FTIR used to identify the surface morphology, stretching and vibrational modes of different functional groups (ZnO, OH, CO and H-O-H) attached on the spectrum of Ni dopant ZnO NPs. Moreover, the optical behavior of Ni dopant ZnO NPs was studied by UV-visible spectroscopy which indicates the absorption band of red shift. Finally, VSM analysis illustrated that Ni dopant ZnO NPs shows high magnetization, retentivity and low corecivity as compare to pure ZnO NPs. In an overall assessment the Ni dopant ZnO NPs shows better optical and magnetic properties for biomedical applications.