Synthesis Variants of Quaternary Ammonium Polyethyleneimine Nanoparticles and Their Antibacterial Efficacy in Dental Materials

Zaltsman, Nathan; Kesler-Shvero, Dana; Weiss, Ervin I.; Beyth, Nurit

doi:10.5301/jabfm.5000269

Cited by 18 publications

(27 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…QPEI NPs were synthesized as previously described [ 11 ]. Briefly, 600,000–1,000,000 Da polyethyleneimine (Sigma-Aldrich) were lyophilized (10.1 g, 0.232 mol) and then dissolved in 100 mL absolute ethanol.…”

Section: Methodsmentioning

confidence: 99%

“…The QPEI NPs were rinsed again with DDW, lyophilized and pulverized to a fine powder. Infrared spectroscopy was used to benchmark the prepared QPEI NPs with those reported previously in the literature [ 11 ] ( Fig 1c ).…”

Section: Methodsmentioning

confidence: 99%

“…Aside from the antibacterial monomers added to the resin matrix, antibacterial solid nanoparticles (NPs) with QAS functionality were proposed. Quaternary ammonium polyethyleneimine (QPEI) nanoparticles were found to be contact-active bactericides when incorporated in various resin-based materials [ 3 – 11 ]. These are macromoleculular nanoparticles, i.e.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Surface-modified nanoparticles as anti-biofilm filler for dental polymers

et al. 2017

Self Cite

View full text Add to dashboard Cite

The objective of the study was to synthesis silica nanoparticles modified with (i) a tertiary amine bearing two t-cinnamaldehyde substituents or (ii) dimethyl-octyl ammonium, alongside the well-studied quaternary ammonium polyethyleneimine nanoparticles. These were to be evaluated for their chemical and mechanical properties, as well for antibacterial and antibiofilm activity. Samples were incorporated in commercial dental resin material and the degree of monomer conversion, mechanical strength, and water contact angle were tested to characterize the effect of the nanoparticles on resin material. Antibacterial activity was evaluated with the direct contact test and the biofilm inhibition test against Streptococcus mutans. Addition of cinnamaldehyde-modified particles preserved the degree of conversion and compressive strength of the base material and increased surface hydrophobicity. Quaternary ammonium functional groups led to a decrease in the degree of conversion and to low compressive strength, without altering the hydrophilic nature of the base material. In the direct contact test and the anti-biofilm test, the polyethyleneimine particles exhibited the strongest antibacterial effect. The cinnamaldehyde-modified particles displayed antibiofilm activity, silica particles with quaternary ammonium were ineffective. Immobilization of t-cinnamaldehyde onto a solid surface via amine linkers provided a better alternative to the well-known quaternary ammonium bactericides.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Surface-modified nanoparticles as anti-biofilm filler for dental polymers

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…To improve the antibacterial properties or to pioneer new application fields of these antibiotics, numerous studies have been reported. However, most of them focused on synthesis of new antibacterial organic molecules (Kourai et al 2006;Chanawanno et al 2010;Shtyrlin et al 2016) or polymerization with R4N + (Jiao et al 2017;Farah et al 2015;Zaltsman et al 2016). That is, there is few report with respect to composites between metal or inorganic materials and antibiotic molecules.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of antibacterial effect of quaternary ammonium with inorganic nanosheets against Enterobacter cloacae

et al. 2018

View full text Add to dashboard Cite

To suppress nosocomial infections, numerous studies of quaternary ammonium cations (R4N +) to improve the antibiotic properties have been investigated. However, most of them reported developments of novel organic or polymeric materials with R4N +. To pioneer antibacterial inorganic materials hybridized with R4N + , a colloidal solution of metal oxide nanosheets, which have a small particle size (typically less than 10 nm), is considered to be a suitable option because oxide nanosheets with a negative surface charge strongly interact R4N +. Herein, we demonstrate for the first time that the high antibacterial/bactericidal effects of titanate nanosheets (TNS) adsorbing tetramethylammonium (TMA-TNS) or tetrabultylammonium ions (TBA-TNS). Their antibacterial effects against Enterobacter cloacae were evaluated using a colony forming unit (CFU) counting method. The results showed that the synthesized TNS composites had superior antibacterial and bactericidal effects to those of free R4N + and TBA-TNS exhibited the strongest effect (69% CFU reduction compared with that of free TBA + and 98% CFU reduction compared with the control) among the samples examined. Dark incubation was employed to ensure photocatalytic reaction of semiconducting TNS did not contribute to the process. Compared with TiO2 spherical particles, such high bactericidal effect would be induced by a synergistic function of TBA + and TNS, which physically damages bacteria due to long hydrophobic alkyl chains and an anisotropic nanocrystalline structure with sharp edges, respectively.

show abstract

“…QAs are cationic surfactants with a broad spectrum of antibacterial activities. 7 – 8 Their antibacterial mechanisms are not well known, but it is generally accepted that the predominant mode of action is “contact killing”. The positively charged (N + ) sites of QA molecules can attach to the negatively charged bacterial cells.…”

Section: Introductionmentioning

confidence: 99%

Quaternary ammonium-induced multidrug tolerant Streptococcus mutans persisters elevate cariogenic virulence in vitro

et al. 2017

View full text Add to dashboard Cite

Dental caries are the most prevalent chronic infections in the oral cavity, and Streptococcus mutans acts as the main cariogenic bacterial species. Antibacterial quaternary ammonium compounds (QAs) have been developed to preveFnt or treat dental caries. However, there is no report on the tolerance of S. mutans to QAs. In this study, we investigated the development of S. mutans persistence induced by a novel dental caries defensive agent, dimethylaminododecyl methacrylate (DMADDM), for the first time. Typical biphasic killing kinetics for persisters were observed in both S. mutans planktonic and biofilm cultures challenged by DMADDM at concentrations of 20 and 200 μg·mL−1, respectively. The persisters tolerated six other antibiotics with different antibacterial mechanisms, while only daptomycin and vancomycin could slightly reduce the persister numbers in planktonic cultures. The distribution of persisters in DMADDM-treated biofilms was similar to that in the untreated control, except that the total biomass and biofilm height were significantly reduced. A higher exopolysaccharides (EPS):bacteria ratio was observed in DMADDM-treated biofilms. Persisters in biofilms significantly upregulated gtf gene expression, indicating an increase in the bacteria’s ability to produce EPS and an elevated capability of cariogenic virulence. Carbon source metabolism was significantly reduced, as related metabolic genes were all downregulated in persisters. Concentrations of 0.1 mM, 1 mM and 10 mM of extra glucose significantly reduced the number of persisters both in planktonic and biofilm conditions. The formation of non-inheritable and multidrug tolerant persisters induced by DMADDM suggested that drug tolerance and new persistent eradication strategies should be considered for oral antibacterial agents.

show abstract

Synthesis Variants of Quaternary Ammonium Polyethyleneimine Nanoparticles and Their Antibacterial Efficacy in Dental Materials

Abstract: The antimicrobial efficacy of QPEI NP may be improved significantly by controlling the addition of NaHCO3, neutralization of excess base and the surface-agent effect.

Cited by 18 publications

References 24 publications

Surface-modified nanoparticles as anti-biofilm filler for dental polymers

Surface-modified nanoparticles as anti-biofilm filler for dental polymers

Enhancement of antibacterial effect of quaternary ammonium with inorganic nanosheets against Enterobacter cloacae

Quaternary ammonium-induced multidrug tolerant Streptococcus mutans persisters elevate cariogenic virulence in vitro

Contact Info

Product

Resources

About