Synthetic antimicrobial peptides: From choice of the best sequences to action mechanisms

Souza, Pedro Filho Noronha; Marques, Lidyane S.M.; Oliveira, José T.A.; Lima, Patrícia G.; Dias, Lucas Pinheiro; Neto, Nilton A. S.; Lopes, Francisco E.S.; Sousa, J. S. de; Silva, Ayrles F.B.; Caneiro, Rômulo F.; Lopes, José Luiz de Souza; Ramos, Márcio V.; Freitas, Cléverson D.T.

doi:10.1016/j.biochi.2020.05.016

Cited by 92 publications

(77 citation statements)

References 51 publications

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“…The 3D structures of Rc Alb-PepI, Rc Alb-PepII, Rc Alb-PepIII were the same as employed by Dias et al [ 24 ]. The 3D structures of PepGAT and PepKAA were the same as employed by Souza et al [ 25 ]. The 3D structure files of SARS-CoV-2 Spike protein (open state, PDB: 6VYB ; closed state, PDB: 6VXX ) and human ACE2 (ACE2; PDB: 1R42 ) were downloaded from Protein Data Bank (PDB, https://www.rcsb.org/ ).…”

Section: Methodsmentioning

confidence: 99%

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A molecular docking study revealed that synthetic peptides induced conformational changes in the structure of SARS-CoV-2 spike glycoprotein, disrupting the interaction with human ACE2 receptor

Souza

Lopes

Amaral

et al. 2020

International Journal of Biological Macromolecules

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The global outbreak of COVID-19 (Coronavirus Disease 2019) caused by SARS-CoV-2 (Severe Acute Respiratory Syndrome caused by Coronavirus 2) began in December 2019. Its closest relative, SARS-CoV-1, has a slightly mutated Spike (S) protein, which interacts with ACE2 receptor in human cells to start the infection. So far, there are no vaccines or drugs to treat COVID-19. So, research groups worldwide are seeking new molecules targeting the S protein to prevent infection by SARS-CoV-2 and COVID-19 establishment. We performed molecular docking analysis of eight synthetic peptides against SARS-CoV-2 S protein. All interacted with the protein, but Mo-CBP 3-PepII and PepKAA had the highest affinity with it. By binding to the S protein, both peptides led to conformational alterations in the protein, resulting in incorrect interaction with ACE2. Therefore, given the importance of the S protein-ACE2 interaction for SARS-CoV-2 infection, synthetic peptides could block SARS-CoV-2 infection. Moreover, unlike other antiviral drugs, peptides have no toxicity to human cells. Thus, these peptides are potential molecules to be tested against SARS-CoV-2 and to develop new drugs to treat COVID-19.

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Section: Methodsmentioning

confidence: 99%

“…Here we report a MD study using eight antimicrobial peptides (Mo-CBP 3 -PepI, Mo-CBP 3 -PepII, Mo-CBP 3 -PepIII [22,23], RcAlb-PepI, RcAlb-PepII, RcAlb-PepIII [24], PepGAT and PepKAA [25] (unpublished data)) that target the SARS-CoV-2 S protein. With different affinities, all peptides interact with the RBD from the SARS-CoV-2 S protein.…”

Section: Introductionmentioning

confidence: 99%

A molecular docking study revealed that synthetic peptides induced conformational changes in the structure of SARS-CoV-2 spike glycoprotein, disrupting the interaction with human ACE2 receptor

Souza

Lopes

Amaral

et al. 2020

International Journal of Biological Macromolecules

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“…Before hydrolysis, the source of peptides may be subjected to various processes, e.g., fermentation [ 9 , 10 , 11 ]. Biopeptides can also be synthesized chemically [ 12 , 13 , 14 ] or can be obtained through the expression of appropriate genes [ 15 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Current Trends of Bioactive Peptides—New Sources and Therapeutic Effect

Jakubczyk

Karaś

Rybczyńska‐Tkaczyk

et al. 2020

Foods

164

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Generally, bioactive peptides are natural compounds of food or part of protein that are inactive in the precursor molecule. However, they may be active after hydrolysis and can be transported to the active site. Biologically active peptides can also be synthesized chemically and characterized. Peptides have many properties, including antihypertensive, antioxidant, antimicrobial, anticoagulant, and chelating effects. They are also responsible for the taste of food or for the inhibition of enzymes involved in the development of diseases. The scientific literature has described many peptides with bioactive properties obtained from different sources. Information about the structure, origin, and properties of peptides can also be found in many databases. This review will describe peptides inhibiting the development of current diseases, peptides with antimicrobial properties, and new alternative sources of peptides based on the current knowledge and documentation of their bioactivity. All these issues are part of modern research on peptides and their use in current health or technological problems in food production.

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“…As a result of this bioactivity, the antimicrobial proteins and/or peptides from plants have gained attention as an alternative treatment for parasite infection. However, three problems arise: (1) high cost for the purification process; (2) low resistance to proteolysis; and (3) toxicity to host hidden practical applications [14]. As a solution for that, synthetic peptides have come out.…”

Section: Introductionmentioning

confidence: 99%

New Insights into Anthelmintic Mechanisms of Action of a Synthetic Peptide: An Ultrastructural and Nanomechanical Approach

et al. 2021

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Resistant nematodes are not affected by the most common drugs commercially available. In the search for new anthelmintics, peptides have been investigated. Here, a linear synthetic peptide named RcAlb-PepIII bioinspired from the antimicrobial protein Rc-2S-Alb was designed, synthesized, and tested against barber pole worm Haemonchus contortus. The physicochemical properties of the peptide, the 3D structure model, the egg hatch inhibition, and larval development inhibition of H. contortus were carried out. Additionally, the ultrastructure of the nematode after treatment with the peptide was evaluated by atomic force microscopy. The RcAlb-PepIII inhibited the larval development of H. contortus with an EC50 of 90 µM and did not affect egg hatch. Atomic force microscopy reveals the high affinity of RcAlb-PepIII with the cuticle of H. contortus in the L2 stage. It also shows the deposition of RcAlb-PepIII onto the surface of the cuticle, forming a structure similar to a film that reduces the roughness and mean square roughness (Rq) of it. In conclusion, the bioinspired RcAlb-PepIII has the potential to be used as a new anthelmintic compound to control gastrointestinal nematode parasites.

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Synthetic antimicrobial peptides: From choice of the best sequences to action mechanisms

Cited by 92 publications

References 51 publications

A molecular docking study revealed that synthetic peptides induced conformational changes in the structure of SARS-CoV-2 spike glycoprotein, disrupting the interaction with human ACE2 receptor

A molecular docking study revealed that synthetic peptides induced conformational changes in the structure of SARS-CoV-2 spike glycoprotein, disrupting the interaction with human ACE2 receptor

Current Trends of Bioactive Peptides—New Sources and Therapeutic Effect

New Insights into Anthelmintic Mechanisms of Action of a Synthetic Peptide: An Ultrastructural and Nanomechanical Approach

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