Synthetic biology for CO2 fixation

Gong, Fuyu; Zhang, Cai; Li, Yin

doi:10.1007/s11427-016-0304-2

Cited by 65 publications

(32 citation statements)

References 48 publications

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“…A key barrier to this process is the CO 2 emissions that occur during natural aerobic fermentation. Many carbon dioxide fixation pathways have been exploited using the six carbon fixation pathways discovered in nature ( Gong et al, 2016 ). However, complex reaction steps and enzyme requirements limit the broad application of these carbon sequestration pathways ( Erb et al, 2007 ; Schwander et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Enhancing the Glucose Flux of an Engineered EP-Bifido Pathway for High Poly(Hydroxybutyrate) Yield Production

Liu

Sun

et al. 2020

Front. Bioeng. Biotechnol.

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Background As the greenhouse effect becomes more serious and carbon dioxide emissions continue rise, the application prospects of carbon sequestration or carbon-saving pathways increase. Previously, we constructed an EP-bifido pathway in Escherichia coli by combining Embden-Meyerhof-Parnas pathway, pentose phosphate pathway and “bifid shunt” for high acetyl-CoA production. There is much room for improvement in the EP-bifido pathway, including in production of target compounds such as poly(hydroxybutyrate) (PHB). Result To optimize the EP-bifido pathway and obtain higher PHB yields, we knocked out the specific phosphoenolpyruvate phosphate transferase system (PTS) component II Cglc, encoded by ptsG . This severely inhibited the growth and sugar consumption of the bacterial cells. Subsequently, we used multiple automated genome engineering (MAGE) to optimize the ribosome binding site (RBS) sequences of galP (galactose: H (+) symporter) and glk (glucokinase gene bank: NC_017262.1), encoding galactose permease and glucokinase, respectively. Growth and glucose uptake were partially restored in the bacteria. Finally, we introduced the glf (UDP-galactopyranose) from Zymomonas mobilis mutase sugar transport vector into the host strain genome. Conclusion After optimizing RBS of galP , the resulting strain L-6 obtained a PHB yield of 71.9% (mol/mol) and a 76 wt% PHB content using glucose as the carbon source. Then when glf was integrated into the genome strain L-6, the resulting strain M-6 reached a 5.81 g/L PHB titer and 85.1 wt% PHB content.

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Section: Introductionmentioning

confidence: 99%

Enhancing the Glucose Flux of an Engineered EP-Bifido Pathway for High Poly(Hydroxybutyrate) Yield Production

Liu

Sun

et al. 2020

Front. Bioeng. Biotechnol.

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“…Geothermal ecosystems are low in organic carbon, but CO 2 is highly available and can be assimilated into biomass by chemolithoautotrophic microorganisms. In total, six CO 2 fixation pathways have been discovered, namely, the Calvin-Benson-Bassham (CBB) cycle, the 3-hydroxypropionate cycle, the 3-hydroxypropionate-4-hydroxybutyrate cycle, the reductive tricarboxylic acid (TCA) cycle, the Wood-Ljungdahl pathway, and the dicarboxylate/4-hydroxybutyrate cycle (35,36). Fixing inorganic carbon requires energy and oxidation of the reduced geothermal gases can provide this energy.…”

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confidence: 99%

Geothermal Gases Shape the Microbial Community of the Volcanic Soil of Pantelleria, Italy

et al. 2020

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Volcanic and geothermal environments are characterized by low pH, high temperatures, and gas emissions consisting of mainly CO2 and varied CH4, H2S, and H2 contents which allow the formation of chemolithoautotrophic microbial communities. To determine the link between the emitted gases and the microbial community composition, geochemical and metagenomic analysis were performed. Soil samples of the geothermic region Favara Grande (Pantelleria, Italy) were taken at various depths (1 to 50 cm). Analysis of the gas composition revealed that CH4 and H2 have the potential to serve as the driving forces for the microbial community. Our metagenomic analysis revealed a high relative abundance of Bacteria in the top layer (1 to 10 cm), but the relative abundance of Archaea increased with depth from 32% to 70%. In particular, a putative hydrogenotrophic methanogenic archaeon, related to Methanocella conradii, appeared to have a high relative abundance (63%) in deeper layers. A variety of [NiFe]-hydrogenase genes were detected, showing that H2 was an important electron donor for microaerobic microorganisms in the upper layers. Furthermore, the bacterial population included verrucomicrobial and proteobacterial methanotrophs, the former showing an up to 7.8 times higher relative abundance. Analysis of the metabolic potential of this microbial community showed a clear capacity to oxidize CH4 aerobically, as several genes for distinct particulate methane monooxygenases and lanthanide-dependent methanol dehydrogenases (XoxF-type) were retrieved. Analysis of the CO2 fixation pathways showed the presence of the Calvin-Benson-Bassham cycle, the Wood-Ljungdahl pathway, and the (reverse) tricarboxylic acid (TCA) cycle, the latter being the most represented carbon fixation pathway. This study indicates that the methane emissions in the Favara Grande might be a combination of geothermal activity and biological processes and further provides insights into the diversity of the microbial population thriving on CH4 and H2. IMPORTANCE The Favara Grande nature reserve on the volcanic island of Pantelleria (Italy) is known for its geothermal gas emissions and high soil temperatures. These volcanic soil ecosystems represent “hot spots” of greenhouse gas emissions. The unique community might be shaped by the hostile conditions in the ecosystem, and it is involved in the cycling of elements such as carbon, hydrogen, sulfur, and nitrogen. Our metagenome study revealed that most of the microorganisms in this extreme environment are only distantly related to cultivated bacteria. The results obtained profoundly increased the understanding of these natural hot spots of greenhouse gas production/degradation and will help to enrich and isolate the microbial key players. After isolation, it will become possible to unravel the molecular mechanisms by which they adapt to extreme (thermo/acidophilic) conditions, and this may lead to new green enzymatic catalysts and technologies for industry.

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“…Several definitive studies on carbon fixation by frog embryos were performed by Nobel laureate Stanley Cohen (1954 , 1963) , who later went on to discover nerve and epidermal growth factors ( Shampo, 1999 ). Interestingly, the study of heterotrophic carbon fixation in amphibian embryos has received little research attention since the 1970’s despite decades of biochemical and molecular research into the mechanisms of development ( Elinson and del Pino, 2011 ) and parallel research attention into the mechanisms of carbon fixation ( Gong et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Heterotrophic Carbon Fixation in a Salamander-Alga Symbiosis

2020

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The unique symbiosis between a vertebrate salamander, Ambystoma maculatum, and unicellular green alga, Oophila amblystomatis, involves multiple modes of interaction. These include an ectosymbiotic interaction where the alga colonizes the egg capsule, and an intracellular interaction where the alga enters tissues and cells of the salamander. One common interaction in mutualist photosymbioses is the transfer of photosynthate from the algal symbiont to the host animal. In the A. maculatum-O. amblystomatis interaction, there is conflicting evidence regarding whether the algae in the egg capsule transfer chemical energy captured during photosynthesis to the developing salamander embryo. In experiments where we took care to separate the carbon fixation contributions of the salamander embryo and algal symbionts, we show that inorganic carbon fixed by A. maculatum embryos reaches 2% of the inorganic carbon fixed by O. amblystomatis algae within an egg capsule after 2 h in the light. After 2 h in the dark, inorganic carbon fixed by A. maculatum embryos is 800% of the carbon fixed by O. amblystomatis algae within an egg capsule. Using photosynthesis inhibitors, we show that A. maculatum embryos and O. amblystomatis algae compete for available inorganic carbon within the egg capsule environment. Our results confirm earlier studies suggesting a role of heterotrophic carbon fixation during vertebrate embryonic development. Our results also show that the considerable capacity of developing A. maculatum embryos for inorganic carbon fixation precludes our ability to distinguish any minor role of photosynthetically transferred carbon from algal symbionts to host salamanders using bicarbonate introduced to the egg system as a marker.

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Synthetic biology for CO2 fixation

Cited by 65 publications

References 48 publications

Enhancing the Glucose Flux of an Engineered EP-Bifido Pathway for High Poly(Hydroxybutyrate) Yield Production

Enhancing the Glucose Flux of an Engineered EP-Bifido Pathway for High Poly(Hydroxybutyrate) Yield Production

Geothermal Gases Shape the Microbial Community of the Volcanic Soil of Pantelleria, Italy

Heterotrophic Carbon Fixation in a Salamander-Alga Symbiosis

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