Synthetic chromosomes, genomes, viruses, and cells

Venter, J. Craig; Glass, John I.; Hutchison, Clyde A.; Vashee, Sanjay

doi:10.1016/j.cell.2022.06.046

Cited by 80 publications

(46 citation statements)

References 102 publications

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“…Viruses can be rescued through chemical DNA synthesis of authentic or recoded genomic sequences ( Venter et al., 2022 ). SynViP, a synthetic genomics framework with plant virome capacity, was recently conceived for one-step, seamless virus clone assembly using chemically synthesized DNA.…”

Section: Before You Beginmentioning

confidence: 99%

Assembly of plant virus agroinfectious clones using biological material or DNA synthesis

Pasin¹

2022

STAR Protocols

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Section: Before You Beginmentioning

confidence: 99%

Assembly of plant virus agroinfectious clones using biological material or DNA synthesis

Pasin¹

2022

STAR Protocols

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“…The J. Craig Venter team presented a multistep procedure to assemble the entire Mycoplasma genitalium genome. This development was further enhanced by Synthetic Genomics Inc., a company that focuses on the study and commercialization of specifically constructed genomes [ 212 ]. Synthetic genomics, which includes novel protein designs, structural analysis, and the production of recombinant vaccines for quickly evolving microbial infections, has the potential to accelerate vaccine development.…”

Section: Synthetic Genomics: the Future Of Vaccine Developmentmentioning

confidence: 99%

Recent Advances in Genomics-Based Approaches for the Development of Intracellular Bacterial Pathogen Vaccines

et al. 2022

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Infectious diseases continue to be a leading cause of morbidity and mortality worldwide. The majority of infectious diseases are caused by intracellular pathogenic bacteria (IPB). Historically, conventional vaccination drives have helped control the pathogenesis of intracellular bacteria and the emergence of antimicrobial resistance, saving millions of lives. However, in light of various limitations, many diseases that involve IPB still do not have adequate vaccines. In response to increasing demand for novel vaccine development strategies, a new area of vaccine research emerged following the advent of genomics technology, which changed the paradigm of vaccine development by utilizing the complete genomic data of microorganisms against them. It became possible to identify genes related to disease virulence, genetic patterns linked to disease virulence, as well as the genetic components that supported immunity and favorable vaccine responses. Complete genomic databases, and advancements in transcriptomics, metabolomics, structural genomics, proteomics, immunomics, pan-genomics, synthetic genomics, and population biology have allowed researchers to identify potential vaccine candidates and predict their effects in patients. New vaccines have been created against diseases for which previously there were no vaccines available, and existing vaccines have been improved. This review highlights the key issues and explores the evolution of vaccines. The increasing volume of IPB genomic data, and their application in novel genome-based techniques for vaccine development, were also examined, along with their characteristics, and the opportunities and obstacles involved. Critically, the application of genomics technology has helped researchers rapidly select and evaluate candidate antigens. Novel vaccines capable of addressing the limitations associated with conventional vaccines have been developed and pressing healthcare issues are being addressed.

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“…Many have suggested that synthetic biology approaches could be used to meet this challenge (e.g. ( 2 – 4 )). In bacteria and yeast, it is possible to chemically synthesize megabase-scale sequences and shuttle them between species to alter gene order and structure ( 4 – 7 ).…”

Section: Introductionmentioning

confidence: 99%

“…( 2 – 4 )). In bacteria and yeast, it is possible to chemically synthesize megabase-scale sequences and shuttle them between species to alter gene order and structure ( 4 – 7 ). Similar methods have been used to move DNA from yeast into human cells ( 4 ) and could be adapted to plants as well ( 8 ).…”

Section: Introductionmentioning

confidence: 99%

“…In bacteria and yeast, it is possible to chemically synthesize megabase-scale sequences and shuttle them between species to alter gene order and structure ( 4 – 7 ). Similar methods have been used to move DNA from yeast into human cells ( 4 ) and could be adapted to plants as well ( 8 ). Engineering small chromosomes for practical applications in medicine and agriculture seems a reasonable near-term goal ( 8 – 10 ).…”

Section: Introductionmentioning

confidence: 99%

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Synthetic maize centromeres transmit chromosomes across generations

Dawe

Gent

Zeng

et al. 2022

Preprint

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Centromeres are long, often repetitive regions of genomes that bind kinetochore proteins and ensure normal chromosome segregation. Engineering centromeres that function in vivo has proven to be difficult. Here we describe a LexA-CENH3 tethering approach that activates functional centromeres at maize synthetic repeat arrays containing LexO binding sites. The synthetic centromeres are sufficient to cause chromosome breakage and release of chromosome fragments that are passed through meiosis and into progeny. Several independent chromosomes were identified, each with newly created centromeres localized over the repeat arrays where they were directed. The new centromeres were self-sustaining and stably transmitted chromosomes to progeny in the absence of the LexA-CENH3 activator. Our results demonstrate the feasibility of using synthetic centromeres for karyotype engineering applications.

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Synthetic chromosomes, genomes, viruses, and cells

Cited by 80 publications

References 102 publications

Assembly of plant virus agroinfectious clones using biological material or DNA synthesis

Assembly of plant virus agroinfectious clones using biological material or DNA synthesis

Recent Advances in Genomics-Based Approaches for the Development of Intracellular Bacterial Pathogen Vaccines

Synthetic maize centromeres transmit chromosomes across generations

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