Synthetic cystic fibrosis sputum medium diminishes <i>Burkholderia cenocepacia</i> antifungal activity against <i>Aspergillus fumigatus</i> independently of phenylacetic acid production

Lightly, Tasia Joy; Phung, Ryan; Sorensen, John L.; Cardona, Silvia T.

doi:10.1139/cjm-2016-0705

Cited by 14 publications

(8 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Dysfunction of CFTR affects the transepithelial fluid and the ion transport of the exocrine glands and leads to a nutrient rich, thick airway surface liquid in the lungs. This highly viscous mucus cannot be cleared effectively by the muco-ciliary transport system supporting the colonization of the airways by bacterial and fungal pathogens (Lightly et al, 2017). Chronic infections are among the primary causes of mortality of CF patients, since both the infecting microbes and the inflammation response cause irreversible damage in the lung tissue and lead to a respiratory failure (LiPuma, 2010).…”

Section: Introductionmentioning

confidence: 99%

In vitro Interactions of Pseudomonas aeruginosa With Scedosporium Species Frequently Associated With Cystic Fibrosis

et al. 2019

View full text Add to dashboard Cite

Members of the Scedosporium apiospermum species complex are the second most frequently isolated pathogens after Aspergillus fumigatus from cystic fibrosis (CF) patients with fungal pulmonary infections. Even so, the main risk factors for the infection are unrevealed. According to previous studies, bacterial infections might reduce the risk of a fungal infection, but an antibacterial therapy may contribute to the airway colonization by several fungal pathogens. Furthermore, corticosteroids, which are often used to reduce lung inflammation in children and adults with CF, are also proved to enhance the growth of A. fumigatus in vitro. Considering all the above discussed points, we aimed to test how Pseudomonas aeruginosa influences the growth of scedosporia and to investigate the potential effect of commonly applied antibacterial agents and corticosteroids on Scedosporium species. Direct interactions between fungal and bacterial strains were tested using the disk inhibition method. Indirect interactions via volatile compounds were investigated by the plate-in-plate method, while the effect of bacterial media-soluble molecules was tested using a modified cellophane assay and also in liquid culture media conditioned by P. aeruginosa. To test the effect of bacterial signal molecules, antibacterial agents and corticosteroids on the fungal growth, the broth microdilution method was used. We also investigated the germination ability of Scedosporium conidia in the presence of pyocyanin and diffusible signal factor by microscopy. According to our results, P. aeruginosa either inhibited or enhanced the growth of scedosporia depending on the culture conditions and the mode of interactions. When the two pathogens were cultured physically separately from each other in the plate-in-plate tests, the presence of the bacteria was able to stimulate the growth of several fungal isolates. While in direct physical contact, bacterial strains inhibited the fungal growth. This effect might be attributed to bacterial signal molecules, which also proved to inhibit the germination and growth of scedosporia. In addition, antibacterial agents showed growth-promoting, while corticosteroids exhibited growth inhibitory effect on several Scedosporium isolates. These data raise the possibility that a P. aeruginosa infection or a previously administered antibacterial therapy might be able to increase the chance of a Scedosporium colonization in a CF lung.

show abstract

Section: Introductionmentioning

confidence: 99%

In vitro Interactions of Pseudomonas aeruginosa With Scedosporium Species Frequently Associated With Cystic Fibrosis

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Phenylacetic acid (PAA) is a signal molecule that could be involved in microbial interactions with the host 57 . The phenylacetic acid (PAA) catabolic pathway was shown to be required for the full pathogenicity of B. cenocepacia in a Caenorhabditis elegans nematode infection model 58 .…”

Section: Discussionmentioning

confidence: 99%

Environmental interactions are regulated by temperature in Burkholderia seminalis TC3.4.2R3

Gonçalves

Denman

Ferreira

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Burkholderia seminalis strain TC3.4.2R3 is an endophytic bacterium isolated from sugarcane roots that produces antimicrobial compounds, facilitating its ability to act as a biocontrol agent against phytopathogenic bacteria. In this study, we investigated the thermoregulation of B. seminalis TC3.4.2R3 at 28 °C (environmental stimulus) and 37 °C (host-associated stimulus) at the transcriptional and phenotypic levels. The production of biofilms and exopolysaccharides such as capsular polysaccharides and the biocontrol of phytopathogenic fungi were enhanced at 28 °C. At 37 °C, several metabolic pathways were activated, particularly those implicated in energy production, stress responses and the biosynthesis of transporters. Motility, growth and virulence in the Galleria mellonella larvae infection model were more significant at 37 °C. Our data suggest that the regulation of capsule expression could be important in virulence against G. mellonella larvae at 37 °C. In contrast, B. seminalis TC3.4.2R3 failed to cause death in infected BALB/c mice, even at an infective dose of 10 7 CFU.mL −1 . We conclude that temperature drives the regulation of gene expression in B. seminalis during its interactions with the environment.

show abstract

“…The "culturomics" extended-culture approach can culture bacteria previously considered "uncultivable" [48]. It also explores potential microbial interactions identified in meta-genetic studies and characterizes bacterial metabolites of interest [49][50][51]. In CF, extensive culture-enriched airway microbiome profiling identified bacterial families, such as Ruminococcaceae or Bacteriovoracaceae, in CF sputa not detected by 16S rDNA sequencing alone [52].…”

Section: Culture-based Strategymentioning

confidence: 99%

The Microbiome in Cystic Fibrosis Pulmonary Disease

Françoise

Héry-Arnaud

2020

Genes

View full text Add to dashboard Cite

Cystic fibrosis (CF) is a genetic disease with mutational changes leading to profound dysbiosis, both pulmonary and intestinal, from a very young age. This dysbiosis plays an important role in clinical manifestations, particularly in the lungs, affected by chronic infection. The range of microbiological tools has recently been enriched by metagenomics based on next-generation sequencing (NGS). Currently applied essentially in a gene-targeted manner, metagenomics has enabled very exhaustive description of bacterial communities in the CF lung niche and, to a lesser extent, the fungi. Aided by progress in bioinformatics, this now makes it possible to envisage shotgun sequencing and opens the door to other areas of the microbial world, the virome, and the archaeome, for which almost everything remains to be described in cystic fibrosis. Paradoxically, applying NGS in microbiology has seen a rebirth of bacterial culture, but in an extended manner (culturomics), which has proved to be a perfectly complementary approach to NGS. Animal models have also proved indispensable for validating microbiome pathophysiological hypotheses. Description of pathological microbiomes and correlation with clinical status and therapeutics (antibiotic therapy, cystic fibrosis transmembrane conductance regulator (CFTR) modulators) revealed the richness of microbiome data, enabling description of predictive and follow-up biomarkers. Although monogenic, CF is a multifactorial disease, and both genotype and microbiome profiles are crucial interconnected factors in disease progression. Microbiome-genome interactions are thus important to decipher.

show abstract

Synthetic cystic fibrosis sputum medium diminishes Burkholderia cenocepacia antifungal activity against Aspergillus fumigatus independently of phenylacetic acid production

Cited by 14 publications

References 48 publications

In vitro Interactions of Pseudomonas aeruginosa With Scedosporium Species Frequently Associated With Cystic Fibrosis

In vitro Interactions of Pseudomonas aeruginosa With Scedosporium Species Frequently Associated With Cystic Fibrosis

Environmental interactions are regulated by temperature in Burkholderia seminalis TC3.4.2R3

The Microbiome in Cystic Fibrosis Pulmonary Disease

Contact Info

Product

Resources

About