Synthetic design of methanotroph co-cultures and their immobilization within polymers containing magnetic nanoparticles to enhance methanol production from wheat straw-based biogas

Patel, Sanjay K.S.; Gupta, Rahul; Kalia, Vipin Chandra; Lee, Jung-Kul

doi:10.1016/j.biortech.2022.128032

Cited by 28 publications

(3 citation statements)

References 46 publications

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“…The cell dry mass was analyzed using constant-weight measurements at 70 °C [ 19 ]. The production of methanol in batch cultures was examined in 120 mL bottles (Sigma-Aldrich, USA) containing 20 mL of phosphate buffer (100 mM, pH 6.8) and MgCl 2 (as MDH inhibitor), using methanotrophs inoculum at 3.0 mg DCM/mL from simulated biogas (CH 4 , 30% ( v / v ), and CO 2 , 15% ( v / v )) for incubation at 30 °C for 48 h [ 21 ].…”

Section: Methodsmentioning

confidence: 99%

“…The methanol concentration was assessed using an Agilent 7890A chromatography system equipped with a flame ionization detector and an Agilent 19091 J-413 (HP-5) column (Santa Clara, CA, USA) [ 19 ]. Validation of whole-cell encapsulation within CuFe 2 O 4 -containing polymeric matrix was performed via field-emission scanning electron microscopy (FE-SEM, JEOL, Tokyo, Japan) [ 21 ]. X-ray diffraction (XRD) analysis was performed using an X’pert PRO MPD, Malvern PANalytical, Malvern, UK).…”

Section: Methodsmentioning

confidence: 99%

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Encapsulation of Methanotrophs within a Polymeric Matrix Containing Copper- and Iron-Based Nanoparticles to Enhance Methanol Production from a Simulated Biogas

Patel,

Gupta,

Kim

et al. 2023

Polymers

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The production of renewable energy or biochemicals is gaining more attention to minimize the emissions of greenhouse gases such as methane (CH4) and carbon dioxide for sustainable development. In the present study, the influence of copper (Cu)- and iron (Fe)-based nanoparticles (NPs), such as Cu, Fe3O4, and CuFe2O4, was evaluated during the growth of methanotrophs for inoculum preparation and on the development of a polymeric-matrix-based encapsulation system to enhance methanol production from simulated biogas (CH4 and CO2). The use of simulated biogas feed and the presence of NP-derived inoculums produce a remarkable enhancement in methanol production up to 149% and 167% for Methyloferula stellata and Methylocystis bryophila free-cells-based bioconversion, respectively, compared with the use of pure CH4 as a control feed during the growth stage. Furthermore, these methanotrophs encapsulated within a polymeric matrix and NPs-based systems exhibited high methanol production of up to 156%, with a maximum methanol accumulation of 12.8 mmol/L over free cells. Furthermore, after encapsulation, the methanotrophs improved the stability of residual methanol production and retained up to 62.5-fold higher production potential than free cells under repeated batch reusability of 10 cycles. In the presence of CH4 vectors, methanol production by M. bryophila improved up to 16.4 mmol/L and retained 20% higher recycling stability for methanol production in paraffin oil. These findings suggest that Cu and Fe NPs can be beneficially employed with a polymeric matrix to encapsulate methanotrophs and improve methanol production.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Encapsulation of Methanotrophs within a Polymeric Matrix Containing Copper- and Iron-Based Nanoparticles to Enhance Methanol Production from a Simulated Biogas

Patel,

Gupta,

Kim

et al. 2023

Polymers

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“…Methanotrophs are bacteria capable of utilizing methane as their sole carbon and energy source (Patt et al, 1974;Sahoo et al, 2021). These methane-oxidizing bacteria act as a biological methane sink for the potent greenhouse gas methane (Patel et al, 2022). Some of methanotrophs are aerobic, whereas others are anaerobic (Hanson and Hanson, 1996;Strong et al, 2016;Case et al, 2017;Su et al, 2017;Steinsdóttir et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Transcriptional and metabolomic responses of Methylococcus capsulatus Bath to nitrogen source and temperature downshift

Bedekar,

Deewan,

Jagtap

et al. 2023

Front. Microbiol.

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Methanotrophs play a significant role in methane oxidation, because they are the only biological methane sink present in nature. The methane monooxygenase enzyme oxidizes methane or ammonia into methanol or hydroxylamine, respectively. While much is known about central carbon metabolism in methanotrophs, far less is known about nitrogen metabolism. In this study, we investigated how Methylococcus capsulatus Bath, a methane-oxidizing bacterium, responds to nitrogen source and temperature. Batch culture experiments were conducted using nitrate or ammonium as nitrogen sources at both 37°C and 42°C. While growth rates with nitrate and ammonium were comparable at 42°C, a significant growth advantage was observed with ammonium at 37°C. Utilization of nitrate was higher at 42°C than at 37°C, especially in the first 24 h. Use of ammonium remained constant between 42°C and 37°C; however, nitrite buildup and conversion to ammonia were found to be temperature-dependent processes. We performed RNA-seq to understand the underlying molecular mechanisms, and the results revealed complex transcriptional changes in response to varying conditions. Different gene expression patterns connected to respiration, nitrate and ammonia metabolism, methane oxidation, and amino acid biosynthesis were identified using gene ontology analysis. Notably, key pathways with variable expression profiles included oxidative phosphorylation and methane and methanol oxidation. Additionally, there were transcription levels that varied for genes related to nitrogen metabolism, particularly for ammonia oxidation, nitrate reduction, and transporters. Quantitative PCR was used to validate these transcriptional changes. Analyses of intracellular metabolites revealed changes in fatty acids, amino acids, central carbon intermediates, and nitrogen bases in response to various nitrogen sources and temperatures. Overall, our results offer improved understanding of the intricate interactions between nitrogen availability, temperature, and gene expression in M. capsulatus Bath. This study enhances our understanding of microbial adaptation strategies, offering potential applications in biotechnological and environmental contexts.

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Methane fermentation to methanol (biological gas‐to‐liquid process) usingMethylotuvimicrobium buryatense5GB1C

Priyadarsini,

Khaire,

Barbora

et al. 2023

J Adv Manuf & Process

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Methanol is a potential alternate liquid transportation fuel for blending with gasoline. Biochemical conversion of methane to methanol is a green process for methanol production. This paper reports biochemical methanol production using type I γ‐proteobacteria Methylotuvimicrobium buryatense, which has particular importance from the viewpoint of scalable biological gas to liquid processes for industrial application. A statistical design of experiments (at the serum bottle level) was used to optimize fermentation parameters. Enhancement in methanol accumulation was attempted using methanol dehydrogenase inhibitors. This was followed by a validation experiment run in a bioreactor at optimum conditions. At optimum conditions (pH = 7, phosphate concentration = 140 mM, temperature = 25°C) and optical density (600 nm) of 0.3, a methanol titer of 8.54 mM was achieved in 24 h (methane conversion = 20.8%). The addition of a methanol dehydrogenase inhibitor (0.5 mM Ethylenediaminetetraacetic acid) enhanced the methanol concentration to 10.37 mM. Experiments in a 3.7 L bioreactor using 1.68 bar headspace pressure and optical density (600 nm) of 0.1 yielded 23.7 mM methanol in 24 h (methane conversion = 47.8%). The methanol titers obtained using M. buryatense 5GB1C in 24 h fermentation are significantly higher than several previously reported methanotrophs. These results demonstrate the potential of M. buryatense 5GB1C for the biochemical synthesis of methanol.

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Synthetic design of methanotroph co-cultures and their immobilization within polymers containing magnetic nanoparticles to enhance methanol production from wheat straw-based biogas

Cited by 28 publications

References 46 publications

Encapsulation of Methanotrophs within a Polymeric Matrix Containing Copper- and Iron-Based Nanoparticles to Enhance Methanol Production from a Simulated Biogas

Encapsulation of Methanotrophs within a Polymeric Matrix Containing Copper- and Iron-Based Nanoparticles to Enhance Methanol Production from a Simulated Biogas

Transcriptional and metabolomic responses of Methylococcus capsulatus Bath to nitrogen source and temperature downshift

Methane fermentation to methanol (biological gas‐to‐liquid process) usingMethylotuvimicrobium buryatense5GB1C

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