Synthetic heteroprostanoids of A- and E-types as novel non-comprehensive inhibitors of adenylyl cyclase in rat hepatocytes

Hepatoprotective action of prostaglandin A2 analogs under CCl4‐induced liver injury in vitro

“…Hepatocytes were isolated from male Wistar rats (250–300 g) by a non‐recirculating, single‐stage, non‐enzymatic perfusion of the livers through the portal vein, following a procedure described by Berry and Friend, 12 with modifications 13 . Briefly, the livers were perfused with Ringer–Lock solution (120 mM NaCl, 5 mM KCl, 24 mM NaHCO 3 , 27 mM sodium citrate, 1% D ‐glucose, pH 7.4) at room temperature at a flow of 7.5–10.0 mL/min for 10 min.…”

4‐Isoxazolines: Scaffolds for Organic Synthesis

“…[1,[2][3][4][5][6][7][8] With monosubstituted alkynes such as alkyl-or phenylacetylene, however, 5-substituted 4-isoxazolines are obtained regiospecifically. On the other hand, electron-deficient acetylenes (e.g., methyl propiolate or cyanoacetylene) give mixture of regioisomers or lead to the selective synthesis of 4-substi-tuted 4-isoxazolines.…”

“…[3][4][5][6][7] These heterocyclic compounds have attracted considerable interest due to their readiness to undergo rearrangement reactions, making them of interest from both synthetic and mechanistic points of view. The reactivity of this ring system is mainly due to the relatively low thermochemical stability of the N-O bond associated with the π system.…”

Synthesis of spirocyclic Δ4-isoxazolines via [3 + 2] cycloaddition of indanone-derived ketonitrones with alkynes

“…Isoxazolines are part of an important class of N,O-containing heterocycles, since they are well known for biological properties 1 and could be used as versatile intermediates for the synthesis of many complex compounds. 2 Among the many methods, the cycloaddition of nitrones has been widely used in the synthesis of this skeleton.…”