Synthetic Human Calcitonin: Analysis of Antibodies Obtained From Various Animal Species and Determination of Immunoreactive Hormone in Human Sera

Dietrich, Felix M.; Hunziker, W.; Fischer, Jan A.

doi:10.1530/acta.0.0800465

Cited by 59 publications

(18 citation statements)

References 26 publications

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“…Blood samples were drawn before and 120 min after the onset of infusions. Plasma Ca 2+ was measured by atomic absorption; blood gincose enzymatically; serum insulin, plasma C-peptide, plasma glucagon [7,8], and plasma calcitonin [9] were measured by immunoassay. Plasma glucagon was assayed using the antibody 30 K (pool 4, lot 3,1978), kindly provided by Dr. R. Linger, Dallas, Texas.…”

Section: Methodsmentioning

confidence: 99%

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Effects of calcium and calcitonin on circulating levels of glucagon and glucose in diabetes mellitus

et al. 1981

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Summary. The effects of Ca 2+ and calcitonin infusions on circulating glucagon, glucose, C-peptide, Ca 2+, and calcitonin were investigated in hyperglucagonaemic insulin-dependent diabetics. In 14 insulin-deprived diabetics and 12 healthy volunteers 2 h infusions of saline (0.154 tool/l), Ca 2+ (0.375 mmol/kg body weight), and ealcitonin (4.5 IU/kg body weight) were performed. There were no significant changes during saline infusion. In the diabetics, Ca 2+ infusions induced a rise of plasma Ca 2+ up to 3.2 _ 0.1 mmol/1 and a fall of circulating glucagon (--26.4 +_ 5.7%; p < 0.001) and glucose (-23.3 _+ 3.6%; p < 0.05). Plasma calcitonin rose to twice basal values (p < 0.025). During calcitonin infusions plasma Ca 2+ decreased slightly to 2.1 + 0.2 mmol/1; a fall was found in both glucose (-24.4 _+ 4.0%; p < 0.05) and circulating glucagon (-22.5 +_ 4.3%; p < 0.001), Two groups of 6 healthy volunteers were subjected to saline and Ca 2+, or to Ca 2+ and calcitonin infusions. Both Ca 2+ and calcitonin infusions induced a fall of serum insulin (-30.1 + 6.6%; p < 0.05). Calcitonin depressed circulating glucagon by -18.6 +_ 4.4% (p < 0.025), whereas during Ca 2+ infusions glucagon decreased only by -6,5 _+ 1.9% (p > 0.1). We conclude from our results that an increase of circulating calcitonin induced by Ca 2+ infusions or by exogenous calcitonin administration appears to depress elevated circulating glucagon and glucose in insulin-dependent diabetics.

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Section: Methodsmentioning

confidence: 99%

“…Calcitonin was assayed using a commercially available kit system (RIA-mat calcitonin, Byk-Mallinckrodt, West Germany). The antiserum in this system was raised in a goat against synthetic human calcitonin according to Dietrich et al [9]. The doubleantibody separation technique was used in tbe presence of polyethylene glycol.…”

Section: Methodsmentioning

confidence: 99%

Effects of calcium and calcitonin on circulating levels of glucagon and glucose in diabetes mellitus

et al. 1981

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“…(9), adrenocorticotropin (ACTH)- , somatostatin, and purified porcine insulin were donated by W. Rittel (10) were iodinated with chloramine-T as described (11) on Bio-Gel P-6. The radioactivity eluted from the cells with 0.1 M acetic acid consisted predominantly of fragments of salmon calcitonin both in the absence and presence of ACTH; binding of fragments of radioactive salmon calcitonin to 8866 cells varied between 30 and 70%, but the binding could not be inhibited by 50 nM unlabeled salmon calcitonin (not shown).…”

Section: Methodsmentioning

confidence: 99%

Calcitonin and calcium ionophores: cyclic AMP responses in cells of a human lymphoid line.

Moran¹,

Hunziker²,

Fischer³

1978

Proc. Natl. Acad. Sci. U.S.A.

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Receptors for calcitonin, as assayed by the specific binding of 'SI-labeled salmon calcitonin and stimulation of cyclic AMP formation, were found in 886 cels derived from a human lymphoid line. The affinity of calcitonin from different species and of various analogues of human calcitonin for the binding sites and their ability to stimulate cyclic AMP formation were closely related to their hypocalcemic activity and presumably reflected biological properties of the hormones. Cell Culture, Isolation, and Incubation. The 8866 cells were derived from a patient with chronic myelogeneous leukemia (6) and were generously provided by S. J. Marx (NIAMDD). They were cultured in RPMI 1640 medium with 2 mM L-glutamine, supplemented with 10% fetal calf serum (vol/vol; Gibco, Glasgow, Scotland) and 0.5 ,ug of neomycin sulfate per ml, under a humid atmosphere of 95% air/5% CO2 at 370. Cells were fed two to three times weekly by 1:3 dilution in fresh growth medium. The cells were harvested at the late phase of exponential growth by sedimentation for 10 min at 300 X g at 40, washed three times in the incubation medium, and resuspended to a density of 107 cells per ml.The incubation medium contained 25 mM The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact.

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“…The methanol was evaporated, and the samples were lyophilized after the addition of 10 ml of 0.1 M acetic acid. The precipitates were dissolved in 1 ml of0.1 M acetic acid, and CT was analyzed on a reversed-phase HPLC system (Altex, Berkeley, CA) with Nucleosil C-18 (Machery-Nagel GmbH, Dfiren, (20). The antibodies (goat 6A obtained on day 143) used were predominantly directed to determinants located in the COOH-terminal parts ofthe CT-(1-32) molecule.…”

mentioning

confidence: 99%

Calcitonin: regional distribution of the hormone and its binding sites in the human brain and pituitary.

Fischer¹,

Tobler²,

Kaufmann³

et al. 1981

Proc. Natl. Acad. Sci. U.S.A.

174

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Immunoreactive calcitonin (CT), indistinguishable from human CT-(1-32) and its sulfoxide, has been identified in extracts of the hypothalamus, the pituitary, and the thyroid obtained from human subjects at autopsy. CT concentrations were highest in a region encompassing the posterior hypothalamus, the median eminence, and the pituitary; intermediate in the substantia nigra, the anterior hypothalamus, the globus pallidus, and the inferior colliculus; and low in the caudate nucleus, the hippocampus, the amygdala, and the cerebral and cerebellar cortices. Specific CT binding measured with "MI-labeled salmon CT was high-

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Synthetic Human Calcitonin: Analysis of Antibodies Obtained From Various Animal Species and Determination of Immunoreactive Hormone in Human Sera

Cited by 59 publications

References 26 publications

Effects of calcium and calcitonin on circulating levels of glucagon and glucose in diabetes mellitus

Effects of calcium and calcitonin on circulating levels of glucagon and glucose in diabetes mellitus

Calcitonin and calcium ionophores: cyclic AMP responses in cells of a human lymphoid line.

Calcitonin: regional distribution of the hormone and its binding sites in the human brain and pituitary.

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