Synthetic hydrogels for controlled stem cell differentiation

Liu, Shao Qiong; Tay, Pei Kun Richie Richie; Khan, Majad; Ee, Pui Lai Rachel; Hedrick, James L.; Yang, Yi Yan

doi:10.1039/b916705f

Cited by 129 publications

(98 citation statements)

References 192 publications

(291 reference statements)

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“…16 Further, because of the hydrophilic nature of hydrogels, ECM proteins such as fibronectin (FN), laminin (LM) and vitronectin typically do not absorb to the gel surface. 17 Therefore, various cell-interacting components, such as ECM molecules, small peptide, and glycoproteins need to be incorporated to these culture systems. 18,19 Combining with appropriate mechanical properties, chemical composition and biological signals, synthetic hydrogels have been modified and designed in a variety of ways to create suitable microenvironment for controlling ES cells differentiation toward preferential specific lineage.…”

Section: Introductionmentioning

confidence: 99%

Dynamic Behavior and Spontaneous Differentiation of Mouse Embryoid Bodies on Hydrogel Substrates of Different Surface Charge and Chemical Structures

Liu

Chen

Yang

et al. 2011

Tissue Engineering Part A

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Differentiation of embryoid bodies (EBs) into particular cell lineages has been extensively studied. There is an increasing interest in the effect of soft hydrogel scaffolds on the behavior of EBs, such as the initial adhesion, dynamic morphology change, and differentiation. In this study, without adding any other bioactive factors in the serum-containing medium, dynamic behaviors of mouse EBs loaded on the surface of hydrogels with different surface charge and chemical structures are investigated. EBs adhered quickly to negatively charged poly(sodium p-styrene sulfonate) (PNaSS) hydrogels, which facilitates EBs spreading, migration, and differentiation into three germ layers with high efficiency of cardiomyocytes differentiation, similar to that on gelatin coated polystyrene (PS) culture plate. While on neutral poly(acrylamide) (PAAm) hydrogels, EBs maintained the initial spherical morphology with high expression of pluripotency-related markers in the short culture periods, and then showed the significantly greater levels of selected endoderm markers after long-time culture. EBs cultured on negatively charged poly(2-acrylamido-2-methyl-propane sulfonic acid sodium salt) (PNaAMPS) gels demonstrated the analogous behaviors with that of neutral PAAm gels at early differentiation phase (day 4 + 1). Then, their adhesion, spreading and differentiation were quite similar to that on negatively charged PNaSS gels. The correlation between surface properties of hydrogels and EBs differentiation was discussed.

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Section: Introductionmentioning

confidence: 99%

Dynamic Behavior and Spontaneous Differentiation of Mouse Embryoid Bodies on Hydrogel Substrates of Different Surface Charge and Chemical Structures

Liu

Chen

Yang

et al. 2011

Tissue Engineering Part A

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“…PEG-based hydrogel is synthesized commonly via (1) the radical polymerization, e.g., redox or photo-initiated polymerization of vinyl groups on PEG macromers; (2) the Michael addition chemistry where PEG macromers with thiol groups react PEG macromers with α,-unsaturated carbonyl groups; (3) the click chemistry where PEG macromers with azide groups react PEG macromers with alkyne groups in the presence of Cu 2+ as a catalyst; and (4) the enzyme-catalyzed reaction [76]. Because the radical polymerization is fast but the step-growth polymerization, e.g., the Michael addition chemistry, can fine-tune the structure of the gel's network, a mix-mode polymerization approach, such as the thiol-acrylate photopolymerization, has been developed to take advantages of the chain-growth and step-growth mechanisms [77].…”

Section: Synthetic Hydrogelmentioning

confidence: 99%

Microfluidic-Based Synthesis of Hydrogel Particles for Cell Microencapsulation and Cell-Based Drug Delivery

2012

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Encapsulation of cells in hydrogel particles has been demonstrated as an effective approach to deliver therapeutic agents. The properties of hydrogel particles, such as the chemical composition, size, porosity, and number of cells per particle, affect cellular functions and consequently play important roles for the cell-based drug delivery. Microfluidics has shown unparalleled advantages for the synthesis of polymer particles and been utilized to produce hydrogel particles with a well-defined size, shape and morphology. Most importantly, during the encapsulation process, microfluidics can control the number of cells per particle and the overall encapsulation efficiency. Therefore, microfluidics is becoming the powerful approach for cell microencapsulation and construction of cell-based drug delivery systems. In this article, I summarize and discuss microfluidic approaches that have been developed recently for the synthesis of hydrogel particles and encapsulation of cells. I will start by classifying different types of hydrogel material, including natural biopolymers and synthetic polymers that are used for cell encapsulation, and then focus on the current status and challenges of microfluidic-based approaches. Finally, applications of cell-containing hydrogel particles for cell-based drug delivery, particularly for cancer therapy, are discussed.

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“…[16,17] Additionally, PEG-coated medical devices prevent non-specific protein adhesion which reduces immune and inflammatory responses, while PEG macromer chemistry versatility facilitates the incorporation of chemical and physical cues for stem cell adhesion and controlled differentiation. [18] The marriage of advanced PEG synthetic chemistry with the current stem cell knowledge has led to a matrix that mimics the stem cell microenvironment. [18,19] The PET surface modification process ( Figure 1) in this work consists of two components: a poly(vinylamine) (PVA) backbone that acts a crosslinker, and a PEG outer layer.…”

Section: Introductionmentioning

confidence: 99%

“…[18] The marriage of advanced PEG synthetic chemistry with the current stem cell knowledge has led to a matrix that mimics the stem cell microenvironment. [18,19] The PET surface modification process ( Figure 1) in this work consists of two components: a poly(vinylamine) (PVA) backbone that acts a crosslinker, and a PEG outer layer. The first step involves the reaction of PVA with PET where PVA nitrogens nucleophilically attack the PET ester groups forming amide linkages.…”

Section: Introductionmentioning

confidence: 99%

Low Thrombogenicity Coating of Nonwoven PET Fiber Structures for Vascular Grafts

Dimitrievska

Maire

Diaz-Quijada

et al. 2011

Macromolecular Bioscience

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Vascular PET grafts (Dacron) have shown good performance in large vessels (≥6 mm) applications. To address the urgent unmet need for small‐diameter (2–6 mm) vascular grafts, proprietary high‐compliance nonwoven PET fiber structures were modified with various PEG concentrations using PVA as a cross‐linking agent, to fabricate non‐thrombogenic mechanically compliant vascular grafts. The blood compatibility assays measured through platelet adhesion (SEM and mepacrine dye) and platelet activation (morphological changes, P‐selectin secretion, and TXB2 production) demonstrate that functionalization using a 10% PEG solution was sufficient to significantly reduce platelet adhesion/activation close to optimal literature‐reported levels observed on carbon‐coated ePTFE.

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Synthetic hydrogels for controlled stem cell differentiation

Cited by 129 publications

References 192 publications

Dynamic Behavior and Spontaneous Differentiation of Mouse Embryoid Bodies on Hydrogel Substrates of Different Surface Charge and Chemical Structures

Dynamic Behavior and Spontaneous Differentiation of Mouse Embryoid Bodies on Hydrogel Substrates of Different Surface Charge and Chemical Structures

Microfluidic-Based Synthesis of Hydrogel Particles for Cell Microencapsulation and Cell-Based Drug Delivery

Low Thrombogenicity Coating of Nonwoven PET Fiber Structures for Vascular Grafts

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