Synthetic microbiology in sustainability applications

Jones, Ethan M.; Marken, John P.; Silver, Pamela A.

doi:10.1038/s41579-023-01007-9

Cited by 12 publications

(1 citation statement)

References 184 publications

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“…With the development of synthetic biology technology, microbial cell factories have been applied to produce heme-related products. − Impossible Foods Inc. inserted the key genes of the heme synthesis pathway into the Pichia pastoris genome, and they further integrated the soybean hemoglobin gene to realize industrial production of hemoglobin . Zhao et al improved the heme synthesis in Escherichia coli by optimizing the metabolic flux of the heme synthesis pathway .…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Green Production of Heme by Deleting Odor-Related Genes from Bacillus amyloliquefaciens Based on CRISPR/Cas9n

Jiang,

Zou,

Jiang

et al. 2024

J. Agric. Food Chem.

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Heme is a crucial component in endowing plant-based meat analogs with flavor and color. This study aimed to develop a green strategy for heme production by reducing fermentation off-odor and accelerating heme synthesis. First, an efficient CRISPR/Cas9n system was constructed in Bacillus amyloliquefaciens to construct the odor-reducing chassis cell HZC9nΔGPSU, and the odor substances including the branched-chain short fatty acids, putrescine, and ammonia were reduced by 62, 70, and 88%, respectively. Meanwhile, the hemA gene was confirmed to be the key gene for enhanced heme synthesis. Various hemA genes were compared to obtain the best gene dhemA, and the catalysis mechanism was explained by molecular docking simulation. After further expression of dhemA in HZC9nΔGPSU, the heme titer of HZC9nΔGPSU/pHY-dhemA reached 11.31 ± 0.51 mg/L, 1.70-fold higher than that of HZC9n/pHY-dhemA. The knockout of off-odor-related genes reduced the odor substances and enhanced the heme synthesis, which is promising for the green production of high-quality heme.

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Section: Introductionmentioning

confidence: 99%

Enhancement of Green Production of Heme by Deleting Odor-Related Genes from Bacillus amyloliquefaciens Based on CRISPR/Cas9n

Jiang,

Zou,

Jiang

et al. 2024

J. Agric. Food Chem.

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Rice Growth in the Shadow of Global Warming: Microbes Shed Light on Alleviating Its Heat Stress

Zeng,

Wang,

et al. 2024

J of Sust Agri & Env

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The increasing severity of global climate change has led to more frequent extreme high‐temperature events, significantly damaging rice yield and quality, thus posing a threat to global food security. Research indicates that plant‐microbe interactions can enhance plant growth and overall health under adverse conditions. Therefore, this review aims to explore strategies to improve rice heat tolerance through thermophilic microorganism mediation. This paper systematically summarises the effects of heat stress on both the aboveground and underground parts of rice during its growth stages, identifies the molecular mechanisms by which rice responds to heat stress, and explores the potential roles of microorganisms. Additionally, we review existing studies on microorganisms that alleviate plant heat stress and their mechanisms of action. Through case studies, we explore how microorganisms enhance rice survival in high‐temperature environments by regulating its growth and development, along with their potential applications in sustainable agriculture. In the future, environmentally friendly and efficient microbial inoculants and biofertilizers are expected to be developed based on microbe‐mediated plant heat tolerance mechanisms, which will help mitigate the heat stress challenges crops face under global climate change.

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Quorum sensing regulating the productivity and stability of cross-feeding cocultivation

Chen,

Qu,

Yang

et al. 2024

Chemical Engineering Journal

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Synthetic microbiology in sustainability applications

Cited by 12 publications

References 184 publications

Enhancement of Green Production of Heme by Deleting Odor-Related Genes from Bacillus amyloliquefaciens Based on CRISPR/Cas9n

Enhancement of Green Production of Heme by Deleting Odor-Related Genes from Bacillus amyloliquefaciens Based on CRISPR/Cas9n

Rice Growth in the Shadow of Global Warming: Microbes Shed Light on Alleviating Its Heat Stress

Quorum sensing regulating the productivity and stability of cross-feeding cocultivation

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