Synthetic multivalent DNAzymes for enhanced hydrogen peroxide catalysis and sensitive colorimetric glucose detection

Yang, Deng-Kai; Kuo, Chun-Chao; Chen, Lin-Chi

doi:10.1016/j.aca.2014.11.031

Cited by 29 publications

(14 citation statements)

References 36 publications

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“…It is known that the identity of the base composition and length of the linker sequence can influence the activities of monovalent and multivalent DNAzymes [17]. To remove the potential for linker-dependent effects on our analyses, we chose to use the relatively simple TT linker sequence in order to obtain a relatively unconfounded effect of multimerisation on DNAzyme activity [20,21]. .…”

Section: Dnazymementioning

confidence: 99%

“…One hypothesis that explains the rate-enhancing effect of mulitimerisation is that multimeric DNAzyme structures potentially create additional high-affinity binding sites for haem incorporation, thereby resulting in more than one active site per DNAzyme monomer. However, in certain cases, there is no direct correlation between the number of units and the activity of the multimer [20,21]. This suggests that certain structural features do not necessarily support activity enhancement in multimers.…”

Section: Introductionmentioning

confidence: 99%

“…Multimerisation of discrete units of G-quadruplexes to form multivalent DNAzymes is an emerging design strategy aimed at enhancing the peroxidase activities of DNAzymes [20,21]. In these multiple active site structures, individual DNAzyme molecular units are joined together by polynucleotide linkers that do not participate directly in the reactions.…”

Section: Introductionmentioning

confidence: 99%

“…It is thought that this rate enhancement results from synergistic interactions between the individual subunits. In these arrangements, a multivalent DNAzyme sequence would be more active than the sum activities of an equal number of monomeric sequence units [20,21]. One hypothesis that explains the rate-enhancing effect of mulitimerisation is that multimeric DNAzyme structures potentially create additional high-affinity binding sites for haem incorporation, thereby resulting in more than one active site per DNAzyme monomer.…”

Section: Introductionmentioning

confidence: 99%

“…While multimerisation has the potential to generate more active DNAzymes, only a handful of DNAzyme multimers have been reported [20][21][22]. It is not entirely clear what factors determine the catalytic properties of multimers when compared to their monomeric G-quadruplexes.…”

Section: Introductionmentioning

confidence: 99%

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Catalytic Activities of Multimeric G-Quadruplex DNAzymes

et al. 2019

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G-quadruplex DNAzymes are short DNA aptamers with repeating G4 quartets bound in a non-covalent complex with hemin. These G4/Hemin structures exhibit versatile peroxidase-like catalytic activity with a wide range of potential applications in biosensing and biotechnology. Current efforts are aimed at gaining a better understanding of the molecular mechanism of DNAzyme catalysis as well as devising strategies for improving their catalytic efficiency. Multimerisation of discrete units of G-quadruplexes to form multivalent DNAzyes is an emerging design strategy aimed at enhancing the peroxidase activities of DNAzymes. While this approach holds promise of generating more active multivalent G-quadruplex DNAzymes, few examples have been studied and it is not clear what factors determine the enhancement of catalytic activities of multimeric DNAzymes. In this study, we report the design and characterisation of multimers of five G-quadruplex sequences (AS1411, Bcl-2, c-MYC, PS5.M and PS2.M). Our results show that multimerisation of G-quadruplexes that form parallel structure (AS1411, Bcl-2, c-MYC) leads to significant rate enhancements characteristic of cooperative and/or synergistic interactions between the monomeric units. In contrast, multimerisation of DNA sequences that form non-parallel structures (PS5.M and PS2.M) did not exhibit similar levels of synergistic increase in activities. These results show that design of multivalent G4/Hemin structures could lead to a new set of versatile and efficient DNAzymes with enhanced capacity to catalyse peroxidase-mimic reactions.

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Section: Dnazymementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Catalytic Activities of Multimeric G-Quadruplex DNAzymes

et al. 2019

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State of the Art and Emerging Applications

Chen

Weng

Lee

2018

Aptamers for Analytical Applications

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Smart DNA Network Capturing and Destructing Tumor‐Derived Small Extracellular Vesicles at Tumor Sites for Localized Cancer Therapy

Yang,

Tang,

Cheng

et al. 2024

Adv Funct Materials

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Tumor‐derived small extracellular vesicles (sEVs) are proven to play important roles in accelerating the progression of tumors. Destructing the sEVs at tumor sites is therefore a promising route to inhibit tumor progression. Herein, a smart DNA network‐based sEV trap (DNET) is reported, which achieved the specific capture of tumor‐derived sEVs through recognizing sEVs by polyaptamers, and the efficient destruction of sEVs via a photodynamic process at tumor sites. The DNET is constructed through the assembly of two DNA chains generated via rolling circle amplification. The DNA chains contain polyaptamers for capturing sEVs, polyvalent G‐quadruplexes for loading photodynamic reagents, and complementary segments for forming a cross‐linked network. Upon the irradiation of the laser, the captured sEVs in DNET are destructed, causing significant inhibition effects on the migration, invasion, and proliferation of glioblastoma cells. In particular, DNET achieved tumor inhibition rates of 39.70% in the zebrafish tumor model, and 73.10% in the nude mouse tumor model, respectively, demonstrating the significant efficacy of DNET in inhibiting tumor progression.

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Synthetic multivalent DNAzymes for enhanced hydrogen peroxide catalysis and sensitive colorimetric glucose detection

Cited by 29 publications

References 36 publications

Catalytic Activities of Multimeric G-Quadruplex DNAzymes

Catalytic Activities of Multimeric G-Quadruplex DNAzymes

State of the Art and Emerging Applications

Smart DNA Network Capturing and Destructing Tumor‐Derived Small Extracellular Vesicles at Tumor Sites for Localized Cancer Therapy

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