Synthetic Organic Products

Petrowitz, Hans-Joachim

doi:10.1007/978-3-642-88488-7_23

Thin-Layer Chromatography 1969

DOI: 10.1007/978-3-642-88488-7_23

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Synthetic Organic Products

Hans-Joachim Petrowitz¹

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1974

1992

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Cited by 2 publications

References 66 publications

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Comparison of carbon metabolism between chloramphenicol-treated and untreated cultures of Clostridium acetobutylicum

Zhou

Traxler

1992

Appl Microbiol Biotechnol

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Carbon distribution from substrates to products in Clostridium acetobutylicum ATCC 824 was investigated by adding 14C-labeled substrates as tracers. Comparison of carbon conversion between chloramphenicol (CAP)-treated and untreated cultures was also studied. The percentage of 14C recovery in butanol, acetone and ethanol from uniformly labeled [14C]glucose was increased by 17, 25 and 30%, respectively, after CAP addition. The incorporation of 14C in solvents from 14C-labeled acetate and butyrate was also increased by the antibiotic treatment. A total ~4C recovery of 12°70 in all the products from added [14C]Na2CO3 indicates significant heterotrophic CO2 fixation in this microorganism. The ratio of carbon in butanol derived from glucose, acetate and butyrate was about 71:6: 18, and this ratio was unchanged by CAP treatment.

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Comparison of carbon metabolism between chloramphenicol-treated and untreated cultures of Clostridium acetobutylicum

Zhou

Traxler

1992

Appl Microbiol Biotechnol

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Purification and Properties of Dextransucrase and Invertase from Streptococcus mutans

1974

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Invertase (3-D-fructofuranoside fructohydrolase, EC 3.2.1.26) and dextransucrase (a-1, 6-glucan: D-fructose 2-glucosyltransferase, EC 2.4.1.5) were purified from the culture fluids of Streptococcus mutans by chromatography on Sepharose 6B and diethylaminoethyl-cellulose followed by treatment with hydroxyapatite. Each of the enzyme preparations gave a single band when analyzed by either polyacrylamide gel electrophoresis or immunodiffusion. The antigenic determinant of invertase was different from that of dextransucrase on immunodiffusion. The pH optima were 5.25 for invertase and 5.75 for dextransucrase, and the Km

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Synthetic Organic Products

Cited by 2 publications

References 66 publications

Comparison of carbon metabolism between chloramphenicol-treated and untreated cultures of Clostridium acetobutylicum

Comparison of carbon metabolism between chloramphenicol-treated and untreated cultures of Clostridium acetobutylicum

Purification and Properties of Dextransucrase and Invertase from Streptococcus mutans

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