Synthetic peptide SVVYGLR upregulates cell motility and facilitates oral mucosal wound healing

Tanaka, Susumu; Yasuda, Takuji; Hamada, Y.; Kawaguchi, Naomasa; Fujishita, Yohei; Mori, Satoshi; Yokoyama, Yuhki; Yamamoto, Hirofumi; Kogo, Mikihiko

doi:10.1016/j.peptides.2020.170405

Cited by 12 publications

(7 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, our previous study has demonstrated that the local administration of the SV peptide can facilitate the regeneration of the skeletal muscle with scarless healing in a volumetric muscle loss model 17) . The SV peptide could rapidly be degenerated due to its associated short-length peptide, which might lose its potency to activate the TGF-β receptor and contribute to less scar formation as discussed for the effects of the SV peptide in association with the healing of mucosal wounds 16) .…”

Section: Discussionmentioning

confidence: 99%

“…1A and B). Previous studies demonstrate the possible involvement of the activation and subsequent phosphorylation of the TGF-β1 receptor, including the Smad2 and the Smad3, in the SV peptide-induced migration of cardiac and mucosal fibroblasts 15,16) . Therefore, the effect of the TGF-β1 and its inhibitor on the migration activity of HSkMSC and HSMM were investigated.…”

Section: The Involvement Of the Tgf-β1/smad Signaling Pathway In The mentioning

confidence: 99%

“…A total of 2.0×10 4 cells of HSkMSC or HSMM seeded in the upper chamber were allowed to migrate through a fibronectin (10 µg/ mL)-coated polycarbonate membrane with a pore size of 8 µm (Chemotaxicell; Kurabou, Osaka, Japan), as well as serum-free medium was placed in the lower chamber along with SV (20 ng/mL), nonfunctional SV (GYRVLSV; rSV, 20 ng/mL), or PBS as a chemoattractant. Herein, rSV was used as a control, which had the same molecular weight but different sequence from SV and had been proved to be inactive 9,[11][12][13][14][15][16]18) . With respect to the applied dosage of SV or rSV, a previous study reveals that the effect of SV on endothelial cell adhesion is not dose dependent and the minimum effective concentration is 20 ng/mL 10) , which is also effectively employed to examine the effect of SV on other cell lines, including the myogenic cells used in this study [11][12][13][15][16][17][18] .…”

Section: Cell Migration Assaymentioning

confidence: 99%

“…Besides, the N-terminal fragment of the osteopontin reveals the SVVYGLR motif at the C-terminus near the RGD domain during the event of thrombin cleavage. This synthetic peptide (SV peptide) that is composed of seven amino acids has angiogenic potential and promotes the secretion of the type III collagen as well as the differentiation of myocardial-derived fibroblasts into myofibroblasts [9][10][11][12][13][14][15][16] , in which the involvement of the activation of the TGF-β receptor is revealed to be critically important 12) . This short peptide also promotes skeletal muscle regeneration with scarless tissue healing after injury, increasing EMG activities 17) .…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The synthetic peptide SVVYGLR promotes myogenic cell motility via the TGFβ1/Smad signaling pathway and facilitates skeletal myogenic differentiation in vitro

et al. 2021

Self Cite

View full text Add to dashboard Cite

In the present study, we investigated the possible involvement of the TGF-β/Smad signaling pathway in the osteopontin-derived SVVYGLR (SV) peptide-mediated migratory activities of myogenic cells and evaluated the facilitative effects of the SV peptide on the differentiation of myogenic cells in vitro. The SV peptide-induced migration in both human-derived satellite cells and myoblasts was substantially suppressed by the TGF-β1 receptor inhibitor SB431542 or SB505124. Besides, the expression level of the Smad3 phosphorylation was further enhanced by the addition of the SV peptide in comparison with control groups. Furthermore, an increase in the expression of myogenin-positive nuclei and a higher number of nascent myotubes with myosin heavy chain expression was confirmed in cultured myoblasts supplemented with the SV peptide. These results suggest that the involvement of the TGF-β/Smad signaling pathway in the SV peptide-mediated migration and the facilitative effect of the SV peptide on the differentiation of myogenic cells into myotubes.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: The Involvement Of the Tgf-β1/smad Signaling Pathway In The mentioning

confidence: 99%

Section: Cell Migration Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The synthetic peptide SVVYGLR promotes myogenic cell motility via the TGFβ1/Smad signaling pathway and facilitates skeletal myogenic differentiation in vitro

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…SV peptide induces cell migration of oral fibroblast and keratinocytes by activation of TGF-β/Smad signaling pathway in vitro. In vivo, the SV peptide accelerates the healing process of oral mucosal wounds by facilitating angiogenesis and increasing the production of fibroblasts and myofibroblasts in a rat-oral wound model (Tanaka et al, 2020).…”

Section: Synthetic Peptide Svmentioning

confidence: 99%

Bioactive Synthetic Peptides for Oral Tissues Regeneration

et al. 2021

View full text Add to dashboard Cite

Regenerative therapy in oral tissues has gained relevance since tissue loss due to congenital or acquired diseases as well as trauma is a major health problem worldwide. Regeneration depends on the natural capacity of the body and the use of biomaterials and bioactive molecules that can module the processes to replace lost or damaged tissues and restore function. The combined use of scaffolds, cells, and bioactive molecules such as peptides is considered the best approach to achieve tissue regeneration. These peptides can induce diverse cellular processes as they can influence cell behavior and also can modify scaffold properties, giving as a result the enhancement of cell adhesion, proliferation, migration, differentiation, and biomineralization that are required given the complex nature of oral tissues. Specifically, synthetic peptides (SP) have a positive influence on scaffold biocompatibility since in many cases they can mimic the function of a natural peptide or a full-length protein. Besides, they are bioactive molecules easy to produce, process, and modify, and they can be prepared under well-defined and controlled conditions. This review aims to compile the most relevant information regarding advances in SP for dental and periodontal tissue regeneration, their biological effects, and their clinical implications. Even though most of the SP are still under investigation, some of them have been studied in vitro and in vivo with promising results that may lead to preclinical studies. Besides there are SP that have shown their efficacy in clinical trials such as P11-4 for enamel regeneration or caries prevention and ABM/P-15 for cementum, periodontal ligament (PDL), and alveolar bone on a previously calculus- and biofilm-contaminated zone. Also, some SP are commercially available such as PTH1-34 and PepGen P-15 which are used for bone defects treatment.

show abstract

Osteopontin-derived synthetic peptide SVVYGLR upregulates functional regeneration of oral and maxillofacial soft-tissue injury

Tanaka

Hamada

Yokoyama

et al. 2021

Japanese Dental Science Review

View full text Add to dashboard Cite

Synthetic peptide SVVYGLR upregulates cell motility and facilitates oral mucosal wound healing

Cited by 12 publications

References 27 publications

The synthetic peptide SVVYGLR promotes myogenic cell motility <i>via</i> the TGFβ1/Smad signaling pathway and facilitates skeletal myogenic differentiation <i>in vitro </i>

The synthetic peptide SVVYGLR promotes myogenic cell motility <i>via</i> the TGFβ1/Smad signaling pathway and facilitates skeletal myogenic differentiation <i>in vitro </i>

Bioactive Synthetic Peptides for Oral Tissues Regeneration

Osteopontin-derived synthetic peptide SVVYGLR upregulates functional regeneration of oral and maxillofacial soft-tissue injury

Contact Info

Product

Resources

About