Synthetic peptides as an antigenic base in an ELISA for laboratory diagnosis of schistosomiasis mansoni

Abstract

A pool of five synthetic peptides was used as an antigenic base in an ELISA (ELISA-Pp) for laboratory diagnosis of Schistosoma mansoni. Serum samples were obtained from individuals with acute (n=23) and chronic (n=30) schistosomiasis, with other parasitoses (n=39) or without parasitic infections (n=100). ELISA-Pp was compared with other immunoenzymatic methods for detection of IgM (IgM-ELISA) or IgG (IgG-ELISA) as well as an immunofluorescence test for detection of IgM antibodies (IgM-IFT). The sensitivity and specificity of ELISA-Pp was 86.8% and 94.2% when tested on the schistosomiasis group and the non-schistosomiasis group, respectively. Comparison of ELISA-Pp with other serological methods resulted in kappa concordance indices varying from 0.59 to 0.75. Evaluation of anti-peptide IgG antibodies showed higher levels in patients with acute compared with chronic schistosomiasis (P=0.001). ELISA-Pp showed satisfactory sensitivity and high specificity and may constitute a potentially useful method for laboratory diagnosis of schistosomiasis mansoni.

Cysticercus cellulosae antigens in the serodiagnosis of neurocysticercosis

“…[75] Subsequently, the synthetic polypeptides that represented the full-length mature protein sTS14 were assessed for serologic potential using ELISA for the diagnosis of NCC. [76] Since then, pure epitopes and molecules are being increasingly used as diagnostic antigens in a large number of parasitic infections including malaria,[77] Chagas’ disease,[78] leishmaniasis,[79] schistosomiasis,[80] cystic echinococcosis,[81] fascioliasis,[82] and cysticercosis. [83]…”

Epitopes rationally selected through computational analyses induce T‐cell proliferation in mice and are recognized by serum from individuals infected with Schistosoma mansoni

“…Oliveira and colleagues have used a pool of epitopes from S. mansoni proteins in ELISA assays and demonstrated the high efficacy of the test when compared to adult worm extract or soluble antigens, with a similar sensitivity to that observed using whole antigens. Epitopes selected through computational analyses have been used to compose a multiepitopic chimeric protein improving the immune response in vaccine assays or the sensitivity of detection in serological diagnosis.…”

New Approaches with Different Types of Circulating Cathodic Antigen for the Diagnosis of Patients with Low Schistosoma mansoni Load

“…However, fluctuation in egg output and the chance of missing light infections necessitate repeated examinations [9]. Serologic testing has been used to enhance our ability to detect the disease in order to be more sensitive in demonstrating light infections [10]–[12], [38]. This study applied a multievaluation approach, combining specific antibody detection for four different antigens with an investigation performed on parasitological data in efforts to produce a more field-applicable assay format.…”