Synthetic peptides cytomodulin-1 (CM-1) and cytomodulin-2 (CM-2) promote collagen synthesis and wound healing in vitro

Abstract

Transforming growth factor-beta1 (TGF-beta1) is believed to be a key player in wound healing, promoting cell proliferation, migration, and matrix synthesis in a variety of cell types. We have designed two peptides, i.e., cytomodulin-1 (CM-1) and cytomodulin-2 (CM-2), to simulate the binding domain of TGF-beta1. In this study we examined the bioactivity of the two synthetic peptides CM-1 and CM-2 on human foreskin fibroblasts (HFF). Synthetic peptides CM-1 and CM-2 in culture media increased wound healing of fibroblasts in an injury model in vitro. In addition, CM-1 and CM-2 enhanced the gene expression of collagen I and increased the production of pro-collagen I peptide in HFF. These results suggest that CM-1 and CM-2 have potentially useful clinical applications in wound healing.

A new, bioactive, antibacterial‐eluting, composite graft for infection‐free wound healing

“…As reported earlier by our group, cytomodulin immobilized onto the porous PLGA scaffolds resulted in better cell migration, actin production, and wound healing in rodent model . Cytomodulin is a synthetic, tumor growth factor beta (TGF‐β) mimic and acts as a substitute for cytokines in many fields particularly wound healing and skin regeneration and thus create a natural habitat within the scaffolds . Covalent immobilization of such biomimetic molecules or growth factors onto the scaffolds enabled single application by preventing its rapid degradation …”

“…Further, the composite graft treated groups exhibited significantly higher collagen content on day 7 and this might be attributed to the presence of TGF‐β mimic cytomodulin on the porous scaffolds in the composite graft. It has been reported that cytomodulin when added to the cells in cultures of neonatal human dermal fibroblasts induced increased expression of type I collagen . Gradually, as the granulation tissue is replaced by the scar tissue, the production of collagen is down‐regulated and collagen starts forming dense and compact bundles as seen in the Masson's Trichrome stained sections.…”

Cytomodulin-functionalized porous PLGA particulate scaffolds respond better to cell migration, actin production and wound healing in rodent model

“…This signifies the role of biomimetic peptides for enabling better and more effective wound healing. The exact molecular mechanism by which CM interacts with the cells is not known; however, it has been reported that the stable bend structure of cytomodulin (−Val–Ala–) interacts with cell surface receptors (TGF β ) (Lam et al ., ) and this might be the reason for the observed responses in PMS_CM. In the present study, PMS_CM scaffold provided (−Val–Ala–) bend structure, which interacted with the cells via their TGF β receptors and thus enhanced collagen production, cell migration, actin production and, hence, wound healing.…”

“…Research on this biomimetic molecule is at an early stage; however, some analogues of cytomodulin, e.g. CM‐1 and CM‐2, were found effective to enhance the expression of collagen I and pro‐collagen I in wound healing of fibroblasts in an in vitro injury model (Lam et al ., ); another study by Basu et al . () has reported CM‐10 to be a strong promoter of wound healing (by primary intention).…”

“…Research on this biomimetic molecule is at an early stage; however, some analogues of cytomodulin, e.g. CM-1 and CM-2, were found effective to enhance the expression of collagen I and pro-collagen I in wound healing of fibroblasts in an in vitro injury model (Lam et al, 2004); another study by Basu et al (2009) has reported CM-10 to be a strong promoter of wound healing (by primary intention). In the present study, the objective was to enhance the effectiveness of scaffolds by coupling cytomodulin and using them for the healing of fullthickness wounds by secondary intention.…”