Synthetic Peptides Derived from Ribosomal Proteins of Leishmania spp. in Mucocutaneous Leishmaniasis: Diagnostic Usefulness

Flórez, Magda Melissa; Puerta, Concepción J.; Guzmán, Fanny; Ayala, Martha S.; Montoya, Gladis; Delgado, Gabriela

doi:10.2174/0929866524666170728143924

Cited by 4 publications

(2 citation statements)

References 21 publications

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“…In parasites of medical importance, as several species of Leishmania, a relationship between the sequence conservation and the diagnosis potential of some evolutionarily conserved proteins has been established [16]. In this context, we explored the diagnosis potential of the conserved Tc964 protein.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Tc964, A Novel Antigenic Protein from Trypanosoma cruzi

Ruiz-Márvez

Ramírez

Rodríguez

et al. 2020

IJMS

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The Tc964 protein was initially identified by its presence in the interactome associated with the LYT1 mRNAs, which code for a virulence factor of Trypanosoma cruzi. Tc964 is annotated in the T. cruzi genome as a hypothetical protein. According to phylogenetic analysis, the protein is conserved in the different genera of the Trypanosomatidae family; however, recognizable orthologues were not identified in other groups of organisms. Therefore, as a first step, an in-depth molecular characterization of the Tc946 protein was carried out. Based on structural predictions and molecular dynamics studies, the Tc964 protein would belong to a particular class of GTPases. Subcellular fractionation analysis indicated that Tc964 is a nucleocytoplasmic protein. Additionally, the protein was expressed as a recombinant protein in order to analyze its antigenicity with sera from Chagas disease (CD) patients. Tc964 was found to be antigenic, and B-cell epitopes were mapped by the use of synthetic peptides. In parallel, the Leishmania major homologue (Lm964) was also expressed as recombinant protein and used for a preliminary evaluation of antigen cross-reactivity in CD patients. Interestingly, Tc964 was recognized by sera from Chronic CD (CCD) patients at different stages of disease severity, but no reactivity against this protein was observed when sera from Colombian patients with cutaneous leishmaniasis were analyzed. Therefore, Tc964 would be adequate for CD diagnosis in areas where both infections (CD and leishmaniasis) coexist, even though additional assays using larger collections of sera are needed in order to confirm its usefulness for differential serodiagnosis.

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Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Tc964, A Novel Antigenic Protein from Trypanosoma cruzi

Ruiz-Márvez

Ramírez

Rodríguez

et al. 2020

IJMS

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“…Since CL-patients commonly produce low levels of anti-Leishmania antibodies, there is growing interest in high sensitivity antigens for immunological tests. Different methodologies have been employed, such as bioinformatics tools [19][20][21][22][23], cDNA expression library [24], phage display [25,26], immunoproteomic approach [18,[27][28][29][30][31][32] and isolation and purification of glycoconjugates [33,34] to identify potential antigens. Furthermore, immunological tools have already been used to detect Leishmania antigens using monoclonal and polyclonal antibodies by immunochromatographic test (ICT) or immunohistochemistry (IHC), such as the CL Detect Rapid Test (InBios International Inc., Seattle, WA, USA), which detects peroxidoxin from Leishmania and has been used especially in Old World countries, with limited sensitivity [35,36].…”

Section: Introductionmentioning

confidence: 99%

Potential antigenic targets used in immunological tests for diagnosis of tegumentary leishmaniasis: A systematic review

et al. 2021

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Immunological tests may represent valuable tools for the diagnosis of human tegumentary leishmaniasis (TL) due to their simple execution, less invasive nature and potential use as a point-of-care test. Indeed, several antigenic targets have been used with the aim of improving the restricted scenario for TL-diagnosis. We performed a worldwide systematic review to identify antigenic targets that have been evaluated for the main clinical forms of TL, such as cutaneous (CL) and mucosal (ML) leishmaniasis. Included were original studies evaluating the sensitivity and specificity of immunological tests for human-TL, CL and/or ML diagnosis using purified or recombinant proteins, synthetic peptides or polyclonal or monoclonal antibodies to detect Leishmania-specific antibodies or antigens. The review methodology followed PRISMA guidelines and all selected studies were evaluated in accordance with QUADAS-2. Thirty-eight original studies from four databases fulfilled the selection criteria. A total of 79 antigens were evaluated for the detection of antibodies as a diagnostic for TL, CL and/or ML by ELISA. Furthermore, three antibodies were evaluated for the detection of antigen by immunochromatographic test (ICT) and immunohistochemistry (IHC) for CL-diagnosis. Several antigenic targets showed 100% of sensitivity and specificity, suggesting potential use for TL-diagnosis in its different clinical manifestations. However, a high number of proof-of-concept studies reinforce the need for further analysis aimed at verifying true diagnostic accuracy in clinical practice.

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Recent advances and new strategies in Leishmaniasis diagnosis

Brito

Aguiar-Soares

Cardoso

et al. 2020

Appl Microbiol Biotechnol

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Synthetic Peptides Derived from Ribosomal Proteins of Leishmania spp. in Mucocutaneous Leishmaniasis: Diagnostic Usefulness

Cited by 4 publications

References 21 publications

Molecular Characterization of Tc964, A Novel Antigenic Protein from Trypanosoma cruzi

Molecular Characterization of Tc964, A Novel Antigenic Protein from Trypanosoma cruzi

Potential antigenic targets used in immunological tests for diagnosis of tegumentary leishmaniasis: A systematic review

Recent advances and new strategies in Leishmaniasis diagnosis

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