Synthetic Phosphoethanolamine Induces Apoptosis Through Caspase-3 Pathway by Decreasing Expression of Bax/Bad Protein and Changes Cell Cycle in Melanoma

Ferreira, Adilson Kleber; Meneguelo, Renato; Neto, Salvador Claro; Chierice, Gilberto Orivaldo; Maria, Durvanei Augusto

doi:10.4172/1948-5956.1000058

Cited by 47 publications

(97 citation statements)

References 26 publications

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“…Follicular carcinoma is more common in women who are affected over a broad age range [21].Cancer is basically a cell disease, characterized by a loss in the mechanisms which drive the proliferation and cellular differentiation [22]. The cells were cultured and the proliferation was assessed by cell counting using a haemocytometer after trypsin digestion [23].…”

Section: Follicular Thyroid Cancermentioning

confidence: 99%

Epidemiology and Treatment for Thyroid Cancer

Srilatha¹,

A²,

Soumya³

2011

J Cancer Sci Ther

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Section: Follicular Thyroid Cancermentioning

confidence: 99%

Epidemiology and Treatment for Thyroid Cancer

Srilatha¹,

A²,

Soumya³

2011

J Cancer Sci Ther

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“…Dacarbazine is currently the only approved chemotherapy drug for the standard treatment of metastatic melanoma stage IV, but its therapeutic response is not satisfactory and only 15-20% of patients with favorable prognosis have increased survival rate [68].…”

Section: Drugsmentioning

confidence: 99%

A Glimpse on Melanoma - Risk Factors and Treatment

Soumya¹

2011

J Cancer Sci Ther

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Melanoma is the most dreadful form of skin cancer that begins in melanocytes. It is the leading cause of death from skin disease. It can occur in any part of the body that contains melanocytes. Several internal and external risk factors of the body may contribute to the development of many cancer melanomas such as unexpected exposure to UV radiation, weakened immune system, family history etc. Melanoma diagnosed at early stages is typically cured by surgical excision, but advanced stages are often deadly. Cancer stem cells (CSC) are recently proposed to be the cancer initiating cells responsible for tumorigenesis and contribute to cancer resistance. In the present review I have discussed about melanoma incidence, detection with various modes of treatment.

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“…2,3 In a recent report, we showed that synthetic phosphoethanolamine (PHO-S), an aminoethyl phosphoric ester, has a great potential to induce cell death in B16F10 (melanoma murine) cells and without apparent damage to normal cells, such as fibroblasts and lymphocytes. 4,5 We demonstrated that PHO-S is effective in the reduction of DNA…”

Section: Introductionmentioning

confidence: 97%

“…[4][5][6][7] The antineoplastic phospholipids are a new class of antitumor agents, which can cause collateral effects, such as gastrointestinal toxicity, hemolysis, and liver and renal dysfunction. These effects are attributed to agents such as edelfosine, ilmofosine, and miltefosine.…”

mentioning

confidence: 99%

Potential antitumor activity of novel DODAC/PHO-S liposomes

Luna

Saraiva²,

Filho³

et al. 2016

IJN

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In recent studies, we showed that synthetic phosphoethanolamine (PHO-S) has a great potential for inducing cell death in several tumor cell lines without damage to normal cells. However, its cytotoxic effect and selectivity against tumor cells could increase with encapsulation in cationic liposomes, such as dioctadecyldimethylammonium chloride (DODAC), due to electrostatic interactions between these liposomes and tumor cell membranes. Our aim was to use cationic liposomes to deliver PHO-S and to furthermore maximize the therapeutic effect of this compound. DODAC liposomes containing PHO-S (DODAC/PHO-S), at concentrations of 0.3–2.0 mM, prepared by ultrasonication, were analyzed by scanning electron microscopy (SEM) and dynamic light scattering. The cytotoxic effect of DODAC/PHO-S on B16F10 cells, Hepa1c1c7 cells, and human umbilical vein endothelial cells (HUVECs) was assessed by MTT assay. Cell cycle phases of B16F10 cells were analyzed by flow cytometry and the morphological changes by SEM, after treatment. The liposomes were spherical and polydisperse in solution. The liposomes were stable, presenting an average of ∼50% of PHO-S encapsulation, with a small reduction after 40 days. DODAC demonstrated efficient PHO-S delivery, with the lowest values of IC 50% (concentration that inhibits 50% of the growth of cells) for tumor cells, compared with PHO-S alone, with an IC 50% value of 0.8 mM for B16F10 cells and 0.2 mM for Hepa1c1c7 cells, and without significant effects on endothelial cells. The Hepa1c1c7 cells showed greater sensitivity to the DODAC/PHO-S formulation when compared to B16F10 cells and HUVECs. The use of DODAC/PHO-S on B16F10 cells induced G 2 /M-phase cell cycle arrest, with the proportion significantly greater than that treated with PHO-S alone. The morphological analysis of B16F10 cells by SEM showed changes such as “bleb” formation, cell detachment, cytoplasmic retraction, and apoptotic bodies after DODAC/PHO-S treatment. Cationic liposomal formulation for PHO-S delivery promoted cytotoxicity more selectively and effectively against B16F10 and Hepa1c1c7 cells. Thus, the DODAC/PHO-S liposomal formulation presents great potential for preclinical studies.

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Synthetic Phosphoethanolamine Induces Apoptosis Through Caspase-3 Pathway by Decreasing Expression of Bax/Bad Protein and Changes Cell Cycle in Melanoma

Cited by 47 publications

References 26 publications

Epidemiology and Treatment for Thyroid Cancer

Epidemiology and Treatment for Thyroid Cancer

A Glimpse on Melanoma - Risk Factors and Treatment

Potential antitumor activity of novel DODAC/PHO-S liposomes

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