Synthetic Polymer Scaffolds for Stem Cell Transplantation in Retinal Tissue Engineering

Yao, Jing; Tao, Sarah L.; Young, Michael J.

doi:10.3390/polym3020899

Cited by 56 publications

(42 citation statements)

References 80 publications

(131 reference statements)

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“…Cryogels typically exhibit enhanced mechanical stability with respect to traditional hydrogels (17). Recent studies have shown the potential application of cryogels as tissue engineering scaffolds (19)(20)(21).Due to the trauma resulting from surgical implantation of 3D scaffolds, minimally invasive surgical approaches to deliver polymeric scaffolds have been the focus of recent work (22,23). For a biomaterial to be injectable, it has to be able to flow through a small-bore needle or a catheter.…”

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confidence: 99%

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Injectable preformed scaffolds with shape-memory properties

Bencherif

Sands

Bhatta

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

441

407

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Injectable biomaterials are increasingly being explored to minimize risks and complications associated with surgical implantation. We describe a strategy for delivery via conventional needle-syringe injection of large preformed macroporous scaffolds with well-defined properties. Injectable 3D scaffolds, in the form of elastic sponge-like matrices, were prepared by environmentally friendly cryotropic gelation of a naturally sourced polymer. Cryogels with shape-memory properties may be molded to a variety of shapes and sizes, and may be optionally loaded with therapeutic agents or cells. These scaffolds have the capability to withstand reversible deformations at over 90% strain level, and a rapid volumetric recovery allows the structurally defined scaffolds to be injected through a small-bore needle with nearly complete geometric restoration once delivered. These gels demonstrated long-term release of biomolecules in vivo. Furthermore, cryogels impregnated with bioluminescent reporter cells provided enhanced survival, higher local retention, and extended engraftment of transplanted cells at the injection site compared with a standard injection technique. These injectable scaffolds show great promise for various biomedical applications, including cell therapies.syringe-injectable hydrogels | cryogelation | shape retention | controlled delivery | cell therapy H ydrogels are 3D networks that can absorb a large amount of water while maintaining their structural integrity. They are considered as appropriate scaffolds for tissue engineering applications mainly due to their structural similarities with native soft tissue, and are widely used as soft materials in many biomedical applications including cell culture, cell encapsulation, controlled delivery of therapeutic agents, and medical device fabrication (1-6).Hydrogels typically exhibit a nanoporous network structure, but it is advantageous to use hydrophilic networks with large interconnected pores (>10 μm) to allow cell infiltration and deployment, and provide an increased surface area for cell attachment and interaction. As a result, macroporous hydrogel scaffolds have been developed using various techniques such as fiber bonding, gas foaming, microemulsion formation, phase separation, freeze-drying, and porogen leaching (7-15). More recently, gelation at subzero temperatures, known as cryogelation, has been used to create hydrogels with large interconnected pores (16-18). During cryogelation, the reactants are restricted to the unfrozen/semifrozen phases and form a cross-linked network upon polymerization, while the ice crystals nucleated from the aqueous phase during freezing function as porogens. The melting of these ice crystals at temperature above the freezing temperature gives rise to interconnected macroporous networks. Cryogels typically exhibit enhanced mechanical stability with respect to traditional hydrogels (17). Recent studies have shown the potential application of cryogels as tissue engineering scaffolds (19)(20)(21).Due to the trauma resulting fro...

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confidence: 99%

“…Due to the trauma resulting from surgical implantation of 3D scaffolds, minimally invasive surgical approaches to deliver polymeric scaffolds have been the focus of recent work (22,23). For a biomaterial to be injectable, it has to be able to flow through a small-bore needle or a catheter.…”

mentioning

confidence: 99%

Injectable preformed scaffolds with shape-memory properties

Bencherif

Sands

Bhatta

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

441

407

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“…Cell delivery systems Scaffolds for cell delivery have been designed to promote cell survival and integration [203,204] by protecting cells from the hostile host environment [205,206]. Scaffolds can support uniform cell distribution and direct the differentiation of transplanted stem cell progeny [207].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Delivery strategies for treatment of age-related ocular diseases: From a biological understanding to biomaterial solutions

Delplace

Payne

Shoichet

2015

Journal of Controlled Release

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“…Matrix metalloproteinase 2 (MMP2) can promote host-donor integration and thus copoly- mers of PLGA/MMP2 were developed to enhance cell integration and promote retinal repopulation [15]. Delivery of RPCs to dystrophic Rho -/retinas using MMP2-PLGA electrospun fibrous scaffolds leads to enhanced expression of the photoreceptor markers and controlled release of MMP2 [15,55]. Another synthetic polymer initially explored for subretinal implantation of RPCs was polymethylmethacrylate (PMMA).…”

Section: Substrates and Scaffolds For Retinal Cell Deliverymentioning

confidence: 99%

Approaches to Cell Delivery: Substrates and Scaffolds for Cell Therapy

Kundu

Michaelson²,

Baranov³

et al. 2014

Developments in Ophthalmology

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Retinal degeneration, associated with loss of photoreceptors, is the primary cause of permanent vision impairment, impacting millions of people worldwide. Age-related macular degeneration and retinitis pigmentosa are two common retinal diseases resulting in photoreceptor loss and vision impairment or blindness. Presently, available treatments can only delay the progress of retinal degeneration, and there are no treatments that can restore permanent vision loss. Research is underway to develop methods of regenerating the impaired retina by delivering photoreceptor precursor cells and retinal pigment epithelium to the subretinal space. Challenges to cell transplantation include limited survival upon implantation and the formation of abnormal cell architectures in vivo. Retinal tissue engineering shows immense promise and potential in treatment of retinal degeneration by employing scaffold-based delivery systems of retinal progenitor cells to the subretinal space. Scaffold delivery strategy has been shown to enhance the cell survival and direct cell differentiation in a variety of retinal degenerative models. In this chapter, we summarize the research findings on different scaffold- or substrate-based transplantation techniques used to deliver retinal progenitor/photoreceptor precursors and retinal pigment epithelial cells to the subretinal space.

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Synthetic Polymer Scaffolds for Stem Cell Transplantation in Retinal Tissue Engineering

Cited by 56 publications

References 80 publications

Injectable preformed scaffolds with shape-memory properties

Injectable preformed scaffolds with shape-memory properties

Delivery strategies for treatment of age-related ocular diseases: From a biological understanding to biomaterial solutions

Approaches to Cell Delivery: Substrates and Scaffolds for Cell Therapy

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