Synthetic polymeric variant of S-adenosyl methionine synthetase

Datta, Lakshmi Priya; Maiti, Binoy; De, Priyadarsi

doi:10.1039/c5py01230a

Cited by 24 publications

(24 citation statements)

References 27 publications

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“…The alanine-based vinyl monomer, Boc- l -Ala-HEMA, was synthesized by the esterification reaction between HEMA and Boc- l -Ala-OH, according to the procedure reported for the synthesis of amino acid-based vinyl monomers. , Herein, ethyl acetate was used as the coupling solvent for the esterification reaction. The condensation reaction was carried out in the presence of dicyclohexylcarbodiimide (DCC) as the coupling agent and 4-dimethylaminopyridine (DMAP) as the catalyst.…”

Section: Methodsmentioning

confidence: 99%

Peptide-Based Polymer–Polyoxometalate Supramolecular Structure with a Differed Antimicrobial Mechanism

et al. 2017

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Because of the increasing prevalence of multidrug resistance feature, several investigations have been so far reported regarding the antibiotic alternative supramolecular bioactive agents made of hybrid assemblies. In this regard, it is well-established that combinational therapy inherited by assembled supramolecular structures can improve the bioactivity to some extent, but their mode of action has not been studied in detail. We provide first direct evidence that the improved mechanism of action of antimicrobial supra-amphiphilic nanocomposites differs largely from their parent antimicrobial peptide-based polymers. For the construction of a hybrid combinational system, we have synthesized side-chain peptide-based antimicrobial polymers via RAFT polymerization and exploited their cationic nature to decorate supra-amphiphilic nanocomposites via interaction with anionic polyoxometalates. Because of cooperative antimicrobial properties of both the polymer and polyoxometalate, the nanocomposites show an enhanced antimicrobial activity with a different antimicrobial mechanism. The cationic stimuli-responsive peptide-based polymers attack bacteria via membrane disruption mechanism, whereas free radical-mediated cell damage is the likely mechanism of polymer-polyoxometalate-based supra-amphiphilic nanocomposites. Thus, our study highlights the different antimicrobial mechanism of combinational systems in detail, which improves our understanding of enhanced antimicrobial efficacy.

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Section: Methodsmentioning

confidence: 99%

Peptide-Based Polymer–Polyoxometalate Supramolecular Structure with a Differed Antimicrobial Mechanism

et al. 2017

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“…Synthesis of a polyampholyte copolymer with a well-defined and regular structure can be achieved by the reversible addition–fragmentation chain transfer (RAFT) polymerization technique . The incorporation of biological moieties within an artificial or synthetic polymeric system is of particular interest to integrate the biomimetic features. − Herein, we employed RAFT polymerization protocols to synthesize a block copolymer containing two polymethacrylate blocks with carboxylic side chains in one of the blocks functionalized with tryptophan (Trp). The incorporation of Trp units at the side chains allows for the utilization of free amine and indole groups to infuse polyampholytic character to the block copolymer.…”

Section: Introductionmentioning

confidence: 99%

Polyampholyte-Based Synthetic Chaperone Modulate Amyloid Aggregation and Lithium Delivery

Datta

Samanta

Govindaraju

2020

ACS Chem. Neurosci.

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Protein misfolding and aggregation is the pathological hallmark of Alzheimer’s disease (AD). The etiopathogenesis of AD involves the accumulation of amyloid-β (Aβ) plaques in the brain, which disrupt the neuronal network and communication, causing neuronal death and severe cognitive impairment. Modulation of Aβ aggregation by exogenous therapeutic agents is considered an effective strategy to treat AD. Frequent failure of drug candidates in various phases of clinical trials reiterates the need for alternative therapeutic strategies for AD treatment. Polyampholytes with cationic and anionic segments are considered as artificial protein mimics capable of modulating the protein misfolding and aggregation. We report a diblock copolymer of tryptophan-functionalized methacrylic acid (PTMA) polyampholyte synthesized through reversible addition–fragmentation chain transfer (RAFT) polymerization. Investigation revealed that PTMA acts as a synthetic chaperone to protect the native structure of the lysozyme under heat-induced aggregation conditions. PTMA effectively modulates Aβ aggregation and rescues neuronal cells. Lithium has been shown to exhibit therapeutic efficacy in chronic neurological diseases including AD. PTMA sequesters and releases lithium ions in response to neuropathological pH stimuli, making it a promising candidate for lithium transport and delivery. The detailed studies demonstrate PTMA as aggregation modulator and lithium carrier with implications for combinational therapy to treat AD.

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“…Apart from the above widespread applications, self-assembled amino-acid-based polymeric nanostructures have also shown enormous potential in several other clinical applications such as biosensing, bioimaging, mimicking enzymatic activity, 100 inhibiting fibrillization of proteins meant for neurological and nonneuropathic disorders etc. 101 Recently, our group has explored the applications of side-chain proline-based polymers in inhibiting the formation of insulin fibrils, which is considered a hallmark of diabetes.…”

Section: Other Bioapplicationsmentioning

confidence: 99%

Side‐chain amino‐acid‐based polymers: self‐assembly and bioapplications

Nayak

Ghosh

Khan

et al. 2021

Polymer International

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The design and development of side-chain amino-acid-based polymeric nanostructures have attracted a significant research interest as they bring a remarkable revolution in various territories of the biomedical field. The incorporation of amino acid moieties into the side chain of synthetic polymeric scaffolds exhibits several beneficial properties like aqueous solubility, chiral recognition, high biocompatibility, stimuli responsiveness, antifouling properties, as well as their capability to form higher ordered self-assembled architectures. Considering the important features and widespread applications of side-chain aminoacid-containing polymers, here we shed light on the self-assembled characteristics of side-chain amino-acid-containing polymers and their implications. The primary aim of this review article is to highlight the recent achievements of this bright area of research. We describe the numerous aspects of side-chain amino-acid-containing polymers focusing mainly on self-assembly properties and the biomedical applications, which include drug and gene delivery, antimicrobial activity, antifouling coating, wound healing, tissue engineering etc.

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Synthetic polymeric variant of S-adenosyl methionine synthetase

Abstract: Side-chain l-methionine containing polymers show the ability to donate a methyl group in the absence of a methyltransferase enzyme, which confirms their enzyme-like activity.

Cited by 24 publications

References 27 publications

Peptide-Based Polymer–Polyoxometalate Supramolecular Structure with a Differed Antimicrobial Mechanism

Peptide-Based Polymer–Polyoxometalate Supramolecular Structure with a Differed Antimicrobial Mechanism

Polyampholyte-Based Synthetic Chaperone Modulate Amyloid Aggregation and Lithium Delivery

Side‐chain amino‐acid‐based polymers: self‐assembly and bioapplications

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