Synthetic Promoters from Strawberry Vein Banding Virus (SVBV) and Dahlia Mosaic Virus (DaMV)

Khadanga, Badrinath; Chanwala, Jeky; Sandeep, I. Sriram; Dey, Nrisingha

doi:10.1007/s12033-021-00344-5

Cited by 4 publications

(4 citation statements)

References 52 publications

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“…The S100 recombinant promoter (621 bp) was obtained from the 250bp long upstream activation sequence (UAS) of the strawberry vein banding virus (SV10UAS; −352 to 102 relative to TSS) and the 371bp long TATA-containing core promoter domain (SV10CP; −352 to +19) of the same gene. Correspondingly, the 726bplong D100 promoter was assembled by fusion of the 170bp long UAS of the dahlia mosaic virus (DaMV14UAS; −203 to−33) with its 556-bp-long core promoter domain (DaMV4CP; −474 to +82) [231].…”

Section: Cis-element Juggling and Domain Swappingmentioning

confidence: 99%

Plant Synthetic Promoters

Szymczyk,

Majewska

2024

Applied Sciences

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This article examines the structure and functions of the plant synthetic promoters frequently used to precisely regulate complex regulatory routes. It details the composition of native promoters and their interacting proteins to provide a better understanding of the tasks associated with synthetic promoter development. The production of synthetic promoters is performed by relatively small libraries produced generally by basic molecular or genetic engineering methods such as cis-element shuffling or domain swapping. The article also describes the preparation of large-scale libraries supported by synthetic DNA fragments, directed evolution, and machine or deep-learning methodologies. The broader application of novel, synthetic promoters reduces the prevalence of homology-based gene silencing or improves the stability of transgenes. A particularly interesting group of synthetic promoters are bidirectional forms, which can enable the expression of up to eight genes by one regulatory element. The introduction and controlled expression of several genes after one transgenic event strongly decreases the frequency of such problems as complex segregation patterns and the random integration of multiple transgenes. These complications are commonly observed during the transgenic crop development enabled by traditional, multistep transformation using genetic constructs containing a single gene. As previously tested DNA promoter fragments demonstrate low complexity and homology, their abundance can be increased by using orthogonal expression systems composed of synthetic promoters and trans-factors that do not occur in nature or arise from different species. Their structure, functions, and applications are rendered in the article. Among them are presented orthogonal systems based on transcription activator-like effectors (dTALEs), synthetic dTALE activated promoters (STAPs) and dCas9-dependent artificial trans-factors (ATFs). Synthetic plant promoters are valuable tools for providing precise spatiotemporal regulation and introducing logic gates into the complex genetic traits that are important for basic research studies and their application in crop plant development. Precisely regulated metabolic routes are less prone to undesirable feedback regulation and energy waste, thus improving the efficiency of transgenic crops.

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Section: Cis-element Juggling and Domain Swappingmentioning

confidence: 99%

Plant Synthetic Promoters

Szymczyk,

Majewska

2024

Applied Sciences

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“…The S100 recombinant promoter (621 bp) was obtained from the 250-bp-long upstream activation sequence (UAS) of the Strawberry Vein Banding Virus (SV10UAS; − 352 to − 102 relative to TSS) and the 371-bp-long TATA-containing core promoter domain (SV10CP; − 352 to +19) of the same gene. Correspondingly, the 726-bp-long D100 promoter was assembled by fusion of the 170-bp-long UAS of the Dahlia Mosaic Virus (DaMV14UAS; − 203 to − 33) with its 556-bp-long core promoter domain (DaMV4CP; − 474 to +82) [231].…”

Section: Cis-elements Juggling and Domain Swappingmentioning

confidence: 99%

Plant Synthetic Promoters

Szymczyk,

Majewska

2024

Preprint

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The article features the structure and functions of plant synthetic promoters frequently exercised to precisely regulate complex regulatory routes. The composition of plant native promoters together with interacting proteins is presented to provide a better understanding of tasks associated with synthetic promoter development. The production of synthetic promoters is conferred on relatively small libraries produced generally by basic molecular or genetic engineering methods such as cis-element shuffling or domain swapping. Moreover, the preparation of large-scale libraries supported by synthetic DNA fragments, directed evolution, and machine or deep learning methodologies is presented. A particularly interesting group of synthetic promoters are bidirectional forms that enable the putative expression of up to 6–8 genes by one regulatory element. The introduction and controlled expression of several genes after one transgenic event strongly decreases the frequency of such problems as complex segregation patterns and random integration of multiple transgenes. These complications are commonly observed during transgenic crop development through traditional, multistep transformation by genetic constructs containing a single gene. Another path to solving problems associated with the low complexity and homology of already tested DNA fragments is through orthogonal expression systems composed of synthetic promoters and trans-factors that do not occur in nature or arise from different species. Their structure, functions, and applications are rendered in the article.

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“…(2021) MiniSyn CREs from plants artificially synthesized constitutive expression tobacco, oilseed rape Jores et al. (2021) S100 D100 strawberry vein banding virus, dahlia mosaic virus element shuffling abscisic acid, salicylic acid induced antifungal, antibacterial activity tobacco Khadanga et al. (2021) BL1 BL2 ERF53 , RD29A minimal promoter manually synthesized stress inducible abiotic stress Arabidopsis Kim et al.…”

Section: Strategy Of Synthetic Promoter Designmentioning

confidence: 99%

“…To decrease transgene silencing that results from repetitive use of a single promoter, synthetic promoters can combine functionally equivalent cis elements from diverse non-plant organisms, thus maximizing sequence diversity ( Kumar et al., 2012 , 2015 ; Gupta et al., 2021 ; Khadanga et al., 2021 ; Sethi et al., 2021 ). Hybrid promoters using the components of two viruses had 2.5 times higher activity than the CaMV35S promoter in prokaryotes and plants ( Gupta et al., 2021 ).…”

Section: Limitations Overcome By Synthetic Promoters In the Control O...mentioning

confidence: 99%