Synthetic seed production of medicinal plants: a review on influence of explants, encapsulation agent and matrix

Gantait, Saikat; Kundu, Suprabuddha; Ali, Md. Nasim; Sahu, Narayan Chandra

doi:10.1007/s11738-015-1847-2

Cited by 113 publications

(47 citation statements)

References 87 publications

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“…Plant germplasm can generally be preserved, as synthetic seeds, by encapsulating small explant such as shoot tips, nodes, or axillary buds in calcium alginate [53,[186][187][188][189][190][191]. Encapsulation can limit the size of the shoots, especially when the synthetic seeds are preserved under minimal-growth conditions of low temperature, reduced irradiance, or decreased nutrient supply [56,178,[192][193][194][195][196].…”

Section: In Vitro Preservationmentioning

confidence: 99%

Tissue Culture of Corymbia and Eucalyptus

Trueman

Hung

Wendling

2018

Forests

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Eucalypts are among the world's most widely planted trees, but the productivity of eucalypt plantations is limited by their often-low amenability to true-to-type propagation from cuttings. An alternative approach to cutting propagation is tissue culture, which can be used to micropropagate valuable genotypes rapidly while simultaneously preserving germplasm in vitro. This review describes the use of tissue culture methods such as shoot culture, organogenesis, and somatic embryogenesis for micropropagating eucalypts. This review also discusses the use of cool storage, encapsulation, and cryopreservation methods for preserving eucalypt germplasm and delaying tissue maturation under minimal-growth conditions.

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Section: In Vitro Preservationmentioning

confidence: 99%

Tissue Culture of Corymbia and Eucalyptus

Trueman

Hung

Wendling

2018

Forests

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“…Certain compounds like-DMSO (dimethyl sulfoxide), glycerol, ethylene, propylene, sucrose, mannose, glucose, praline, acetamide are added during the cryopreservation. These are called cryoprotectants and prevent the damage caused to cells by reducing the freezing point and super cooling point of water., (Ara et al, 2000;Sharma et al, 2013a;Perveen and Anis, 2014;Gantait et al, 2015)…”

Section: Conservation Of Swertia Chirayitamentioning

confidence: 99%

“…Developing an in vitro regeneration protocol for Swertia chirayita is urgent to promote large-scale production for ex situ conservation and for satisfying the pharmaceutical needs. Synthetic seed technology is also an applied application of modern plant biotechnology which offers tremendous potential for easy handling, micropropagation and plant germplasm conservation through cryopreservation (Gantait et al 2015 andStaden, 2016).…”

Section: Introductionmentioning

confidence: 99%

A mini review on in vitro propagation of Swertia chirayita an endangered medicinal plant

Shailja¹

2017

Biosci. Biotech. Res. Comm

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Swertia chirayita is an endangered indigenous medicinal herb. It is used in traditional medicine to treat various ailments. Swertia chirayita is found in temperate regions of the Himalaya at an altitude of 1200-3000 m from Kashmir to Nepal, Bhutan and grows in the slopes of moist shady places. The species is valued for its bitterness. The bitterness, antihelmintic, hypoglycemic and antipyretic properties are attributed to amarogentin, swerchirin, swertiamarin and other active principles of the herb. Its medicinal usage is reported in Indian pharmaceutical codex, the British and the American pharmacopoeias and in different traditional systems of medicines such as the Ayurveda, Unani and Siddha. With the passage of time there is increase in demand of this plant, so it is uprooted in its earlier stage and plant is becoming endangered. There are some biotechnological methods like in vitro propagation and in vitro conservation which can protect the medicinal plants to be extinct. These two methods help to produce maximum plants in less time and conserved the plant for long time. These are also very useful in storing valuable germplasms. This review is mainly focused on in vitro propagation and conservation of the Swertia chirayita.

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“…Se ha utilizado la encapsulación en plantas como Centella asiatica (L.) Urban (Prasad et al, 2014), en análisis por cromatografía líquida de alta eficiencia (HPLC), las plantas obtenidas de semillas sintética expresaron uniformidad cuantitativa y cualitativa de los compuestos de interés como los centellósidos. Los agentes encapsulantes más utilizados en la elaboración de semillas sintéticas son el alginato de sodio en complejo con cloruro de calcio dihidratado, ambos en diferentes concen-traciones y tiempos de polimerización (Standardi y Micheli, 2012;Sharma et al, 2013), dependiendo de la especie con que se trabaje (Gantait et al, 2015). La elaboración de semillas sintéticas ha permitido la propagación y preservación a largo plazo de embriones somáticos de orquídeas (Teixeira da Silva et al, 2014) y agaves (Chandrasekhara et al, 2012).…”

Section: Introductionunclassified

Optimización de las condiciones de producción de cápsulas con microestacas para aumentar el tiempo de almacenamiento de la planta medicinal mexicana Castilleja tenuiflora (Orobanchaceae)

et al. 2019

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Antecedentes y Objetivos: Castilleja tenuiflora es una de las plantas mexicanas, silvestres y medicinales cultivadas in vitro que presentan actividad biológica antiinflamatoria y antiulcerogénica. La encapsulación de microestacas es una opción para propagar y prolongar el almacenamiento de esta planta. El objetivo de este estudio fue establecer un método para la encapsulación y conservación de microestacas de C. tenuiflora sin afectar el contenido de sus compuestos.Métodos: Las cápsulas fueron elaboradas con alginato de sodio en complejo con cloruro de calcio dihidratado como matriz y microestacas de tejido proveniente de plántulas cultivadas in vitro. Se probaron diferentes concentraciones y tiempos de polimerización, así como diferentes tiempos de almacenamiento y temperatura, para posteriormente analizar los metabolitos mayoritarios de los extractos metanólicos mediante cromatografía (HPLC) de las plantas resultantes.Resultados clave: La combinación para la producción de cápsulas con microestacas es alginato de sodio al 3% y cloruro de calcio dihidratado al 50 mM, con 25 minutos de polimerización. Las plántulas derivadas de semillas sintéticas, almacenadas por 100 días presentaron los mismos compuestos que las plantas donantes.Conclusiones: La encapsulación de microestacas de C. tenuiflora con alginato de sodio y cloruro de calcio dihidratado permite el almacenamiento del material vegetal y se mantienen sus compuestos principales, por lo que es una alternativa para mantener el genotipo de plantas elite y contrarrestar el saqueo de plantas silvestres.

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Synthetic seed production of medicinal plants: a review on influence of explants, encapsulation agent and matrix

Cited by 113 publications

References 87 publications

Tissue Culture of Corymbia and Eucalyptus

Tissue Culture of Corymbia and Eucalyptus

A mini review on in vitro propagation of Swertia chirayita an endangered medicinal plant

Optimización de las condiciones de producción de cápsulas con microestacas para aumentar el tiempo de almacenamiento de la planta medicinal mexicana Castilleja tenuiflora (Orobanchaceae)

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