2011
DOI: 10.5897/ajb11.492
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Synthetic seed technology for encapsulation and regrowth of in vitro-derived Acacia hyrid shoot and axillary buds

Abstract: In this study, various concentrations of sodium alginate solutions and calcium chloride solutions were tested in order to optimize the size, shape and texture of alginate synthetic seeds or beads for Acacia hybrid bud-sprouting. The shoot buds and axillary buds from in vitro Acacia hybrids, as explants were encapsulated with 2 to 5% sodium alginate (w/v) in the Murashige and Skoog (MS) free of calcium salt solution solvent and exposed to 25 to 100 mM calcium chloride solution (CaCl 2 .2H 2 O). Rounded beads we… Show more

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Cited by 28 publications
(4 citation statements)
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“…Synthetic seeds of S. vomeracea ( Figure 3B) were produced by encapsulating PLBs with 3% sodium alginate and solidification in 75 mM of CaCl 2 . According to the previous reports, amounts of sodium alginate and CaCl 2 used for preparation of the encapsulation matrix are 3%-4% and 75-100 mm, respectively (Daud et al, 2008;Geetha et al, 2009;Sarmah et al, 2010;Nor Asmah et al, 2011). The encapsulation matrix prepared by the aforesaid procedure provides earlier germination of synthetic seeds with higher rates (Saiprasad and Polisetty, 2003).…”
Section: Synthetic Seed Production and Acclimatizationmentioning
confidence: 99%
See 1 more Smart Citation
“…Synthetic seeds of S. vomeracea ( Figure 3B) were produced by encapsulating PLBs with 3% sodium alginate and solidification in 75 mM of CaCl 2 . According to the previous reports, amounts of sodium alginate and CaCl 2 used for preparation of the encapsulation matrix are 3%-4% and 75-100 mm, respectively (Daud et al, 2008;Geetha et al, 2009;Sarmah et al, 2010;Nor Asmah et al, 2011). The encapsulation matrix prepared by the aforesaid procedure provides earlier germination of synthetic seeds with higher rates (Saiprasad and Polisetty, 2003).…”
Section: Synthetic Seed Production and Acclimatizationmentioning
confidence: 99%
“…Sodium alginate (3%) solution was prepared for encapsulation of the protocorm-like bodies and CaCl 2 (75 mM) was prepared for solidification of the synthetic encapsulation matrix, respectively (Aquea et al, 2008;Daud et al, 2008;Geetha et al, 2009;Sarmah et al, 2010;Nor Asmah et al, 2011). The chemical content of Orchimax medium without activated charcoal was used as the synthetic culture medium (artificial endosperm).…”
Section: Preparation Of Coating Matrix and Encapsulation Of The Plbmentioning
confidence: 99%
“…Em um trabalho realizado com embriões somáticos de Piper hispidinervium, conhecida como pimenta longa, constatou que o acréscimo de meio de cultura MS no encapsulamento, possibilitou significativamente o aumento da conversão desses embriões em plântulas, quando comparado com a não utilização do meio (PEREIRA, 2008). Em outro estudo, realizado com Acacia hybrid, mostrou que independente da concentração de alginato de sódio e de CaCl2, ocorreu alta taxa germinativa (ASMAH et al, 2011).…”
Section: Discussionunclassified
“…Las observaciones se realizaron a los 9, 12, 15, 18 y 21 días después de la siembra. El criterio para definir la germinación de la semilla sintética se definió cuando el brote rompió la cápsula (Asmah et al, 2011). La viabilidad fue considerada cuando los explantes encapsulados presentaron una apariencia verde, sin amarillamiento y sin partes necrosadas (Micheli et al, 2007).…”
Section: Efecto De Las Concentraciones De Los Agentes Encapsulantes Yunclassified