Synthetic Toll-like receptor agonists for the development of powerful malaria vaccines: a patent review

Kaur, Arvinder; Kannan, Deepika; Mehta, S.K.; Singh, Shailja

doi:10.1080/13543776.2018.1530217

Cited by 14 publications

(14 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It also seems reasonable to assume that TLR6 becomes activated during infection by the V. alginolyticus △ vscC strain. In mammalian cells, since activation of TLRs serves to promote innate responses and initiate adaptive immunity ( 82 , 83 ), such features are of interest because these pathways can be exploited as potential vaccine adjuvants ( 84 , 85 ). TLR6 is located on the plasma membranes, recognizing extracellular microbial pathogenic molecules with distinct PAMPs and inducing inflammation through MyD88- and TRAF6-mediated activation of NF-κB in human ( 86 ).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Virulence of Vibrio alginolyticus Accentuates Apoptosis and Immune Rigor in the Oyster Crassostrea hongkongensis

et al. 2021

View full text Add to dashboard Cite

Vibrio species are ubiquitously distributed in marine environments, with important implications for emerging infectious diseases. However, relatively little is known about defensive strategies deployed by hosts against Vibrio pathogens of distinct virulence traits. Being an ecologically relevant host, the oyster Crassostrea hongkongensis can serve as an excellent model for elucidating mechanisms underlying host-Vibrio interactions. We generated a Vibrio alginolyticus mutant strain (V. alginolyticus△vscC) with attenuated virulence by knocking out the vscC encoding gene, a core component of type III secretion system (T3SS), which led to starkly reduced apoptotic rates in hemocyte hosts compared to the V. alginolyticusWT control. In comparative proteomics, it was revealed that distinct immune responses arose upon encounter with V. alginolyticus strains of different virulence. Quite strikingly, the peroxisomal and apoptotic pathways are activated by V. alginolyticusWT infection, whereas phagocytosis and cell adhesion were enhanced in V. alginolyticus△vscC infection. Results for functional studies further show that V. alginolyticusWT strain stimulated respiratory bursts to produce excess superoxide (O2•−) and hydrogen peroxide (H2O2) in oysters, which induced apoptosis regulated by p53 target protein (p53tp). Simultaneously, a drop in sGC content balanced off cGMP accumulation in hemocytes and repressed the occurrence of apoptosis to a certain extent during V. alginolyticus△vscC infection. We have thus provided the first direct evidence for a mechanistic link between virulence of Vibrio spp. and its immunomodulation effects on apoptosis in the oyster. Collectively, we conclude that adaptive responses in host defenses are partially determined by pathogen virulence, in order to safeguard efficiency and timeliness in bacterial clearance.

show abstract

Section: Discussionmentioning

confidence: 99%

“…immunity (82,83), such features are of interest because these pathways can be exploited as potential vaccine adjuvants (84,85). TLR6 is located on the plasma membranes, recognizing extracellular microbial pathogenic molecules with distinct PAMPs and inducing inflammation through MyD88-and TRAF6-mediated activation of NF-kB in human (86).…”

Section: Discussionmentioning

confidence: 99%

Virulence of Vibrio alginolyticus Accentuates Apoptosis and Immune Rigor in the Oyster Crassostrea hongkongensis

et al. 2021

View full text Add to dashboard Cite

show abstract

“…In a major, highly recommended review article, Irvine and coworkers considered that biotechnology combined with the nanomaterials science could bring about safer and more efficient vaccine formulations with major roles for the nanoparticles (NPs) [ 116 ]. In a fast moving field encompassing cancer nanotechnology [ 50 , 89 , 90 , 95 , 117 ], vaccinology against pathogenic viruses [ 61 , 62 , 96 , 97 , 98 , 99 , 100 , 106 , 118 ], and microbes such as bacteria [ 19 , 108 , 112 , 119 , 120 ], fungi [ 119 ], and parasites [ 22 , 120 , 121 , 122 , 123 , 124 ], elegant nanomaterials that are difficult to scale up for commercialization cannot become useful in clinics [ 116 , 125 ].…”

Section: Nanoparticlesmentioning

confidence: 99%

“…Against malaria, adjuvants have to induce both antibodies and helper T cells; non-inflammatory polystyrene nanoparticles (PS NPs) as adjuvants and a protein conserved across several Plasmodium species named MSP4/5 as antigen, Th1 and Th2 immune responses was obtained; there was 50–80% protection against blood-stage malaria linked to interferon–gamma production [ 120 ]. Currently, no efficient vaccine against malaria in clinics is available; efforts to develop malaria vaccines have been reviewed [ 121 , 122 , 123 ]. Both discovery of better immunostimulatory formulations and adjuvants are needed.…”

Section: Nanoparticlesmentioning

confidence: 99%

Supramolecular Nanostructures for Vaccines

Carmona-Ribeiro

2021

Biomimetics

View full text Add to dashboard Cite

Although this is an era of pandemics and many devastating diseases, this is also a time when bionanotechnology flourishes, illuminating a multidisciplinary field where vaccines are quickly becoming a balsam and a prevention against insidious plagues. In this work, we tried to gain and also give a deeper understanding on nanovaccines and their way of acting to prevent or cure cancer, infectious diseases, and diseases caused by parasites. Major nanoadjuvants and nanovaccines are temptatively exemplified trying to contextualize our own work and its relative importance to the field. The main properties for novel adjuvants seem to be the nanosize, the cationic character, and the biocompatibility, even if it is achieved in a low dose-dependent manner.

show abstract

“…Flagellin ( iC) inherently attaches to toll-like receptor 5 (TLR5) as a natural agonist. FliC protein is a highly conserved bacterial protein; it can stimulate the humoral and cellular immune system by producing antibodies, cytokines, and interleukins that prevent the parasite entry in the pre-erythrocytic stage of malaria [23][24][25]. Therefore, the present study aimed to design a multi-epitope vaccine by utilizing bioinformatics methods based on PfGARP epitopes to elicit a humoral immune response against blood-stage of malaria infection.…”

Section: Introductionmentioning

confidence: 99%

A Multi-Epitope Vaccine Designed Against Blood-Stage of Malaria: An Immunoinformatic And Structural Approach

Atapour

Vosough

Jafari

et al. 2021

Preprint

View full text Add to dashboard Cite

Malaria is a complex disease caused by genus Plasmodiumis parasites and is the leading cause of morbidity and mortality worldwide. The most severe form of malaria disease is caused by Plasmodium falciparum. A combination of different approaches is needed to control malaria, and on the other hand, resistance to first-line drugs and insecticides makes the need for an effective vaccine more mandatory than ever. Erythrocyte parasites have the most clinical symptoms, so designing the potential vaccine for this stage of infection could be very helpful. In this research, we used various bioinformatics tools to design an effective antibody-inducing multi-epitope vaccine against the blood-stage of malaria infection. For this purpose, we selected the malaria PfGARP protein as the target here. The predicted B and HTL epitopes and flagellin molecule (as an adjuvant) were connected with suitable linkers and the final construct vaccine was designed. The various properties of this construct, including physicochemical properties, 3D structures, molecular docking, molecular simulations, and in silico cloning were then carried out. Based on preliminary findings, our designed fusion construct could be proposed as a novel potential vaccine candidate against Malaria. However, in vitro and in vivo studies are essential for further validation.

show abstract

Synthetic Toll-like receptor agonists for the development of powerful malaria vaccines: a patent review

Cited by 14 publications

References 37 publications

Virulence of Vibrio alginolyticus Accentuates Apoptosis and Immune Rigor in the Oyster Crassostrea hongkongensis

Virulence of Vibrio alginolyticus Accentuates Apoptosis and Immune Rigor in the Oyster Crassostrea hongkongensis

Supramolecular Nanostructures for Vaccines

A Multi-Epitope Vaccine Designed Against Blood-Stage of Malaria: An Immunoinformatic And Structural Approach

Contact Info

Product

Resources

About