Synthetic vaccine (SBm7462) against the cattle tick Rhipicephalus (Boophilus) microplus: Preservation of immunogenic determinants in different strains from South America

Peconick, Ana Paula; Sossai, Sidimar; Girão, Flávia A; Rodrigues, Marina Quadrio Rapouso Branco; Silva, C.H. Souza e; Patarroyo, Adriana; Vargas, Marlene; Patarroyo, J. H.

doi:10.1016/j.exppara.2007.12.007

Cited by 31 publications

(22 citation statements)

References 22 publications

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“…Antibody titers have been shown to correlate with protection, however, the epitopes found in Bm86 that induce protective immune responses in cattle are not extensively characterized. Linear B-cell epitopes previously identified by Odongo et al [15] and three synthetic peptides shown to elicit protective antibody responses characterized by Patarroyo et al [16] and Peconick et al [17] are indicated in Figure 2. When compared to Yeerongpilly and Camcord, there was only one amino acid difference within the linear B-cell epitopes present in South Texas isolates, a lysine (L) in place of glutamic acid (E) at position 560.…”

Section: Resultsmentioning

confidence: 99%

“…When compared to Yeerongpilly and Camcord, there was only one amino acid difference within the linear B-cell epitopes present in South Texas isolates, a lysine (L) in place of glutamic acid (E) at position 560. Peconick et al [17] evaluated three immunogenic epitopes in the synthetic vaccine SBm7462 based on the Australian Yeerongpilly strain. There were 4 total amino acid differences observed within these three previously described peptides shown to induce protective immune responses in cattle.…”

Section: Resultsmentioning

confidence: 99%

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Bm86 midgut protein sequence variation in South Texas cattle fever ticks

et al. 2010

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BackgroundCattle fever ticks, Rhipicephalus (Boophilus) microplus and R. (B.) annulatus, vector bovine and equine babesiosis, and have significantly expanded beyond the permanent quarantine zone established in South Texas. Currently, there are no vaccines approved for use within the United States for controlling these vectors. Vaccines developed in Australia and Cuba based on the midgut antigen Bm86 have variable efficacy against cattle fever ticks. A possible explanation for this variation in vaccine efficacy is amino acid sequence divergence between the recombinant Bm86 vaccine component and native Bm86 expressed in ticks from different geographical regions of the world.ResultsThere was 91.8% amino acid sequence identity in Bm86 among R. microplus and R. annulatus sequenced from South Texas infestations. When South Texas isolates were compared to the Australian Yeerongpilly and Cuban Camcord vaccine strains, there was 89.8% and 90.0% identity, respectively. Most of the sequence divergence was focused in one region of the protein, amino acids 206-298. Hydrophilicity profiles revealed that two short regions of Bm86 (amino acids 206-210 and 560-570) appear to be more hydrophilic in South Texas isolates compared to vaccine strains. Only one amino acid difference was found between South Texas and vaccine strains within two previously described B-cell epitopes. A total of 4 amino acid differences were observed within three peptides previously shown to induce protective immune responses in cattle.ConclusionsSequence differences between South Texas isolates and Yeerongpilly and Camcord strains are spread throughout the entire Bm86 sequence, suggesting that geographic variation does exist. Differences within previously described B-cell epitopes between South Texas isolates and vaccine strains are minimal; however, short regions of hydrophilic amino acids found unique to South Texas isolates suggest that additional unique surface exposed peptides could be targeted.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Bm86 midgut protein sequence variation in South Texas cattle fever ticks

et al. 2010

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“…The analyses indicated that the SBm7462 gene is conserved in South American tick populations. The SBm7462 conservation reported supports the use of this peptide as antigen in a vaccine against different tick populations (PECONICK et al, 2008).…”

Section: Anti-tick Vaccines Currently Availablementioning

confidence: 88%

New approaches toward anti-Rhipicephalus (Boophilus) microplus tick vaccine

Parizi¹,

Pohl²,

Masuda³

et al. 2009

RBPV

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The tick Rhipicephalus (Boophilus) microplus (formerly Boophilus microplus) is the major ectoparasite affecting livestock in America, Asia, Africa, and Oceania. Conventional tick control is based on the use of acaricides but immunization of bovines with tick gut proteins induces only a partial protective immune response. Based on this information, distinct research groups have explored the possibility of protecting the animals by inducing an immune response against other tick proteins. However, the antigens so far described do not induce the necessary protection for suppressing the use of acaricides. Currently, several groups are engaged in identifying new tick proteins to be used as targets for the development of new vaccines. This approach focuses on the enhancement of the immunogenicity of antigens already tested by incorporating new adjuvants or formulations and by searching for new antigens. This paper reviews the work done by Brazilian researchers to develop a vaccine against this tick.Keywords: Boophilus microplus, Rhipicephalus microplus, vaccine, tick, control. ResumoO carrapato Rhipicephalus (Boophilus) microplus (anteriormente Boophilus microplus) é o principal ectoparasita que afeta bovinos na América, Ásia, África e Oceania e o seu controle é tradicionalmente realizado através do uso de acaricidas. Experimentos de imunização com proteínas do carrapato mostram que a resposta imune desenvolvida pelos bovinos vacinados protege, em parte, os animais do parasitismo. Baseado nessas observações, vários grupos de pesquisa exploram a possibilidade de proteger os animais pela indução de uma resposta imune contra proteínas do carrapato. Entretanto, os antígenos já caracterizados não asseguram o grau de proteção necessário para suprimir o uso de acaricidas. Portanto, esses grupos de pesquisa estão engajados na tentativa de identificar novas proteínas que possam ser utilizadas para o desenvolvimento de novas vacinas, as quais possam induzir maior imunogenicidade de que os antígenos já testados, através do uso de novas formulações e/ou pela incorporação de adjuvantes. O presente artigo apresenta uma revisão da literatura sobre os resultados obtidos por pesquisadores brasileiros no desenvolvimento de vacinas contra o carrapato.

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“…Vaccines such as Bm86 and Bm95 are available in the market for the partial control of B. microplus; however, producing more effective vaccines needs exploration of new antigens and making novel recombinants [3]. Using Bm86 protein peptides' parts as a peptidal vaccine has been studied [4,5].…”

Section: Introductionmentioning

confidence: 99%

Designing and Modeling of Complex DNA Vaccine Based on Tropomyosin Protein of Boophilus Genus Tick

Ranjbar

Gupta

Ghorban

et al. 2014

Appl Biochem Biotechnol

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Boophilus tick is a bloodsucking ectoparasite that transfers some pathogens, reducing production and thus leading to economical losses in the cattle industry. Tropomyosin (TPM) protein is a salivary protein, has actin regulator activity, and plays an important role in immune reactions against parasites. In the current study, besides developing a safe, effective, and broad spectrum protective measure against Boophilus genus tick based on TPM protein, we attempted to minimize possible problems occurring in the design of polytopic vaccines. Briefly, the steps that were followed in the present study were as follows: retrieving sequences and finding the mutational/conservative regions, selecting consensus and high immunogenic epitopes of B and CD4(+) T cells by different approaches, three-dimensional structure (3D structure) prediction and representation of epitopes and highly variable/conserve regions, designing vaccinal construct by fusion of B and T cell epitopes by special patterns and improving immunogenicity, evaluation of the constructs' primary structure and posttranslational modification, calculation of hydrophobic regions, reverse translation, codon optimization, open reading frame checking, insertion of start/end codon, Kozak sequence, and finally constructing the DNA vaccine. Variation plot showed some shared epitopes among the ticks' and mites' species that some might be effective only in some species. Finally, by following the steps mentioned above, two constructs for B and T cells were achieved. Checking constructs revealed their reliability and efficacy for in vitro production and utilization. Successful in silico modeling is an essential step of designing vigorous vaccines. We developed a novel protective and therapeutic vaccine against Boophilus genus (based on TPM protein). At the next step, constructed DNA vaccine would be produced in vitro and administrated to cattle, and its potency to induction of immune response and protection against Boophilus genus as well as other ticks and mites will be evaluated.

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Synthetic vaccine (SBm7462) against the cattle tick Rhipicephalus (Boophilus) microplus: Preservation of immunogenic determinants in different strains from South America

Cited by 31 publications

References 22 publications

Bm86 midgut protein sequence variation in South Texas cattle fever ticks

Bm86 midgut protein sequence variation in South Texas cattle fever ticks

New approaches toward anti-Rhipicephalus (Boophilus) microplus tick vaccine

Designing and Modeling of Complex DNA Vaccine Based on Tropomyosin Protein of Boophilus Genus Tick

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