Synthetic Viruslike Particles and Hybrid Constructs Based on Lipopeptide Self‐Assembly

Perriman, Adam W.; Williams, David; Jackson, Andrew; Grillo, Isabelle; Koomullil, Jimy; Ghasparian, Arin; Mann, Stephen

doi:10.1002/smll.200901186

Cited by 17 publications

(14 citation statements)

References 19 publications

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“…The MSN-PEI/siRNAs coated with the 10 kDa and 25 kDa PEI were capable of knocking down of GFP level by 55% and 60%, respectively, in GFP expressing HEPA-1 cells. Use of low molecular weight PEI (1.2 kDa) in PEI-MSN formulation can silence both exogenous and endogenous genes with reduced cytotoxicity (Perriman et al, 2010). Favorable binding of siRNA at the surface and facilitated encapsulation of small drugs within the porous channels having high loading capacity make MSN very useful carrier particularly for drug/ siRNA combination delivery.…”

Section: Mesoporous Silica Nanoparticlesmentioning

confidence: 99%

Polymers in Small-Interfering RNA Delivery

Singha

Namgung

Kim

2011

Nucleic Acid Therapeutics

185

112

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This review will cover the current strategies that are being adopted to efficiently deliver small interfering RNA using nonviral vectors, including the use of polymers such as polyethylenimine, poly(lactic-co-glycolic acid), polypeptides, chitosan, cyclodextrin, dendrimers, and polymers-containing different nanoparticles. The article will provide a brief and concise account of underlying principle of these polymeric vectors and their structural and functional modifications which were intended to serve different purposes to affect efficient therapeutic outcome of small-interfering RNA delivery. The modifications of these polymeric vectors will be discussed with reference to stimuli-responsiveness, target specific delivery, and incorporation of nanoconstructs such as carbon nanotubes, gold nanoparticles, and silica nanoparticles. The emergence of small-interfering RNA as the potential therapeutic agent and its mode of action will also be mentioned in a nutshell.

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Section: Mesoporous Silica Nanoparticlesmentioning

confidence: 99%

Polymers in Small-Interfering RNA Delivery

Singha

Namgung

Kim

2011

Nucleic Acid Therapeutics

185

112

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“…These are complex antigenic structures with an already proven capacity for efficient induction of IgG responses; importantly, this is achieved without adjuvant requirement in rabbits [17] and pigs (R.S, unpublished data). SVLP are formed by lipopeptide self-assembly into spherical nanostructures in the 20–30 nm size range, which resemble small virus-like particles in their shape, size and chemical composition [18], [19]. Like naturally-derived VLPs, SVLP can be exploited for multivalent presentation of antigens across their surface (Fig.…”

Section: Introductionmentioning

confidence: 99%

Synthetic Virus-Like Particles Target Dendritic Cell Lipid Rafts for Rapid Endocytosis Primarily but Not Exclusively by Macropinocytosis

Sharma¹,

Ghasparian

Robinson

et al. 2012

PLoS ONE

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DC employ several endocytic routes for processing antigens, driving forward adaptive immunity. Recent advances in synthetic biology have created small (20–30 nm) virus-like particles based on lipopeptides containing a virus-derived coiled coil sequence coupled to synthetic B- and T-cell epitope mimetics. These self-assembling SVLP efficiently induce adaptive immunity without requirement for adjuvant. We hypothesized that the characteristics of DC interaction with SVLP would elaborate on the roles of cell membrane and intracellular compartments in the handling of a virus-like entity known for its efficacy as a vaccine. DC rapidly bind SVLP within min, co-localised with CTB and CD9, but not caveolin-1. In contrast, internalisation is a relatively slow process, delivering SVLP into the cell periphery where they are maintained for a number of hrs in association with microtubules. Although there is early association with clathrin, this is no longer seen after 10 min. Association with EEA-1+ early endosomes is also early, but proteolytic processing appears slow, the SVLP-vesicles remaining peripheral. Association with transferrin occurs rarely, and only in the periphery, possibly signifying translocation of some SVLP for delivery to B-lymphocytes. Most SVLP co-localise with high molecular weight dextran. Uptake of both is impaired with mature DC, but there remains a residual uptake of SVLP. These results imply that DC use multiple endocytic routes for SVLP uptake, dominated by caveolin-independent, lipid raft-mediated macropinocytosis. With most SVLP-containing vesicles being retained in the periphery, not always interacting with early endosomes, this relates to slow proteolytic degradation and antigen retention by DC. The present characterization allows for a definition of how DC handle virus-like particles showing efficacious immunogenicity, elements valuable for novel vaccine design in the future.

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“…With multiple copies of desired antigens attached to the C terminus of the lipopeptide building blocks, the SVLPs not only retained advantages of naturally derived VLPs as antigen delivery vehicles, but also gained the possibility to be optimized using a myriad of synthetic chemical methods. The method was expanded further into a common one85 to form hollow spheres with calcium phosphate, and to explore different applications to generate immune response 86…”

Section: Artificial Basic Building Blocks For Lifementioning

confidence: 99%

Progressive Macromolecular Self‐Assembly: From Biomimetic Chemistry to Bio‐Inspired Materials

et al. 2013

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Macromolecular self-assembly (MSA) has been an active and fruitful research field since the 1980s, especially in this new century, which is promoted by the remarkable developments in controlled radical polymerization in polymer chemistry, etc. and driven by the demands in bio-related investigations and applications. In this review, we try to summarize the trends and recent progress in MSA in relation to biomimetic chemistry and bio-inspired materials. Our paper covers representative achievements in the fabrication of artificial building blocks for life, cell-inspired biomimetic materials, and macromolecular assemblies mimicking the functions of natural materials and their applications. It is true that the current status of the deliberately designed and obtained nano-objects based on MSA including a variety of micelles, multicompartment vesicles, and some hybrid and complex nano-objects is at their very first stage to mimic nature, but significant and encouraging progress has been made in achieving a certain similarity in morphologies or properties to that of natural ones. Such achievements also demonstrate that MSA has played an important and irreplaceable role in the grand and long-standing research of biomimetic and bio-inspired materials, the future success of which depends on mutual and persistent efforts in polymer science, material science, supramolecular chemistry, and biology.

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Synthetic Viruslike Particles and Hybrid Constructs Based on Lipopeptide Self‐Assembly

Cited by 17 publications

References 19 publications

Polymers in Small-Interfering RNA Delivery

Polymers in Small-Interfering RNA Delivery

Synthetic Virus-Like Particles Target Dendritic Cell Lipid Rafts for Rapid Endocytosis Primarily but Not Exclusively by Macropinocytosis

Progressive Macromolecular Self‐Assembly: From Biomimetic Chemistry to Bio‐Inspired Materials

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