Synthetically Programmable Nanoparticle Superlattices Using a Hollow Three-Dimensional Spacer Approach*

Auyeung, Evelyn; Cutler, Joshua I.; Macfarlane, Robert J.; Jones, Matthew R.; Wu, Jinsong; Liu, George; Zhang, Ke; Osberg, Kyle D.; Mirkina, Chad A.

doi:10.4324/9780429200151-29

“…SNAP strategy offers several unique advantages. First, homoand hetero-neutralizing aptamers can be easily anchored with high density and homogeneous orientation on the same gold-nanoparticle 15,16 , affording improved binding affinity and simultaneous blocking of different RBDs on the trimeric-spike. Second, the particle scaffold can provide a dimensionally matched interaction between spherical neutralizing aptamers (∼5 nm) and the trimeric-spike (∼5 nm).…”

Section: Mainmentioning

confidence: 99%

Spherical Neutralizing Aptamer Inhibits SARS-CoV-2 Infection

Sun¹,

Liu²,

Song³

et al. 2021

Preprint

0

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New neutralizing agents against SARS-CoV-2 and the associated mutant strains are urgently needed for the treatment and prophylaxis of COVID-19. Herein, we develop a spherical cocktail neutralizing aptamer-gold nanoparticle (SNAP) to synergistically block the interaction of SARS-CoV-2 receptor-binding domain (RBD) and angiotensin-converting enzyme-2 (ACE2). Taking advantage of the simultaneous recognition of multi-homologous and multi-heterogenous neutralizing aptamers and dimensionally matched nano-scaffolds, the SNAP exhibits increased affinity to the RBD with a dissociation constant value of 5.46 pM and potent neutralization against authentic SARS-CoV-2 with a half-maximal inhibitory concentration of 142.80 aM. Additional benefits include the multi-epitope blocking capability of the aptamer cocktail and the steric hindrance of the nano-scaffold, which further covers the ACE2 binding interfaces and affects the conformational transition of the spike protein. As a result, the SNAP strategy exhibits broad neutralizing activity, almost completely blocking the infection of<a> N501Y</a> and D614G mutant strains. Overall, the SNAP strategy provides a new direction for development of anti-virus infection mechanisms, both to fight the COVID-19 pandemic and serve as a powerful technical reserve for future unknown pandemics.

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“…8c) [80]. Moreover, not only were various self-assembled structures from a single inorganic nanoparticle to a superlattice achieved [81][82][83][84][85][86][87], the properties of the assembled structures, such as temperature-dependent crystal topology [88], crystal surface energy [89], and dynamic DNA strand substitution [90], were also studied in detail. The mechanism of DNA-templated nanoparticle self-assembly was explained by the repulsive force between colloids [91] and the enthalpy change [92].…”

Section: Dna-programmed Nanoparticle Super-lattice Crystallizationmentioning

confidence: 99%

DNA-Programmed Chemical Synthesis of Polymers and Inorganic Nanomaterials

Xu

¹

,

Winterwerber

²

,

Ng

³

et al. 2020

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DNA nanotechnology, based on sequence-specific DNA recognition, could allow programmed self-assembly of sophisticated nanostructures with molecular precision. Extension of this technique to the preparation of broader types of nanomaterials would significantly improve nanofabrication technique to lower nanometer scale and even achieve single molecule operation. Using such exquisite DNA nanostructures as templates, chemical synthesis of polymer and inorganic nanomaterials could also be programmed with unprecedented accuracy and flexibility. This review summarizes recent advances in the synthesis and assembly of polymer and inorganic nanomaterials using DNA nanostructures as templates, and discusses the current challenges and future outlook of DNA templated nanotechnology.

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“…8c) [80]. Moreover, not only were various self-assembled structures from a single inorganic nanoparticle to a superlattice achieved [81][82][83][84][85][86][87], the properties of the assembled structures, such as temperature-dependent crystal topology [88], crystal surface energy [89], and dynamic DNA strand substitution [90], were also studied in detail. The mechanism of DNA-templated nanoparticle self-assembly was explained by the repulsive force between colloids [91] and the enthalpy change [92].…”

Section: Dna-programmed Nanoparticle Super-lattice Crystallizationmentioning

confidence: 99%

DNA-Programmed Chemical Synthesis of Polymers and Inorganic Nanomaterials

Xu

¹

,

Winterwerber

²

,

Ng

³

et al. 2020

View full text Add to dashboard Cite

DNA nanotechnology, based on sequence-specific DNA recognition, could allow programmed self-assembly of sophisticated nanostructures with molecular precision. Extension of this technique to the preparation of broader types of nanomaterials would significantly improve nanofabrication technique to lower nanometer scale and even achieve single molecule operation. Using such exquisite DNA nanostructures as templates, chemical synthesis of polymer and inorganic nanomaterials could also be programmed with unprecedented accuracy and flexibility. This review summarizes recent advances in the synthesis and assembly of polymer and inorganic nanomaterials using DNA nanostructures as templates, and discusses the current challenges and future outlook of DNA templated nanotechnology.

show abstract

Synthetically Programmable Nanoparticle Superlattices Using a Hollow Three-Dimensional Spacer Approach*

Cited by 11 publications

References 1 publication

Spherical Neutralizing Aptamer Inhibits SARS-CoV-2 Infection

Spherical Neutralizing Aptamer Inhibits SARS-CoV-2 Infection

DNA-Programmed Chemical Synthesis of Polymers and Inorganic Nanomaterials

DNA-Programmed Chemical Synthesis of Polymers and Inorganic Nanomaterials

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