Synthetische Adhäsion von Integrin‐Liposomen als minimales Zellmodell

Frohnmayer, Johannes Patrick; Brüggemann, Dorothea; Eberhard, Christian; Neubauer, Stefanie; Mollenhauer, Christine; Boehm, Heike; Kessler, Horst; Geiger, Benjamin; Spatz, Joachim P.

doi:10.1002/ange.201503184

Cited by 3 publications

(1 citation statement)

References 51 publications

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“…The dissipation changes indicate that the proteoliposomes maintain an intact vesicle shape. Frohnmayer et al (2015a , b ) demonstrated that the adhesion of similarly prepared proteoliposomes on fibrinogen-coated surfaces, a protein which contains the RGD motif, results in decreased frequency and increased dissipation values within the same order of magnitude monitored in this study.…”

Section: Discussionsupporting

confidence: 72%

Tuning RGD Motif and Hyaluronan Density to Study Integrin Binding

2018

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Well-controlled surfaces with immobilized substrates enable novel approaches to investigate specific aspects of biological processes related to cell adhesion or motility. A subset of integrins, cellular transmembrane glycoproteins, recognize the evolutionarily conserved tripeptide sequence RGD, and anchor cells to their surrounding proteins as well as mediate bidirectional signaling. In this study, the main question was how co-presentation of hyaluronan (HA), an essential component of the extracellular matrix (ECM), and the RGD motif affect integrin binding. We report a method to prepare self-assembled monolayers on gold surfaces, co-presenting the cell adhesive RGD motif and small HA molecules, to investigate integrin containing proteoliposome binding. This technique enables an independent adjustment of the RGD motif and HA density while maintaining a passivating background: Layer formation and subsequent interactions with αIIbβ3 integrins, which are reconstituted in liposomes, was monitored by label-free quartz crystal microbalance with dissipation monitoring (QCM-D). Exceeding a critical RGD motif density of 40% results in enhanced binding of proteoliposomes. Co-presentation studies with varying HA and constant RGD motif density demonstrate that marginal amounts of HA are sufficient to prevent integrin binding. These findings are of specific importance in relation to cancer cell microenvironments, which show highly enriched HA in the surrounding ECM to reduce adhesion properties.

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Section: Discussionsupporting

confidence: 72%

Tuning RGD Motif and Hyaluronan Density to Study Integrin Binding

2018

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Bacteria‐Based Production of Thiol‐Clickable, Genetically Encoded Lipid Nanovesicles

et al. 2019

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Despite growing researchefforts on the preparation of (bio)functional liposomes,s ynthetic capsules cannot reach the densities of protein loading and the control over peptide displaythat is achieved by natural vesicles.Herein, amicrobial platform for high-yield production of lipidic nanovesicles with clickable thiol moieties in their outer corona is reported. These nanovesicles show lows ized ispersity,a re decorated with ad ense,p erfectly oriented, and customizable corona of transmembrane polypeptides.F urthermore,t his approach enables encapsulation of soluble proteins into the nanovesicles. Due to the mild preparation and loading conditions (absence of organic solvents,p Hg radients,o rd etergents) and their straightforwardsurface functionalization, whichtakes advantage of the diversity of commercially available maleimide derivatives,b acteria-based proteoliposomes are an attractive eco-friendly alternative that can outperform currently used liposomes.

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Bacteria‐Based Production of Thiol‐Clickable, Genetically Encoded Lipid Nanovesicles

et al. 2019

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Despite growing research efforts on the preparation of (bio)functional liposomes, synthetic capsules cannot reach the densities of protein loading and the control over peptide display that is achieved by natural vesicles. Herein, a microbial platform for high‐yield production of lipidic nanovesicles with clickable thiol moieties in their outer corona is reported. These nanovesicles show low size dispersity, are decorated with a dense, perfectly oriented, and customizable corona of transmembrane polypeptides. Furthermore, this approach enables encapsulation of soluble proteins into the nanovesicles. Due to the mild preparation and loading conditions (absence of organic solvents, pH gradients, or detergents) and their straightforward surface functionalization, which takes advantage of the diversity of commercially available maleimide derivatives, bacteria‐based proteoliposomes are an attractive eco‐friendly alternative that can outperform currently used liposomes.

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Synthetische Adhäsion von Integrin‐Liposomen als minimales Zellmodell

Cited by 3 publications

References 51 publications

Tuning RGD Motif and Hyaluronan Density to Study Integrin Binding

Tuning RGD Motif and Hyaluronan Density to Study Integrin Binding

Bacteria‐Based Production of Thiol‐Clickable, Genetically Encoded Lipid Nanovesicles

Bacteria‐Based Production of Thiol‐Clickable, Genetically Encoded Lipid Nanovesicles

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