Syntrophic acetate oxidation having a key role in thermophilic phenol conversion in anaerobic membrane bioreactor under saline conditions

Rea, Victor S. Garcia; Sierra, Julian Muñoz; El‐Kalliny, Amer S.; Garcia, Daniel Cerqueda; Lindeboom, Ralph E.F.; Spanjers, Henri; Lier, Jules B. van

doi:10.1016/j.cej.2022.140305

Cited by 9 publications

(7 citation statements)

References 53 publications

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“…Then Methanothermobacter, Methanosarcina and Methanoculleus converted acetate and H 2 /CO 2 into CH 4 . Another work reported similar associations during the thermophilic conversion of phenol into CH 4 in an anaerobic membrane bioreactor [29]. There, Clostridium sensu stricto degraded phenol to acetate via benzoate, while syntrophic acetate oxidizers and Methanothermobacter associations were essential to maintain a thermodynamically favorable process.…”

Section: Reactor Microbiomes and Performances Of The Mesophilic And T...mentioning

confidence: 78%

“…Syntrophaceticus and other syntrophic bacteria oxidized the acetate from phenol into CO 2 and H 2 while Methanothermobacter produced CH 4 via hydrogenotrophic methanation. An impaired methanogenic population lead to increasing H 2 partial pressure inhibiting syntrophic acetate oxidizing bacteria and reducing the thermodynamic feasibility of phenol conversion [ 29 ]. Here, the accumulation of untreated PAC components or metabolic intermediates of PAC components degradation could have resulted in the inhibition and subsequent wash-out of hydrogenotrophic methanogens such as Methanosarcina and Methanoculleus , leading to increasing H 2 partial pressure.…”

Section: Discussionmentioning

confidence: 99%

“…To improve methane production, recent efforts have successfully employed community enrichment or biochar amendments [5,6,17,[19][20][21][22][23][24][25][26][27][28]. The taxonomic profiling of the anaerobic communities acclimatized to different PACs highlighted the relevance of syntrophic and acidogenic microorganisms during PAC components degradation [18,20,[29][30][31][32][33][34][35][36][37] and their enrichment/bioaugmentation might be a possible strategy to improve PAC degradation and methane production [2].…”

Section: Introductionmentioning

confidence: 99%

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Two-stage conversion of syngas and pyrolysis aqueous condensate into L-malate

Robazza,

Baleeiro,

Kleinsteuber

et al. 2024

Biotechnol Biofuels

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Hybrid thermochemical–biological processes have the potential to enhance the carbon and energy recovery from organic waste. This work aimed to assess the carbon and energy recovery potential of multifunctional processes to simultaneously sequestrate syngas and detoxify pyrolysis aqueous condensate (PAC) for short-chain carboxylates production. To evaluate relevant process parameters for mixed culture co-fermentation of syngas and PAC, two identical reactors were run under mesophilic (37 °C) and thermophilic (55 °C) conditions at increasing PAC loading rates. Both the mesophilic and the thermophilic process recovered at least 50% of the energy in syngas and PAC into short-chain carboxylates. During the mesophilic syngas and PAC co-fermentation, methanogenesis was completely inhibited while acetate, ethanol and butyrate were the primary metabolites. Over 90% of the amplicon sequencing variants based on 16S rRNA were assigned to Clostridium sensu stricto 12. During the thermophilic process, on the other hand, Symbiobacteriales, Syntrophaceticus, Thermoanaerobacterium, Methanothermobacter and Methanosarcina likely played crucial roles in aromatics degradation and methanogenesis, respectively, while Moorella thermoacetica and Methanothermobacter marburgensis were the predominant carboxydotrophs in the thermophilic process. High biomass concentrations were necessary to maintain stable process operations at high PAC loads. In a second-stage reactor, Aspergillus oryzae converted acetate, propionate and butyrate from the first stage into L-malate, confirming the successful detoxification of PAC below inhibitory levels. The highest L-malate yield was 0.26 ± 2.2 molL-malate/molcarboxylates recorded for effluent from the mesophilic process at a PAC load of 4% v/v. The results highlight the potential of multifunctional reactors where anaerobic mixed cultures perform simultaneously diverse process roles, such as carbon fixation, wastewater detoxification and carboxylates intermediate production. The recovered energy in the form of intermediate carboxylates allows for their use as substrates in subsequent fermentative stages.

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Section: Reactor Microbiomes and Performances Of The Mesophilic And T...mentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Two-stage conversion of syngas and pyrolysis aqueous condensate into L-malate

Robazza,

Baleeiro,

Kleinsteuber

et al. 2024

Biotechnol Biofuels

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“…The DNA extracted from all samples was submitted to the DNA Services Facility at the Roy J. Carver Biotechnology Center (UIUC) for library preparation and sequencing per their standard protocol [ 42 ]. DNA used in PCR amplification was approximately 1 ng.…”

Section: Methodsmentioning

confidence: 99%

Gut Microbiome-Host Metabolome Homeostasis upon Exposure to PFOS and GenX in Male Mice

Rashid

Dubinkina

Ahmad

et al. 2023

Toxics

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Alterations of the normal gut microbiota can cause various human health concerns. Environmental chemicals are one of the drivers of such disturbances. The aim of our study was to examine the effects of exposure to perfluoroalkyl and polyfluoroalkyl substances (PFAS)—specifically, perfluorooctane sulfonate (PFOS) and 2,3,3,3-tetrafluoro-2-(heptafluoropropoxy) propanoic acid (GenX)—on the microbiome of the small intestine and colon, as well as on liver metabolism. Male CD-1 mice were exposed to PFOS and GenX in different concentrations and compared to controls. GenX and PFOS were found to have different effects on the bacterial community in both the small intestine and colon based on 16S rRNA profiles. High GenX doses predominantly led to increases in the abundance of Clostridium sensu stricto, Alistipes, and Ruminococcus, while PFOS generally altered Lactobacillus, Limosilactobacillus, Parabacteroides, Staphylococcus, and Ligilactobacillus. These treatments were associated with alterations in several important microbial metabolic pathways in both the small intestine and colon. Untargeted LC-MS/MS metabolomic analysis of the liver, small intestine, and colon yielded a set of compounds significantly altered by PFOS and GenX. In the liver, these metabolites were associated with the important host metabolic pathways implicated in the synthesis of lipids, steroidogenesis, and in the metabolism of amino acids, nitrogen, and bile acids. Collectively, our results suggest that PFOS and GenX exposure can cause major perturbations in the gastrointestinal tract, aggravating microbiome toxicity, hepatotoxicity, and metabolic disorders.

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“…Characterizing phenol-degrading microbial communities provides an understanding of the most effective microorganisms at degrading phenol, their growth requirements and optimal conditions. This information can improve the efficiency and effectiveness of bioremediation strategies by targeting specific microorganisms or groups of microorganisms (Rea et al, 2022). Additionally, studying the microbial community's diversity can provide insight into the resilience and adaptability of the community to changing environmental conditions and perturbations.…”

Section: Functional Diversity Of Phenol-degrading Microorganismsmentioning

confidence: 99%

Bacterial degradation of phenol: a review of the current state of knowledge

Adetitun,

Tomilayo

2024

ijs

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Phenol is a toxic and recalcitrant contaminant widely used in various industries, including petrochemical, pharmaceutical, and agrochemical industries. Bacterial degradation of phenol is a promising method for treating phenol-contaminated wastewater. The biodegradation of phenol by bacteria can effectively remove it from the environment, making it a valuable alternative to traditional chemical treatment methods. This review summarizes the current knowledge on the bacterial biodegradation of phenol, including those in the petrochemical, pharmaceutical, and agrochemical sectors, the metabolic pathways involved in phenol degradation, and the factors that influence the efficiency of phenol biodegradation. This paper also discusses the challenges and limitations of using bacteria for the biodegradation of phenol, including the need for further research to improve the efficiency and sustainability of the process. The outcome of this review demonstrates that bacterial biodegradation is a promising and effective method for treating phenol-contaminated wastewater and provides a foundation for future research to improve the efficiency and continuous use of this process.

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Syntrophic acetate oxidation having a key role in thermophilic phenol conversion in anaerobic membrane bioreactor under saline conditions

Cited by 9 publications

References 53 publications

Two-stage conversion of syngas and pyrolysis aqueous condensate into L-malate

Two-stage conversion of syngas and pyrolysis aqueous condensate into L-malate

Gut Microbiome-Host Metabolome Homeostasis upon Exposure to PFOS and GenX in Male Mice

Bacterial degradation of phenol: a review of the current state of knowledge

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