Syntrophus buswellii gen. nov., sp. nov.: a Benzoate Catabolizer from Methanogenic Ecosystems

Mountfort, Douglas O.; Brulla, W. J.; Krumholz, Lee R.; Bryant, M. P.

doi:10.1099/00207713-34-2-216

Cited by 123 publications

(70 citation statements)

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“…The fat rods with a rough cell boundary usually occurred singly or in pairs. Such morphology has been previously observed for cells of anaerobic benzoate degraders (Mountfort et al, 1984) and these cells contained electron-dense regions ( Fig. 2b, arrow 4).…”

Section: Microbial Community As Revealed By Electron Microscopymentioning

confidence: 63%

“…3b). For the 10 OTUs found in the δ-Proteobacteria, two (TA7 and TA20) were closely affiliated with bacterial genera (Syntrophus and Smithella) that form syntrophic relationships with methanogens to degrade aromatic compounds such as benzoate (Mountfort et al, 1984). The other eight OTUs (66n8 % of the clones of Bacteria) appeared to form a novel cluster with no close affiliation to any known bacterial isolate.…”

Section: Microbial Community Structurementioning

confidence: 95%

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Characterization of microbial consortia in a terephthalate-degrading anaerobic granular sludge system The GenBank accession numbers for the sequences obtained in this work are AF229774–AF229793.

et al. 2001

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The microbial composition and spatial distribution in a terephthalatedegrading anaerobic granular sludge system were characterized using molecular techniques. 16S rDNA clone library and sequence analysis revealed that 785 % of 106 bacterial clones belonged to the δ subclass of the class Proteobacteria ; the remaining clones were assigned to the green non-sulfur bacteria (75 %), Synergistes (09 %) and unidentified divisions (131 %). Most of the bacterial clones in the δ-Proteobacteria formed a novel group containing no known bacterial isolates. For the domain Archaea, 817 % and 183 % of 72 archaeal clones were affiliated with Methanosaeta and Methanospirillum, respectively. Spatial localization of microbial populations inside granules was determined by transmission electron microscopy and fluorescent in situ hybridization with oligonucleotide probes targeting the novel δ-proteobacterial group, the acetoclastic Methanosaeta, and the hydrogenotrophic Methanospirillum and members of Methanobacteriaceae. The novel group included at least two different populations with identical rod-shape morphology, which made up more than 87 % of the total bacterial cells, and were closely associated with methanogenic populations to form a nonlayered granular structure. This novel group was presumed to be the primary bacterial population involved in the terephthalate degradation in the methanogenic granular consortium.

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Section: Microbial Community As Revealed By Electron Microscopymentioning

confidence: 63%

Section: Microbial Community Structurementioning

confidence: 95%

Characterization of microbial consortia in a terephthalate-degrading anaerobic granular sludge system The GenBank accession numbers for the sequences obtained in this work are AF229774–AF229793.

et al. 2001

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“…Novel Syntrophaceae member: a versatile syntrophic fatty acid degrader Our previous study (Lykidis et al, 2011) suspected that the Syntrophus-related clade (93.8% 16S rRNA similarity to Syntrophus aciditrophicus strain SB), composing 1.2% of the bacterial community and 3.1% of total metatranscriptome, may also contribute to aromatic compound degradation as characteristic of Syntrophus (Mountfort et al, 1984). Although its genome lacks the benzoate degradation pathway, we identify expression of Hdr-Ifo, ECHyd and FixABCX (Figures 2 and 3 and Supplementary Table S2), suggesting the capacity to syntrophically degrade carboxylates.…”

Section: Metatranscriptomicsmentioning

confidence: 99%

Microbial dark matter ecogenomics reveals complex synergistic networks in a methanogenic bioreactor

et al. 2015

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Ecogenomic investigation of a methanogenic bioreactor degrading terephthalate (TA) allowed elucidation of complex synergistic networks of uncultivated microorganisms, including those from candidate phyla with no cultivated representatives. Our previous metagenomic investigation proposed that Pelotomaculum and methanogens may interact with uncultivated organisms to degrade TA; however, many members of the community remained unaddressed because of past technological limitations. In further pursuit, this study employed state-of-the-art omics tools to generate draft genomes and transcriptomes for uncultivated organisms spanning 15 phyla and reports the first genomic insight into candidate phyla Atribacteria, Hydrogenedentes and Marinimicrobia in methanogenic environments. Metabolic reconstruction revealed that these organisms perform fermentative, syntrophic and acetogenic catabolism facilitated by energy conservation revolving around H 2 metabolism. Several of these organisms could degrade TA catabolism by-products (acetate, butyrate and H 2 ) and syntrophically support Pelotomaculum. Other taxa could scavenge anabolic products (protein and lipids) presumably derived from detrital biomass produced by the TA-degrading community. The protein scavengers expressed complementary metabolic pathways indicating syntrophic and fermentative step-wise protein degradation through amino acids, branched-chain fatty acids and propionate. Thus, the uncultivated organisms may interact to form an intricate syntrophy-supported food web with Pelotomaculum and methanogens to metabolize catabolic by-products and detritus, whereby facilitating holistic TA mineralization to CO 2 and CH 4 .

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“…The fermentation balances (Tables 2, 3) Three basic features render this bacterium interesting: i) The syntrophic oxidation of benzoate to acetate and carbon dioxide, ii) degradation of hydroquinone and gentisate in defined coculture, and iii) the reductive conversion of hydroquinone or gentisate to benzoate during fermentation of these substrates in pure culture. Syntrophic benzoate oxidation in defined culture has been reported for Syntrophus buswellii, an obligately syntrophic proton-reducing anaerobe which so far has not yet been grown in pure culture (Mountfort et al 1984). Until now, it is not possible to decide if our isolate is related to S. buswellii.…”

Section: Discussionmentioning

confidence: 70%

“…Two bacteria have been described degrading benzoate in syntrophic coculture with a hydrogen-consuming bacterium (Mountfort et al 1984;Tschech and Schink 1986). No fermenting bacterium degrading phendiols has been isolated in pure culture, but degradability of these compounds under methanogenic conditions has been observed in enrichment cultures and sludge samples (Szewzyk et al 1985;Young and Rivera 1985).…”

mentioning

confidence: 99%

Degradation of hydroquinone, gentisate, and benzoate by a fermenting bacterium in pure or defined mixed culture

Szewzyk

Schink

1989

Arch. Microbiol.

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Abstract. From a methanogenic fixed-bed reactor fed with hydroquinone as sole energy and carbon source, a rodshaped bacterium was isolated in pure culture which could degrade hydroquinone and gentisate (2,5-dihydroxybenzoate). In syntrophic coculture with either Desulfovibrio vulgaris or Methanospirillum hungatei, also benzoate could be degraded. Other substrates such as sugars, fatty acids, alcohols, and cyclohexane derivatives were not degraded. Sulfate, sulfite, or nitrate were not used as external electron acceptor. The isolate was a Gram-negative, non-motile, nonsporeforming strict anaerobe; the guanine-plus-cytosine content of the DNA was 53.2_+ 1.0 mol%. In pure culture, hydroquinone was degraded to acetate and benzoate, probably via an intermediate carboxylation. In syntrophic mixed cultures, all three substrates were converted completely to acetate. Phenol was never detected as a fermentation product.

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Syntrophus buswellii gen. nov., sp. nov.: a Benzoate Catabolizer from Methanogenic Ecosystems

Cited by 123 publications

References 0 publications

Characterization of microbial consortia in a terephthalate-degrading anaerobic granular sludge system The GenBank accession numbers for the sequences obtained in this work are AF229774–AF229793.

Characterization of microbial consortia in a terephthalate-degrading anaerobic granular sludge system The GenBank accession numbers for the sequences obtained in this work are AF229774–AF229793.

Microbial dark matter ecogenomics reveals complex synergistic networks in a methanogenic bioreactor

Degradation of hydroquinone, gentisate, and benzoate by a fermenting bacterium in pure or defined mixed culture

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