Syphacia muris: Response to environmental stimuli when hatching in vitro

Gulden, W.; Erp, A.J.M. van Aspert-van

doi:10.1016/0014-4894(76)90008-4

Cited by 2 publications

(1 citation statement)

References 9 publications

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“…The hatching of the eggs is dependent on the proteolytic ability of trypsin (Van der Gulden & Van Aspert-Van Erp 1976a). This has been shown by the lack of hatching after denaturation of the enzyme (Van der Gulden & Van Aspert-Van Erp 1976b). The presence of the di-sodium hydrogen orthophosphate aids the process, by producing sodium hydroxide to overcome the 'sticky layer' found externally on the (Fairbairn 1960).…”

Section: Discussionmentioning

confidence: 99%

Assessment of methods of destruction of Syphacia muris eggs

2004

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SummaryAn investigation was performed to identify methods suitable for decontamination of equipment being transferred from a facility contaminated with Syphacia muris to a new facility. Perianal samples were taken on clear sticky tape from rats known to be infected with S. muris. Tapes and attached worm eggs were treated with ethylene oxide, formaldehyde fumigation, potassium peroxysulphate, chlorine dioxide and didecyl di-methyl ammonium chloride, chlorine dioxide, alcohol=chlorhexidine, 100¯C dry heat for 30 min, ultraviolet light (UV), or left for 4 weeks at room temperature. The subsequent viability of the eggs was compared to untreated control samples. Heat and ethylene oxide produced a 100% kill rate of S. muris eggs. Formaldehyde gas and chlorine dioxide treatments showed marked effectiveness, with the number of eggs killed at 94% and 96%, respectively, while the other agents showed less ef cacy (36-78% eggs killed). The percentage of eggs not hatching in the controls was 21%. Since so few agents were effective against S. muris eggs, care should be taken when choosing a method for decontaminating a facility.

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Section: Discussionmentioning

confidence: 99%