In previous studies from this laboratory on the abrasion of dentin by brushing with dentifrice materials (1), the procedures were chosen to conform to those conditions which seemed to be present in the toothbrushing practice of the majority of dentifrice users. Toothbrushes, whole human teeth, and a crosswise brushing of the labial and lingual surfaces were employed, and under these conditions wear of exposed cementum or dentin was obtained whenever the tests were carried out with those types of dentifrices which contain abrasives.There were several details of the procedures which seemed worth further investigation. There might be some question of the influence of the brush itself in such tests, either because of its hardness or the manner in which it was used. It seemed important to ascertain the proper concentration of dentifrice to be employed in making abrasion tests, and the sites at which maximum wear takes place on the test objects. The influence of decalcification upon abrasion resistance was also studied in order to permit proper interpretation of the resultsof tests on dentifrices.The literature on abrasion testing has been reviewed in the previous paper except for the publication by Tainter and Epstein (2) which appeared recently. Their procedure makes use of human teeth and toothbrushes, but differs considerably from ours in technical details. To date their procedure has been concerned almost exclusively with enamel2 and hence a very sensitive method was needed for calipering loss by the test specimens. While the procedure has been used to a limited extent on dentin, it is not adequate for the purpose as will be hereinafter explained. PROCEDURES (a) Crosswise brushing machines. Whole teeth, toothbrushes and crosswise brushing on the labial or lingual surfaces were used as in the previous method (1), but new machines have been subsequently designed with 4 times as much capacity, and a shadowgraphic procedure has been substituted for the tedious microscopic method of measuring the depth of abrasion.