Syringin from stem bark of Fraxinus rhynchophylla protects Aβ(25–35)-induced toxicity in neuronal cells

Abstract

The medicinal herb Jinpi, derived from the dried stem barks of Fraxinus rhynchophylla belonging to Oleaceae is widely used as a variety of Korean folk remedies for anti-inflammatory, febricide, antidiarrhea, and antileukorrhea diseases. In the course of screening antidementia agents from natural products, F. rhynchophylla showed significant inhibitory activity toward Abeta(25-35)-induced neuronal cell death. An active principle was isolated and identified as syringin. When the neuroblastoma cells were exposed to 50 microM Abeta(25-35), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction rate (survival rate) decreased to 60.21 +/- 2.16% over control while syringin treated ones recovered cell viability up to 79.12 +/- 1.39% at 20 microM. In addition, 20 microM syringin almost completely removed Abeta(25-35)-induced reactive oxygen species. The neuroprotective effect of syringin seemed to be originated from the reduction of apoptosis since decrease in caspase-3 activity and expression, reduction in cleaved PARP, and DNA fragmentation were observed. These results suggest that F. rhynchophylla and syringin are expected to be useful for preventing Abeta(25-35)-induced neuronal cell damage.

Hepatoprotective effects of syringin on fulminant hepatic failure induced by D‐galactosamine and lipopolysaccharide in mice

“…Meanwhile, a reduction in apoptotic cells and suppression of caspase‐3 activity were observed in syringin‐pretreated groups, suggesting syringin might relieve liver injury through inhibiting hepatocellular apoptosis. This result is consistent with a previous report, in which syringin inhibited apoptosis and caspase‐3 activity induced by Aβ25‐35 in neuronal cells (Yang et al ., ).…”

Chemical Constituents From the Seeds of Annona Reticulata L. In Vietnam

“…The signal at δ C 56.4 (3,5-OCH 3 ) indicated two O-methyl carbons. On the basis of the 1 H and 13 C NMR data, compound 3 was assumed as eleutheroside B (syringin) and belongs to the phenylpropanoid glucosides [7]. 13 C-NMR, HMBC spectra between 3 and 4 displayed that the CH 2 OH (C-9) group was oxidized to aldehyde (δ H 9.66 (1H, d, J = 7.5 Hz) and δ C 196.0) and these were also supported of determining the molecular formula.…”

Hepatoprotective standardized EtOH–water extract from the seeds of Fraxinus rhynchophylla Hance

“…The extracts and the identified compounds from F. rhynchophylla Hance had been reported anti-oxidative activity 30, 31, 32. MDA is an aldehyde, which is a product of polyunsaturated fatty acid peroxidation, and is a highly toxic molecule considered as more than just a marker of lipid peroxidation.…”

“…Many natural products such as secoiridoid glucosides, coumarins, lignans, sesquilignans, and coumarinolignans have been identified from this plant 18, 19, 20, 21, 22, 23. Recently, several pharmacological activities of phytochemical constituents isolated from the barks and leaves of F. rhynchophylla have been carried out, including anti-diabetes effects,24, 25, 26, 27, 28 anti- Toxoplasma gondii effects, 29 including antioxidant enzymes, 30 inhibiting amyloid-β-induced neuronal cell damage, 31 and inhibiting nitric oxide synthesis activities 32 . Hydrangeside B, along with other secoiridoid glucosides showed hepatoprotective activities against dl -galactosamine-induced toxicity in human hepatocyte HL-7702 (HL-7702) cells 33 .…”