System Integration, Modelling, and Simulation for Automation of Multiple Microfluidic Stream Based Bio-manipulation

Abstract

This paper proposes system integration, modeling, and simulation for automation of multiple microfluidic stream based bio-manipulation (MMSM) system. It uses micro hydrodynamics and lab on chip (LOC) technology. Our method can implement the functions of micro manipulation and micro assembly of bio-objects, such as biological cells in an open space without contact. Compared to other conventional bio-micro-manipulation and assembly methods, this system manipulates micro objects by automatically controlling multiple microfluidic streams onto them from various directions. The advantages of this method are that it performs open space, multi-function, multi-scale, multi-degree-offreedom, and non-invasive automatic 3D manipulation. These microfluidic streams are generated simultaneously from multiple orifices. By regulating the parameters of the microfluidic stream, such as the position and number of operating orifices and the flow rate, the direction and velocity of the object can be controlled. The capable of this system was demonstrated with micro objects.

A Method of Three-Dimensional Micro-Rotational Flow Generation for Biological Applications

“…For example, in our previous work, we reported a three-dimensional microfluidic manipulation system consisting of nine high-specification pumps, a multiple-layer structure microfluidic chip with nine orifices, a control system with a D/A board, a computer and a self-made complex controller [ 21 ]. The system functioned by using a self-made control algorithm [ 22 ]. However, for practical use in the biological field, a more convenient system requiring no extra chip design, multiple components or complex control system to realize the purposes of capturing, moving and rotating cells is desired.…”