System of Color Wheels for Streptomycete Taxonomy

Tresner, H. D.; Backus, E. J.

doi:10.1128/am.11.4.335-338.1963

Cited by 76 publications

(16 citation statements)

References 1 publication

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“…Isolates were morphologically characterized by determining the production of diffuse pigments and the color of aerial and substrate mycelium on TSA medium and then classified into a color class [ 22 ]. Selected isolates were tested for enzyme activities (proteolytic, lipolytic, amylolytic and cellulolytic) using TSA medium supplemented with milk powder (1% w / v ), glycerol tributyrate (1% v / v ), Starch azure (0.65% w / v ) or carboxymethyl cellulose (2.6% w / v ) [ 23 , 24 , 25 ].…”

Section: Methodsmentioning

confidence: 99%

Biodiversity of Actinomycetes from Heavy Metal Contaminated Technosols

2021

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Technosols are artificial soils generated by diverse human activities and frequently contain toxic substances resulting from industrial processes. Due to lack of nutrients and extreme physico-chemical properties, they represent environments with limited bacterial colonization. Bacterial populations of technosols are dominated usually by Actinobacteria, including streptomycetes, known as a tremendous source of biotechnologically important molecules. In this study, the biodiversity of streptomycete-like isolates from several technosols, mainly mine soils and wastes (landfills and sludge) in Slovakia, was investigated. The combination of basic morphological and biochemical characterisations, including heavy metal resistance determination, and molecular approaches based on 16S rRNA gene analysis were used for the identification of the bacterial strains. From nine isolates of Actinobacteria collected from different habitats, one was found to represent a new species within the Crossiella genus. Eight other isolates were assigned to the genus Streptomyces, of which at least one could represent a new bacterial species. Some isolates showed high resistance to Pb, Zn, Cu or Ni. The most tolerated metal was Pb. The results obtained in this study indicate that technosols are a prospective source of new actinomycete species resistant to heavy metals what underlines their bioremediation potential.

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Section: Methodsmentioning

confidence: 99%

Biodiversity of Actinomycetes from Heavy Metal Contaminated Technosols

2021

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“…The spore surface was examined using Em10 Carl-Zeiss transmission electron microscope [22]. The cultural characteristics of the strains were tested on the basis of the methods used in the International Streptomyces Project (ISP), using the media recommended by Shirling and Gottlieb [21].…”

Section: Morphological Physiological and Biochemical Characterizationmentioning

confidence: 99%

Study of exopolysaccharide produced by Streptomyces rochie strain OF1 and its effect as ameliorative on osteoarthritis in rats via inhibiting TNF-α/COX2 pathway

Mohamed

Awady

Abdelhamid

et al. 2023

Journal of Genetic Engineering and Biotechnology

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Background Carbohydrates are known as the main natural products of life activities. Results Streptomyces rochie strain OF1 isolated from a mangrove tree produced exopolysaccharide S5 (EPSS5) (14.2 gl−1) containing uronic acid 21.98% sulfate content of 11.65 mg/ml, and a viscosity of 1.35 mm2/s. while total hexose amine content was 24.72%. The high performance liquid chromatography (HPLC) analysis of mono sugars revealed that EPS was composed of manouronic acid, glucuronic acid, xylose, and fructose at a molar ratio of 1.0:0.5:1.0:2.0, respectively. It showed that the whole antioxidant activity was 92.06%. It showed antibacterial activity against Staphylococcus aureus, and E. coli, MRSA and Klebsiella pneumoniae. But, EPSS5 displayed low antifungal activity against Candida albicans. While no antifungal activity has been detected against Aspergillus niger. EPSS5 has antibiofilm action that is noticeable toward S. aureus with an inhibition ratio of biofilm up to 50%. Effect of EPS on serum levels of TNF-α and COX2 by 2 fold and 1.9 fold of EPS reduced serum levels of Tumor necrosis factor-α (TNF-α) by 38%, 12%, 49%, and Cyclooxygenase-2 (COX2) by 61%, 34%, and 62%, respectively. By affected of EPSS5 on arthritis in rats stimulated by carrageenan. Conclusions Administration of EPS ameliorated carrageen-induced elevation in inflammatory mediators; TNF-α/COX and suppressed the expressions of metalloproteinase 9 (MMP9) by 68%, 86%, and 75% correspondingly in comparison to the group of carrageenans. Then again, therapy involving a high dose only reduced MMP9 level by 57%, compared to free drug suggesting that EPSS5 is a good inhibitor of the MMP9, as it brought MMP9 back to normal levels via the signaling pathway.

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“…Isolates were identified according to the ISP method (Shirling and Gottlieb, 1966) mycelium and spores was recorded and isolates were classified according to the Tresner -Backus colour wheel (Tresner-Backus, 1963).…”

Section: Morphological and Physiological Testsmentioning

confidence: 99%

“…Yeast malt extract (ISP-2) agar plates were inoculated in triplicate with each Streptomyces isolate for each temperature. The isolates were incubated at different temperatures (15,20,25,30, 35 and 40°C) for 14 days. Minimum and maximum growth responses of each isolate were recorded.…”

Section: Growth Response At Different Temperaturesmentioning

confidence: 99%

Isolation of pathogenic Streptomyces spp. and determination of variability among the strains collected from potato

Saha¹,

Naher²,

Haque³

et al. 2022

J biosci agric res

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A study was undertaken during 2008-2009, 2009-2010 and 2010-11 cropping seasons to isolate the variability(s) of Streptomyces spp. from lesion of common scab affected potato tubers in the Northern parts of Bangladesh (Rangpur division). There were twelve different pathogenic species isolated, which were different for colony colour, spore colour, spore ornamentation, spore chain morphology, other physiological characteristics (temperature, pH, melanin production, colour secret in oatmeal broth medium, carbon utilization and growth response in NaCl solution) and pathogenic reaction. Overall all collected isolates grow well within temperature range of 25°C to 30°C. In the present investigation, it was observed that isolates S2 and S11 could grow at pH 4.50. It was found that the growth of isolates increased as the pH value of medium increased. Considering all the characteristics of different isolates, isolate S5 agreed with the description of S. scabies having smooth, grey spores borne in spiral chains, producing melanin and utilizing eight recommended ISP diagnostic sugars with raffinose as a sole source of carbon. Rest eleven isolates could not identify.

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System of Color Wheels for Streptomycete Taxonomy

Cited by 76 publications

References 1 publication

Biodiversity of Actinomycetes from Heavy Metal Contaminated Technosols

Biodiversity of Actinomycetes from Heavy Metal Contaminated Technosols

Study of exopolysaccharide produced by Streptomyces rochie strain OF1 and its effect as ameliorative on osteoarthritis in rats via inhibiting TNF-α/COX2 pathway

Isolation of pathogenic Streptomyces spp. and determination of variability among the strains collected from potato

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