Systematic Analysis and Identification of Drought-Responsive Genes of the CAMTA Gene Family in Wheat (Triticum aestivum L.)

Abstract

The calmodulin-binding transcription activator (CAMTA) is a Ca2+/CaM-mediated transcription factor (TF) that modulates plant stress responses and development. Although the investigations of CAMTAs in various organisms revealed a broad range of functions from sensory mechanisms to physiological activities in crops, little is known about the CAMTA family in wheat (Triticum aestivum L.). Here, we systematically analyzed phylogeny, gene expansion, conserved motifs, gene structure, cis-elements, chromosomal localization, and expression patterns of CAMTA genes in wheat. We described and confirmed, via molecular evolution and functional verification analyses, two new members of the family, TaCAMTA5-B.1 and TaCAMTA5-B.2. In addition, we determined that the expression of most TaCAMTA genes responded to several abiotic stresses (drought, salt, heat, and cold) and ABA during the seedling stage, but it was mainly induced by drought stress. Our study provides considerable information about the changes in gene expression in wheat under stress, notably that drought stress-related gene expression in TaCAMTA1b-B.1 transgenic lines was significantly upregulated under drought stress. In addition to providing a comprehensive view of CAMTA genes in wheat, our results indicate that TaCAMTA1b-B.1 has a potential role in the drought stress response induced by a water deficit at the seedling stage.

Comprehensive identification and expression analysis of CAMTA gene family in Phyllostachys edulis under abiotic stress

“…Phylogenetic analysis of amino acid sequences of P. edulis CAMTA , O. sativa CAMTA , Arabidopsis CAMTA , Z. mays CAMTA and B. distachyon CAMTA was performed, and we classified the amino acid sequences of PeCAMTAs into five subbranches (I~V) ( Fig. 1 ) according to Wang et al (2022) classification method, among which the protein sequences of Arabidopsis CAMTA genes were classified into one subclade, and the amino acid sequences of O. sativa , Z. mays and P. edulis CAMTA genes were grouped into one subclade. It is more closely related to O. sativa and Z. mays , and more distantly related to Arabidopsis .…”

“…Recent studies on wheat confirmed that the expression of TaCAMTA1a-B and TaCAMTA1b-B. A total of 1 was down- and up-regulated, respectively, in response to drought stress to maintain normal physiological functions associated with the plant, and wheat CAMTA gene family members also contain a large number of MYB elements ( Wang et al, 2022 ). The previous study also gave us ideas for subsequent stress experiments, and it became important to verify the expression of PeCAMTAs in response to drought stress.…”

“…Interestingly, TaCAMTA was found to regulate mainly the drought stress response during the seedling stage of wheat, and TaCAMTA1b-B. 1 plays an essential role in the response to drought stress caused by water deficit in the nursery stage ( Wang et al, 2022 ).…”

A NAC Transcription Factor from ‘Sea Rice 86′ Enhances Salt Tolerance by Promoting Hydrogen Sulfide Production in Rice Seedlings

“…High salinity induces changes in the expression of many stress-related genes [ 8 ]. Among these, various families of transcription factors (TFs) (e.g., NAC, AP2/ERF, MYB, WRKY, bZIP, bHLH and CAMTA) have been identified and characterized [ 9 , 10 , 11 , 12 , 13 , 14 , 15 ]. The NAC TFs comprise one of the largest families of plant-specific TFs with 117 and 151 predicted members in Arabidopsis and rice [ 16 ], respectively.…”

Genome-wide analysis of transmembrane 9 superfamily genes in wheat (Triticum aestivum) and their expression in the roots under nitrogen limitation and Bacillus amyloliquefaciens PDR1 treatment conditions

“…No duplication of TraesTM9SF genes in the wheat genome was recorded, and 177 TFs were 1 Introduction Wheat (T. aestivum) is an important food crop consumed worldwide (Ismagul et al, 2014). Diverse factors, including drought, salinity, nutrient limitation, pest attack, and extreme temperatures, can affect the growth, development, quality, and yield of wheat and other crops (Zhang et al, 2021;Ahlawat et al, 2022;Dissanayake et al, 2022;Fang et al, 2022a;Venzhik et al, 2022;Wang et al, 2022;Yue et al, 2022;Yu et al, 2022). It is essential to examine the mechanisms involved in wheat growth under such conditions to improve productivity; it is also vital to boost wheat growth under these conditions.…”