Systematic analysis of novel psychoactive substances. II. Development of a screening/confirmatory LC-QqQ-MS/MS method for 800+ compounds and metabolites in urine

Kimble, Ashley N.; DeCaprio, Anthony P.

doi:10.1016/j.forc.2019.100189

Cited by 17 publications

(12 citation statements)

References 21 publications

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“…The major challenge in the analysis of NPS resides in the diversity of structures and physicochemical properties among different NPS classes and within a single NPS class 11,16 . Several methods are already available in the literature to detect and/or quantify NPS in the main biological matrices 20–26 and particularly in whole blood, 27–31 though methods including a high number of compounds pertaining to different classes are still scarce 22 .…”

Section: Discussionmentioning

confidence: 99%

“…Due to constantly evolving novel compounds, laboratories are forced to choose between applying high sensitivity and specificity target methods for a small set of molecules, which is time and material‐consuming, or to perform broad range screening methods including many substances, which have to be confirmed by for further (quantitative) target analyses. Screening methodology is complicated by the fact that chemical diversity usually requires adapted sample preparation, mobile phases, and chromatographic and MS/MS conditions in order to achieve good identification power and usually only allows a qualitative or semi‐quantitative approach when covering a high number of compounds 9,11,16 …”

Section: Introductionmentioning

confidence: 99%

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Development and validation of a rapid LC‐MS/MS method for the detection of 182 novel psychoactive substances in whole blood

Giorgetti

Barone

Pelletti

et al. 2021

Drug Testing and Analysis

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Introduction: The analysis of novel psychoactive substances (NPS) represents a challenge in forensic toxicology, due to the high number of compounds characterized by different structures and physicochemical properties both among different subclasses and within a single subclass of NPS. The aim of the present work is the development and validation of a targeted liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the detection of NPS in whole blood. Materials and methods:A protein-precipitation based LC-MS/MS method for the detection of more than 180 NPS was developed and validated by assessing the following parameters: selectivity, linearity, accuracy, precision, limit of detection (LOD) and of quantification (LOQ) recovery, and matrix effect. Then, the method was applied to real forensic samples. Results:The method allowed the identification of 132 synthetic cannabinoids, 22 synthetic opioids, and 28 substances among synthetic cathinones, stimulants, and other drugs. Validation was successfully achieved for most of the compounds. Linearity was in the range of 0.25-10 ng/ml for synthetic cannabinoids and 0.25-25 ng/ml for other drugs. Accuracy and precision were acceptable according to international guidelines. Three cases tested positive for fentanyl and ketamine, in the setting of emergency room administration. Conclusions:The present methodology represents a fast, not expensive, wide-panel method for the analysis of more than 180 NPS by LC-MS/MS, which can be profitably applied both in a clinical context and in postmortem toxicology.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development and validation of a rapid LC‐MS/MS method for the detection of 182 novel psychoactive substances in whole blood

Giorgetti

Barone

Pelletti

et al. 2021

Drug Testing and Analysis

View full text Add to dashboard Cite

show abstract

“…These techniques can allow for sampling across a range of biological samples, including blood, urine, hair, 223 saliva, 224 urban wastewater and dried blood samples. 225 , 226 Samples for analysis require laboratory pre-preparation before being used for these techniques, though so-called ‘dilute and shoot’ techniques are being validated to allow for more rapid preparation of biological samples for liquid chromatography mass spectrometry. 227 , 228 Liquid chromatography with quadrupole time of flight mass spectrometry (LC-QTOF MS) has demonstrated some superiority to gas chromatography mass spectroscopy (GC MS) in detecting most forms of NPS within serum samples.…”

Section: Laboratory Testingmentioning

confidence: 99%

New psychoactive substances: a review and updates

Shafi

Berry

Sumnall

et al. 2020

Therapeutic Advances in Psychopharmacology

193

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New psychoactive substances (NPS) are a heterogeneous group of substances. They are associated with a number of health and social harms on an individual and societal level. NPS toxicity and dependence syndromes are recognised in primary care, emergency departments, psychiatric inpatient and community care settings. One pragmatic classification system is to divide NPS into one of four groups: synthetic stimulants, synthetic cannabinoids, synthetic hallucinogens and synthetic depressants (which include synthetic opioids and benzodiazepines). We review these four classes of NPS, including their chemical structures, mechanism of action, modes of use, intended intoxicant effects, and their associated physical and mental health harms. The current challenges faced by laboratory testing for NPS are also explored, in the context of the diverse range of NPS currently available, rate of production and emergence of new substances, the different formulations, and methods of acquisition and distribution.

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“…In addition, the phenomenon of the new psychoactive substances are also motifs of concern in the workplace that should not be disregarded [51]. Indeed, the consumption of new psychoactive substances poses a significant risk to public health and a challenge to national and international drug policies for occupational medicine, and only very recently have laboratories begun to offer screening and confirmation analysis for new psychoactive substances at lower costs [105].…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Abuse of Licit and Illicit Psychoactive Substances in the Workplace: Medical, Toxicological, and Forensic Aspects

Dinis-Oliveira

Magalhães

2020

JCM

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About one-third of adult life is spent in the workplace. The use of psychoactive substances is a major preventable cause of morbidity and mortality. The consumption of psychoactive substances during or outside working hours greatly increases the frequency and severity of labor accidents, as well as the workers’ poor general state of health and productivity, implying higher costs for enterprises. It is the responsibility of organizations to ensure the safety and health of their workers. These cannot be limited to traditional routine clinical exams, as other aspects also have an impact on health. Thus, prevention and intervention in the consumption of psychoactive substances (e.g., ethanol, opioids, central nervous system stimulants or depressants, hallucinogens, Cannabis derivatives, dissociative substances, and inhalants) in labor activity should be considered as an investment of organizations and not as a cost, in view of the professional, personal, and family advantages for workers and employers, with a potential impact on productivity, security, health, and quality of life at work. Despite the extensive literature on the subject, each article generally focuses on one or another aspect of a very specific nature, not tackling the problem in a holistic way by confronting clinical, safety, and legal issues. This article presents a reflection on the legal, laboratorial, clinical, ethical, forensic, and safety concerns related to the consumption of psychoactive substances in the workplace, and can be a cross-cutting contribution to occupational medicine, forensic medicine, and insurance medicine, as well as for entrepreneurs, lawyers, judges, workers, and technicians from the public and private sectors that develop projects in this area. This discussion is based on general principles established internationally and highlights the role of the occupational healthcare system and other decision-making actors in the prevention and supervision of workplace psychoactive consumption.

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Systematic analysis of novel psychoactive substances. II. Development of a screening/confirmatory LC-QqQ-MS/MS method for 800+ compounds and metabolites in urine

Cited by 17 publications

References 21 publications

Development and validation of a rapid LC‐MS/MS method for the detection of 182 novel psychoactive substances in whole blood

Development and validation of a rapid LC‐MS/MS method for the detection of 182 novel psychoactive substances in whole blood

New psychoactive substances: a review and updates

Abuse of Licit and Illicit Psychoactive Substances in the Workplace: Medical, Toxicological, and Forensic Aspects

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