Systematic analysis of the non-specific lipid transfer protein gene family in Nicotiana tabacum reveal its potential roles in stress responses

Yang, Yongxia; Wang, Peng; Cao, Peijian; Xie-feng, YE; Li, Qingchang

doi:10.1016/j.plaphy.2022.01.002

Cited by 22 publications

(15 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In particular, ShnsLTPIV.3 expression was dramatically upregulated across all time points in both sugarcane cultivars with exogenous SA treatment. Conversely, some ShnsLTPs (e.g., ShnsLTPI.10/Gb.1) might play negative regulators in sugarcane trigged by SA stress, which is consistent with previous findings that NtLTPI.1 (homolog of ShnsLTPI.19) (Jin et al, 2015) and NtLTPI.34/NtLTPVIII.1 (Yang et al, 2022) in tobacco are strongly induced by SA. In contrast, ScNsLTP (homolog of ShnsLtpII.1) in sugarcane was inhibited by exogenous SA and downregulated across all time points, as part of the antagonistic regulation involving the MeJA signaling molecule (Chen et al, 2021).…”

Section: Discussionsupporting

confidence: 92%

“…These genes were systematically identified at a genomewide level. Highly variable numbers of nsLTPs were also present between plant genus members such as N. sylvestris (50) vs. N. tabacum (100) (Yang et al, 2022) Frontiers in Plant Science 09 frontiersin.org L. Moench) (Wang et al, 2012) as bait to search for nsLTP members in S. spontaneum and identified 21 gene members of nsLTP Type I. High variability of nsLTP family members among different plant species might be due to different polyploidization or the duplication of entire genomes during plant evolution (Van de Peer et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…The nsLTPs are encoded by a complex of multigene families that have varying numbers of genes and carry out key biological functions in a wide of range of plants, such as Arabidopsis , Oryza sativa , and Triticum aestivum ( Boutrot et al, 2008 ), S. bicolor and Zea mays ( Wei and Zhong, 2014 ), G. hirsutum ( Li et al, 2016 ), Sesamum indicum ( Song et al, 2021 ), Nicotiana sylvestris and Nicotiana tomentosiformis ( Yang et al, 2022 ), Nicotiana tabacum ( Yang et al, 2022 ), and B. napus ( Liang et al, 2022 ; Xue et al, 2022 ). The number of nsLTP family genes in plants varied from 13 VvnsLTPs in Vitis vinifera ( Kobayashi et al, 2011 ) to 461 TansLTPs in T. aestivum ( Kouidri et al, 2018 ; Fang et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…These genes were systematically identified at a genome-wide level. Highly variable numbers of nsLTPs were also present between plant genus members such as N. sylvestris (50) vs. N. tabacum (100) ( Yang et al, 2022 ) and Brassica rapa (127) vs. B. napus (283). In this study, we identified 71 nsLTP genes with 66 alleles in S. spontaneum AP85-441; Type I had largest number of nsLTP genes (22).…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Systematic and functional analysis of non-specific lipid transfer protein family genes in sugarcane under Xanthomonas albilineans infection and salicylic acid treatment

Zhao²,

Shi³

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

Plant non-specific lipid transfer proteins (nsLTPs) are small basic proteins that play a significant regulatory role in a wide range of physiological processes. To date, no genome-wide survey and expression analysis of this gene family in sugarcane has been performed. In this study we identified the nsLTP gene family in Saccharum spontaneum and carried out expression profiling of nsLTPs in two sugarcane cultivars (Saccharum spp.) that have different resistance to leaf scald caused by Xanthomonas albilineans (Xa) infection. The effect of stress related to exogenous salicylic acid (SA) treatment was also examined. At a genome-wide level, S. spontaneum AP85-441 had 71 SsnsLTP genes including 66 alleles. Tandem (9 gene pairs) and segmental (36 gene pairs) duplication events contributed to SsnsLTP gene family expansion. Five SsnsLTP proteins were predicted to interact with five other proteins. Expression of ShnsLTPI.8/10/Gb.1 genes was significantly upregulated in LCP85-384 (resistant cultivar), but downregulated in ROC20 (susceptible cultivar), suggesting that these genes play a positive regulatory role in response of sugarcane to Xa infection. Conversely, ShnsLTPGa.4/Ge.3 appears to act as a negative regulator in response Xa infection. The majority (16/17) of tested genes were positively induced in LCP85-384 72 h after SA treatment. In both cultivars, but particularly in LCP85-384, ShnsLTPIV.3/VIII.1 genes were upregulated at all time-points, suggesting that the two genes might act as positive regulators under SA stress. Meanwhile, both cultivars showed downregulated ShnsLTPGb.1 gene expression, indicating its potential negative role in SA treatment responses. Notably, the ShnsLTPGb.1 gene had contrasting effects, with positive regulation of gene expression in response to Xa infection and negative regulation induced by SA stress. Together, our results provide valuable information for elucidating the function of ShnsLTP family members under two stressors and identified novel gene sources for development of sugarcane that are tolerant of environmental stimuli.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Systematic and functional analysis of non-specific lipid transfer protein family genes in sugarcane under Xanthomonas albilineans infection and salicylic acid treatment

Zhao²,

Shi³

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…An increasing number of studies have shown that plant nsLTP genes play an important role in the response to biotic and abiotic stresses and in plant growth and development [ 11 , 18 , 21 , 23 , 25 , 28 , 29 , 31 ]. To date, with the availability and feasibility of plant reference genomes, the nsLTP gene family has been identified at the genome-wide level in various plant species, including Arabidopsis, Gossypium , sesame, cotton, wheat, potato, tomato, tobacco, barley, maize, cabbage and Chinese cabbage [ 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 58 , 59 , 60 , 61 ]. In this study, 283, 127, 151 and 106 nsLTP genes were identified in B. napus , B. rapa , B. oleracea and A. thaliana genomes, respectively.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Expression Analysis of nsLTP Gene Family in Rapeseed (Brassica napus) Reveals Their Critical Roles in Biotic and Abiotic Stress Responses

Xue

Zhang

Shan

et al. 2022

IJMS

View full text Add to dashboard Cite

Non-specific lipid transfer proteins (nsLTPs) are small cysteine-rich basic proteins which play essential roles in plant growth, development and abiotic/biotic stress response. However, there is limited information about the nsLTP gene (BnLTP) family in rapeseed (Brassica napus). In this study, 283 BnLTP genes were identified in rapeseed, which were distributed randomly in 19 chromosomes of rapeseed. Phylogenetic analysis showed that BnLTP proteins were divided into seven groups. Exon/intron structure and MEME motifs both remained highly conserved in each BnLTP group. Segmental duplication and hybridization of rapeseed’s two sub-genomes mainly contributed to the expansion of the BnLTP gene family. Various potential cis-elements that respond to plant growth, development, biotic/abiotic stresses, and phytohormone signals existed in BnLTP gene promoters. Transcriptome analysis showed that BnLTP genes were expressed in various tissues/organs with different levels and were also involved in the response to heat, drought, NaCl, cold, IAA and ABA stresses, as well as the treatment of fungal pathogens (Sclerotinia sclerotiorum and Leptosphaeria maculans). The qRT-PCR assay validated the results of RNA-seq expression analysis of two top Sclerotinia-responsive BnLTP genes, BnLTP129 and BnLTP161. Moreover, batches of BnLTPs might be regulated by BnTT1 and BnbZIP67 to play roles in the development, metabolism or adaptability of the seed coat and embryo in rapeseed. This work provides an important basis for further functional study of the BnLTP genes in rapeseed quality improvement and stress resistance.

show abstract

Identification of potato Lipid transfer protein gene family and expression verification of drought genes StLTP1 and StLTP7

Wang

Song

Lin³

et al. 2023

Plant Direct

View full text Add to dashboard Cite

Lipid transfer proteins (LTPs) are widely distributed in plants and play an important role in the response to stress. Potato (Solanum tuberosum L.) is sensitive to a lack of water, and drought stress is one of the limiting factors for its yield. Therefore, mining candidate functional genes for drought stress and creating new types of potato germplasm for drought resistance is an effective way to solve this problem. There are few reports on the LTP family in potato. In this study, 39 members of the potato LTP family were identified. They were located on seven chromosomes, and the amino acid sequences encoded ranged from 101 to 345 aa. All 39 family members contained introns and had exons that ranged from one to four. Conserved motif analysis of potato LTP transcription factors showed that 34 transcription factors contained Motif 2 and Motif 4, suggesting that they were conserved motifs of potato LTP.Compared with the LTP genes of homologous crops, the potato and tomato (Solanum lycopersicum L.) LTPs were the mostly closely related. The StLTP1 and StLTP7 genes were screened by quantitative reverse transcription PCR combined with potato transcriptome data to study their expression in tissues and the characteristics of their responses to drought stress. The results showed that StLTP1 and StLTP7 were upregulated in the roots, stems, and leaves after PEG 6000 stress. Taken together, our study provides comprehensive information on the potato LTP family that will help to develop a framework for further functional studies.expression analysis, genome-wide identification, LTP, potato) is one of the most important food crops and economic crops in China, and the amounts of its area and yield rank first in the world. Inner Mongolia is one of the primary bases for the production of potato seed and commercial potatoes in China. The annual rainfall is approximately 200-350 mm, and the region is arid and semiarid (du et al., 2013;Zhang, Li, et al., 2022). Drought stress is Dan Wang and Jian Song contributed equally to this work.

show abstract

Systematic analysis of the non-specific lipid transfer protein gene family in Nicotiana tabacum reveal its potential roles in stress responses

Cited by 22 publications

References 32 publications

Systematic and functional analysis of non-specific lipid transfer protein family genes in sugarcane under Xanthomonas albilineans infection and salicylic acid treatment

Systematic and functional analysis of non-specific lipid transfer protein family genes in sugarcane under Xanthomonas albilineans infection and salicylic acid treatment

Genome-Wide Identification and Expression Analysis of nsLTP Gene Family in Rapeseed (Brassica napus) Reveals Their Critical Roles in Biotic and Abiotic Stress Responses

Identification of potato Lipid transfer protein gene family and expression verification of drought genes StLTP1 and StLTP7

Contact Info

Product

Resources

About